LOCUS       sequence-231100-        4469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for mfLon protease (see doi: 
            [10.1038/nbt.3053]). Please see Supplemental Documents for annotated
            Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4469)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C80m
FEATURES             Location/Qualifiers
     source          1..4469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(14..31)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(185..773)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(265..284)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(944..1735,1736..1804))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1567..1586
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1805..1909)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1977..1995
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1999..2042)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2045..2066
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    join(4457..4469,1..8)
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 cgctgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
       61 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
      121 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      181 cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      241 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
      301 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
      361 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
      421 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
      481 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
      541 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
      601 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
      661 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
      721 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
      781 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
      841 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
      901 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
      961 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     1021 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     1081 cgcgcgaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     1141 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     1201 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     1261 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     1321 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     1381 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     1441 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     1501 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     1561 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     1621 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     1681 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     1741 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     1801 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     1861 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     1921 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     1981 ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt
     2041 tctggaattc gcggccgctt ctagagacta gtgggtctca aatgtctaaa aaaatcaaac
     2101 tgccgatctt ccagatccgt ggttctttca tcgttccggg tatcaaagaa aacctggaag
     2161 ttggtcgtaa aaacaccctg gcgtctgtta actacgcgat caaaaactct aacaaccaga
     2221 tgatcgcgat cccgcagatc gacgcgtctg ttgaaaaacc ggaattctct gacctgcacg
     2281 aattcggtat cctgatcgac ttcgaagtta tcaaagaatg gaaagacaac tctctgacca
     2341 tctctaccaa cccgatccag cgttgcaaag ttatctcttt cttcgaaaac gaagatcagg
     2401 ttccgtacgc ggaagttgaa ctgatcgaat ctatcaacga cttctctgac gaagaactga
     2461 aagaactgat cgaaaaaatc tctgacgcga tcaaaaccaa agcgtctctg gttaccaaac
     2521 agatcaaaca gctgatctct ggtgaatctg acgacctgtc tctggcgttc gactctatca
     2581 tgttcaaact ggcgccgtct aaaatcctga ccaacccgga atacatcacc tctccgtctc
     2641 tgaaaacccg ttggtctatc atcgaaaaaa tcatcttcgc ggaagatggt atcatcaccc
     2701 gtaacgcgga atctatcgac gcggcgcgtc agaaaaacga aatcgaacag gaactgaacc
     2761 acaaactgaa agaaaaaatg gacaaacagc agaaagaata ctacctgcgt gaaaaaatgc
     2821 gtatcatcaa agacgaactg gaagatgaag atgactctga cgactcttct ctggaaaaat
     2881 acaaagaacg tctggcgaaa gaaccgttcc cggaagaagt taaacgtaaa atcatggcgt
     2941 ctatcaaacg tgttgaagcg ctgcagtctg gtaccccgga atggaacacc gaaaaaaact
     3001 acatcgactg gatgatgtct atcccgtggt gggaagaaac cgaagatctg accgacctga
     3061 aatacgcgaa aaaaatcctg gacaaacacc actacggtat gaaaaaagtt aaagaacgta
     3121 tcatcgaata cctggcggtt aaaaccaaaa ccaaatctct gaaagcgccg atcatcaccc
     3181 tggttggtcc gccgggtgtt ggtaaaacct ctctggcgaa atctatcgcg gaagcggttg
     3241 gtaaaaactt cgttaaagtt tctctgggtg gtgttaaaga cgaatctgaa atccgtggtc
     3301 accgtaaaac ctacgttggt tctatgccgg gtcgtatcat ccagaccatg aaacgtgcga
     3361 aagttaaaaa cccgctgttc ctgctggacg aaatcgacaa aatggcgtct gaccaccgtg
     3421 gtgacccggc gtctgcgatg ctggaagttc tggacccgga acagaacaaa gaattctctg
     3481 accactacat cgaagaaccg tacgacctgt ctcaggttat gttcatcgcg accgcgaact
     3541 acccggaaga tatcccggaa gcgctgtacg accgtatgga aatcatcaac ctgtcctctt
     3601 acaccgaaat cgaaaaagtt aaaatcgcgc aggactacct ggttccgaaa gcgatcgaac
     3661 agcacgaact gacctctgaa gaaatctctt tcaccgaagg tgcgatcaac gaaatcatca
     3721 aatactacac ccgtgaagcg ggtgttcgtc agctggaacg tcacatcaac tctatcatcc
     3781 gtaaatacat cgttaaaaac ctgaacggtg aaatggacaa aatcgttatc gacgaaaaac
     3841 aggttaacga cctgctgggt aaacgtatct tcgaccacac cgaaaaacag gaagaatctc
     3901 agatcggtgt tgttaccggt ctggcgtaca cccagttcgg tggtgacatc ctgccgatcg
     3961 aagtttctct gtacccgggt aaaggtaacc tgatcctgac cggtaaactg ggtgaagtta
     4021 tgaaagaatc tgcgaccatc gcgctgacct acgttaaatc taacttcgaa aaattcggtg
     4081 ttgacaaaaa agttttcgaa gaaaacgaca tccacgttca cgttccggaa ggtgcggttc
     4141 cgaaagacgg tccgtctgcg ggtatcacca tcaccaccgc gctgatctct gcgctgtctg
     4201 acaaaccggt ttctaaagaa atcggtatga ccggtgaaat caccctgcgt ggtaacgttc
     4261 tgccgatcgg tggtctgcgt gaaaaatcta tctctgcgtc tcgttctggt ctgaaaacca
     4321 tcatcatccc gaaaaaaaac gaacgtgacc tggacgaaat cccggacgaa gttaaagcga
     4381 aactgaaaat catcccggcg gaaaaatacg aagaagtttt cgcgatcgtt ttcaaaacca
     4441 aataaaggta gagacctact agtagcggc
//
