LOCUS       sequence-231084-        2813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for TraR(W) (improved TraR 
            quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). 
            Please see Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2813)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C62m
FEATURES             Location/Qualifiers
     source          1..2813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    721..741
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(747..764)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(918..1506)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(998..1017)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1677..2468,2469..2537))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2300..2319
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2538..2642)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2710..2728
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2732..2775)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2778..2799
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatgatgcag cactggctgg ataagctgac tgatctggcg gcaattgagg gtgatgaatg
       61 cattctgaaa actggtctgg cggatattgc ggaccacttc ggttttaccg gttacgcgta
      121 cctgcacatc cagcaccgtc atattaccgc tgtgaccaac taccaccgtc aatggcaatc
      181 cacctacttc gataaaaaat tcgaggcgct ggacccggtg gttaagcgtg cgcgttctcg
      241 caaacacatc ttcacttgga gcggtgaaca cgaacgtcca actctgagca aagacgaacg
      301 cgcgttctac gaccacgcgt ccgactttgg tatccgttct ggcatcacca ttccgattaa
      361 aacggccaac ggtttcatgt ctatgttcac catggcaagc gataaaccgg ttatcgacct
      421 ggaccgtgaa atcgacgccg tagctgcggc ggcgaccatc ggtcaaattc acgcgcgtat
      481 ctcctttctg cgcacgaccc cgacggcgga ggacgctgca tggctggatc ctaaggaagc
      541 tacttacctg cgttggatcg ccgtaggcaa aaccatgtgg gaaatcgcag atatcgaagg
      601 cgttaaatac aattccgtcc gcgtaaaact gcgcgaagct atgaaacgct tcgacgtacg
      661 ttctaaagcc cacctgacgg ccctggctat ccgtcgcaag ctgatctgaa ggtagagacc
      721 tactagtagc ggccgctgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      781 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      841 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      901 gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga
      961 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1021 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1081 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1141 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1201 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1261 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1321 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1381 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1441 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1501 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1561 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     1621 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     1681 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     1741 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     1801 gctgcaatga taccgcgcga cccacgctca ccggctccag atttatcagc aataaaccag
     1861 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     1921 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     1981 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     2041 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     2101 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     2161 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     2221 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     2281 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     2341 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     2401 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     2461 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     2521 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     2581 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     2641 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     2701 acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc
     2761 gctaaggatg atttctggaa ttcgcggccg cttctagaga ctagtgggtc tca
//
