LOCUS       sequence-231099-        2327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for ccdA (Antitoxin to ccdB/C39m.
            Sequence taken from pMAR7 plasmid sequence). Please see Supplemental
            Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2327)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C79m
FEATURES             Location/Qualifiers
     source          1..2327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    235..255
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(261..278)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(432..1020)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(512..531)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1191..1982,1983..2051))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1814..1833
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2052..2156)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2224..2242
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2246..2289)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2292..2313
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatgatgaag cagcgtatta cagtgacagt tgacagcgac agctatcaat tgctcaaggc
       61 atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct
      121 gcgtgccgaa cgctggaaag tggaaaatca ggaagggatg gttgaggtcg cccggtttat
      181 tgaaatgaac ggctcttttg ccgatgagaa cagggactgg tgaaggtaga gacctactag
      241 tagcggccgc tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      301 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      361 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      421 ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      481 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      541 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      601 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
      661 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
      721 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
      781 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
      841 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
      901 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
      961 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1021 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     1081 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     1141 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     1201 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     1261 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     1321 atgataccgc gcgacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     1381 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     1441 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     1501 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     1561 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     1621 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     1681 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     1741 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     1801 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     1861 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     1921 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     1981 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     2041 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     2101 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     2161 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     2221 aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag
     2281 gatgatttct ggaattcgcg gccgcttcta gagactagtg ggtctca
//
