LOCUS       sequence-231083-        2840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for RpaR* (improved RpaR 
            quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). 
            Please see Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2840)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C61m
FEATURES             Location/Qualifiers
     source          1..2840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    748..768
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(774..791)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(945..1533)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1025..1044)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1704..2495,2496..2564))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2327..2346
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2565..2669)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2737..2755
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2759..2802)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2805..2826
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatgatgatc gtcggcgaag atcagctttg gggacggcgt gcgctggaat tcgtcgattc
       61 cgtcgaacgg ctcgaggcgc cggcgctgat cagccggttc gaatcgctga tcgcgagctg
      121 cggatttacc gcctacatca tggccggcct gccgtcgcgc aatgccggac taccggagct
      181 gacgctggcc aatggctggc cgcgagactg gttcgatctg tatgtcagcg aaaacttcag
      241 cgcggtcgat ccggtgccgc gccacggcgc taccacggtt catcctttcg tatggtccga
      301 tgcaccctac gaccgcgacc gtgatccggc cgcccaccgg gtcatgaccc gggcggcgga
      361 attcggactg gtcgagggtt actgcattcc gctgcactac gacgacggta gcgccgcgat
      421 cagcatggcc ggcaaggatc cggacctcag cccggccgcg cgcggcgcga tgcagctggt
      481 cagcatctac gcgcatagtc gcctgcgcgc actcagccgg ccaaagccga tccggcgcaa
      541 ccggctcacg ccgcgcgagt gcgagatcct gcaatgggca gcgcagggca agaccgcctg
      601 ggaaatctcg gtaatcctct gcatcaccga acgcacggtg aaattccatc tgatcgaagc
      661 cgcccgcaag ctcgacgccg ccaaccgcac cgcggcggtt gccaaggcat tgacgctcgg
      721 attgatccgt ttgtgaaggt agagacctac tagtagcggc cgctgcaggc ttcctcgctc
      781 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
      841 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
      901 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca caggctccgc
      961 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1021 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1081 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1141 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1201 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1261 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1321 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1381 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1441 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1501 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1561 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     1621 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     1681 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     1741 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     1801 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgcgaccc acgctcaccg
     1861 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     1921 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     1981 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     2041 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     2101 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     2161 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     2221 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     2281 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     2341 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     2401 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     2461 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     2521 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     2581 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     2641 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     2701 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat
     2761 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc gcggccgctt
     2821 ctagagacta gtgggtctca
//
