LOCUS       sequence-231116-        2906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part sfYFP-ptd3 (superfolder YFP with 
            pdt3 degradation tag for mfLon). Please see Supplemental Documents 
            for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2906)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C100m
FEATURES             Location/Qualifiers
     source          1..2906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             5..718
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    814..834
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(840..857)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1011..1599)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1091..1110)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1770..2561,2562..2630))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2393..2412
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2631..2735)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2803..2821
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2825..2868)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2871..2892
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatgatgcgt aaaggcgaag agctgttcac tggtgtcgtc cctattctgg tggaactgga
       61 tggtgatgtc aacggtcata agttttccgt gcgtggcgag ggtgaaggtg acgcaactaa
      121 tggtaaactg acgctgaagt tcatctgtac tactggtaaa ctgccggtac cttggccgac
      181 tctggtaacg acgctgactt atggtgttca gtgctttgct cgttatccgg accatatgaa
      241 gcagcatgac ttcttcaagt ccgccatgcc ggaaggctat gtgcaggaac gcacgatttc
      301 ctttaaggat gacggcacgt acaaaacgcg tgcggaagtg aaatttgaag gcgataccct
      361 ggtaaaccgc attgagctga aaggcattga ctttaaagaa gacggcaata tcctgggcca
      421 taagctggaa tacaatttta acagccacaa tgtttacatc accgccgata aacaaaaaaa
      481 tggcattaaa gcgaatttta aaattcgcca caacgtggag gatggcagcg tgcagctggc
      541 tgatcactac cagcaaaaca ctccaatcgg tgatggtcct gttctgctgc cagacaatca
      601 ctatctgagc taccaaagcg ttctgtctaa agatccgaac gagaaacgcg atcatatggt
      661 tctgctggag ttcgtaaccg cagcgggcat cacgcatggt atggatgaac tgtacaaagc
      721 ggcgaacaaa aacgaagaaa acaccaacga agtgccgacc tttatgctga acgcgggcca
      781 ggcgaacaga agacgagttt aaaggtagag acctactagt agcggccgct gcaggcttcc
      841 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      901 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
      961 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg
     1021 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1081 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1141 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1201 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1261 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1321 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1381 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1441 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1501 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1561 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1621 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1681 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1741 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     1801 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     1861 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg cgacccacgc
     1921 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     1981 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     2041 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     2101 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     2161 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     2221 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     2281 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     2341 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     2401 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     2461 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     2521 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     2581 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     2641 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     2701 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     2761 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     2821 cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcgcgg
     2881 ccgcttctag agactagtgg gtctca
//
