LOCUS       sequence-231114-        2303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for Gp2 (RNA polymerase inhibitor
            from T7 phage; lethal to host when expressed). Please see 
            Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2303)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C98m
FEATURES             Location/Qualifiers
     source          1..2303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    211..231
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(237..254)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(408..996)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(488..507)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1167..1958,1959..2027))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1790..1809
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2028..2132)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2200..2218
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2222..2265)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2268..2289
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatgatgtca aacgtaaata caggttcact tagtgtggac aataagaagt tttgggctac
       61 cgtagagtcc tcggagcatt ccttcgaggt tccaatctac gctgagacac tagacgaagc
      121 tctggagtta gccgaatggc aatacgttcc ggctggcttt gaggttactc gtgtgcgtcc
      181 ttgtgtagca ccgaagtaaa ggtagagacc tactagtagc ggccgctgca ggcttcctcg
      241 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
      301 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
      361 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc
      421 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      481 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      541 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      601 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
      661 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
      721 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
      781 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
      841 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
      901 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
      961 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1021 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     1081 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     1141 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     1201 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     1261 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgcga cccacgctca
     1321 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     1381 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1441 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1501 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1561 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     1621 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1681 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1741 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1801 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1861 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1921 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1981 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     2041 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     2101 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     2161 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag
     2221 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg
     2281 cttctagaga ctagtgggtc tca
//
