LOCUS       sequence-231079-        2158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for blank part (random 50bp 
            sequence) - Please see Supplemental Documents for annotated Genbank 
            file.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2158)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C40m
FEATURES             Location/Qualifiers
     source          1..2158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    66..86
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(92..109)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(263..851)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(343..362)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1022..1813,1814..1882))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1645..1664
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1883..1987)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2055..2073
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2077..2120)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2123..2144
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatgggccca tatctggaaa tagacgccaa gttcaatccg tattccgacg tacgaggtag
       61 agacctacta gtagcggccg ctgcaggctt cctcgctcac tgactcgctg cgctcggtcg
      121 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      181 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      241 aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa
      301 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      361 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      421 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      481 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      541 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      601 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
      661 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
      721 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
      781 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
      841 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
      901 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
      961 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     1021 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     1081 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     1141 ccagtgctgc aatgataccg cgcgacccac gctcaccggc tccagattta tcagcaataa
     1201 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     1261 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     1321 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     1381 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     1441 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     1501 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     1561 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     1621 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     1681 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     1741 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     1801 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     1861 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     1921 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     1981 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     2041 cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag
     2101 ctttcgctaa ggatgatttc tggaattcgc ggccgcttct agagactagt gggtctca
//
