LOCUS       sequence-231089-        2836 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for RpaR-TYQEVV (Adam Meyer's 
            improved RpaR activator selected for dynamic range and low 
            cross-sensitivity using methods from 
            https://doi.org/10.1101/285866,)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2836)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C70m
FEATURES             Location/Qualifiers
     source          1..2836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(154..945,946..1014))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     777..796
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1015..1119)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1187..1205
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1209..1252)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1255..1276
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    2034..2054
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(2060..2077)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2231..2819)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2311..2330)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
       61 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      121 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
      181 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
      241 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgcgaccc
      301 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
      361 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
      421 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
      481 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
      541 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
      601 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
      661 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
      721 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
      781 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
      841 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
      901 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
      961 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1021 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1081 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1141 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     1201 gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc
     1261 gcggccgctt ctagagacta gtgggtctca aatgattgtg ggtgaagatc agctgtgggg
     1321 tcgtcgtaca ctggaatttg ttgatagcgt tgaacgtctg gaagcaccgg cactgattag
     1381 ccgttttgaa agcctgattg caagctgtgg ttttaccgcc tatatcatgg caggtctgcc
     1441 gagccgtaat gccggtctgc cggaactgac cctggcaaat ggttggcctc gtgattggtt
     1501 tgatctgtat gttagcgaaa actttagcgc agttgatccg gttccgcgtt atggtgcaac
     1561 cactgttcat ccgtttgttt ggagtgatgc accgtatgat cgtgaccgtg atcaggcagc
     1621 acatcgtgtt atgacccgtg cagcagaatt tggtctggtt gaaggttatt gtattccgct
     1681 gcattacgat gatggtagcg cagcaattag tatggcaggt gaagatcctg atctgagtcc
     1741 ggcagcccgt ggtgtaatgc agctggttag catttatgca catagccgtc tgcgtgtact
     1801 gagccgtccg aaaccgattc gtcgtaatcg tctgacaccg cgtgaatgtg aaattctgca
     1861 gtgggcagca cagggtaaaa ccgcatggga aattagcgtt attctgtgta ttaccgaacg
     1921 caccgttaaa tttcatctga ttgaagcagc acgtaaactg gatgcagcaa atcgtaccgc
     1981 agcagttgca aaagcactga cactgggtct gattcgtctg tgaggtagag acctactagt
     2041 agcggccgct gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     2101 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     2161 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     2221 tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2281 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2341 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2401 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     2461 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2521 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2581 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2641 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     2701 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2761 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2821 aagatccttt gatctt
//
