LOCUS       sequence-231095-        2924 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for KanR (kanamycin resistance 
            protein). Please see Supplemental Documents for annotated Genbank 
            file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2924)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C76m
FEATURES             Location/Qualifiers
     source          1..2924
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             5..820
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(78..97)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    832..852
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(858..875)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1029..1617)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1109..1128)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1788..2579,2580..2648))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2411..2430
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2649..2753)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2821..2839
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2843..2886)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2889..2910
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatgatgagc catattcaac gggaaacgtc ttgctcccgt ccgcgcttaa actccaacat
       61 ggacgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
      121 aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
      181 tagcgttgcc aatgatgtta cagatgagat ggtccgtctc aactggctga cggagtttat
      241 gcctctcccg accatcaagc attttatccg tactcctgat gatgcgtggt tactcaccac
      301 cgcgattcct gggaaaacag ccttccaggt attagaagaa tatcctgatt caggtgaaaa
      361 tattgttgat gcgctggccg tgttcctgcg ccggttacat tcgattcctg tttgtaattg
      421 tccttttaac agcgatcgtg tatttcgtct tgctcaggcg caatcacgca tgaataacgg
      481 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
      541 gaaagaaatg cacaagctct tgccattctc accggattca gtcgtcactc atggtgattt
      601 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg
      661 ggtcggaatc gcagaccgtt accaggacct tgccattctt tggaactgcc tcggtgagtt
      721 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
      781 taaattgcag tttcatttga tgctcgatga gtttttctaa aggtagagac ctactagtag
      841 cggccgctgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      901 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      961 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1021 ctggcgtttt tccacaggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1081 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1141 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1201 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1261 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1321 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1381 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1441 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1501 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1561 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1621 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1681 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1741 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     1801 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     1861 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     1921 ataccgcgcg acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     1981 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     2041 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     2101 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2161 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2221 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2281 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2341 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2401 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     2461 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2521 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2581 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2641 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     2701 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     2761 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     2821 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
     2881 gatttctgga attcgcggcc gcttctagag actagtgggt ctca
//
