LOCUS       sequence-231088-        4379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for Bxb1-mScarleti-LAA (Bxb1 
            integrase tagged with an mScarlet-I reporter and an LAA degradation 
            tag). Please see Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4379)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C67m
FEATURES             Location/Qualifiers
     source          1..4379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2..1504
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="MVRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDL
                     DVSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKL
                     VVSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWG
                     YLPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQ
                     GREPQGREWSATALKRSMISGAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRA
                     ELVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGT
                     VAMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRA
                     GSPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRS
                     MNVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     misc_feature    2287..2307
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(2313..2330)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2484..3072)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2564..2583)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3243..4034,4035..4103))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3866..3885
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4104..4208)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4276..4294
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(4298..4341)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    4344..4365
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatggtgaga gccctggtag tcatccgcct gtcccgcgtc accgatgcta cgacttcacc
       61 ggagcgtcag ctggagtctt gccagcagct ctgcgcccag cgcggctggg acgtcgtcgg
      121 ggtagcggag gatctggacg tctccggggc ggtcgatccg ttcgaccgga agcgcagacc
      181 gaacctggcc cggtggctag cgttcgagga gcaaccgttt gacgtgatcg tggcgtaccg
      241 ggtagatcgg ttgacccgat cgatccggca tcttcagcag ctggtccact gggccgagga
      301 ccacaagaag ctggtcgtct ccgcgaccga agcgcacttc gatacgacga cgccgtttgc
      361 ggcggtcgtc atcgcgctta tgggaacggt ggcgcagatg gaattagaag cgatcaaaga
      421 gcggaaccgt tcggctgcgc atttcaatat ccgcgccggg aaataccgag gatccctgcc
      481 gccgtgggga tacctgccta cgcgcgtgga cggggagtgg cggctggtgc cggaccctgt
      541 gcagcgagag cgcatcctcg aggtgtatca ccgcgtcgtc gacaaccacg agccgctgca
      601 tctggtggcc cacgacctga accggcgtgg tgtcctgtcg ccgaaggact acttcgcgca
      661 gctgcaaggc cgcgagccgc agggccggga gtggtcggct accgcgctga agcgatcgat
      721 gatctccggg gcgatgctcg ggtacgcgac tctgaacggt aagaccgtcc gagacgacga
      781 cggagccccg ctggtgcggg ctgagccgat cctgacccgt gagcagctgg aggcgctgcg
      841 cgccgagctc gtcaagacct cccgggcgaa gcccgcggtg tctaccccgt cgctgctgct
      901 gcgggtgttg ttctgcgcgg tgtgcgggga gcccgcgtac aagttcgccg ggggaggacg
      961 taagcacccg cgctaccgct gccgctcgat ggggttcccg aagcactgcg ggaacggcac
     1021 ggtggcgatg gccgagtggg acgcgttctg cgaggagcag gtactggatc tgctcgggga
     1081 cgcggagcgt ctggagaaag tctgggtagc gggctcggac tccgcggtcg aactcgcgga
     1141 ggtgaacgcg gagctggtgg acctgacgtc gctgatcggc tccccggcct accgggcggg
     1201 ctctccgcag cgagaagcac tggatgcccg tattgcggcg ctggccgcgc ggcaagagga
     1261 gctggagggc ctggaggctc gcccgtctgg ctgggagtgg cgcgaaaccg ggcagcggtt
     1321 cggggactgg tggcgggagc aggacaccgc ggcaaagaac acctggcttc ggtcgatgaa
     1381 cgttcggctg acgttcgacg tccgcggcgg gctgactcgc acgatcgact tcggggatct
     1441 tcaggagtac gagcagcatc tcaggctcgg cagcgtggtc gaacggctac acaccgggat
     1501 gtcgggtagc ggcagcggta gcggtagtgg gagcggcagc atggtcagta aaggcgaagc
     1561 agttatcaaa gagttcatgc gcttcaaagt tcatatggaa gggtcgatga acgggcacga
     1621 atttgaaatt gaaggcgaag gcgaaggccg cccatatgaa gggacccaaa ccgcaaagct
     1681 taaggttact aaaggcggtc cattaccctt ttcgtgggac attttaagcc cacagtttat
     1741 gtacgggagt cgcgctttca tcaagcaccc tgcggacatc ccagattact acaaacagtc
     1801 tttccccgag gggttcaagt gggagcgcgt gatgaacttc gaggatggcg gagccgtgac
     1861 ggtcacccaa gatacctctt tggaggacgg tacgttgatc tacaaagtga aattgcgtgg
     1921 cacgaatttt ccacctgatg ggcctgtcat gcagaaaaag acaatgggat gggaagcttc
     1981 cacggagcgc ctttacccag aggacggtgt tcttaaaggg gatatcaaaa tggcgctgcg
     2041 tcttaaagat ggaggccgct acctggcgga cttcaagact acttacaagg ccaaaaaacc
     2101 agtgcagatg ccgggtgcgt acaatgtaga tcgtaaatta gatattacaa gtcacaatga
     2161 agattacacg gtcgtagagc agtatgagcg cagtgagggg cgtcactcta cgggcggtat
     2221 ggacgagtta tacaaggcgg ctaacgacga gaattatgca ttggccgcct aataaaggta
     2281 gagacctact agtagcggcc gctgcaggct tcctcgctca ctgactcgct gcgctcggtc
     2341 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2401 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2461 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
     2521 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2581 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2641 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2701 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2761 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2821 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2881 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2941 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3001 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3061 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3121 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3181 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     3241 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     3301 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     3361 cccagtgctg caatgatacc gcgcgaccca cgctcaccgg ctccagattt atcagcaata
     3421 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     3481 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     3541 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3601 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3661 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     3721 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3781 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3841 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3901 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3961 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     4021 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     4081 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     4141 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     4201 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     4261 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta
     4321 gctttcgcta aggatgattt ctggaattcg cggccgcttc tagagactag tgggtctca
//
