LOCUS       sequence-231118-        3092 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part treRT-LAA (synthetic Lac-family 
            repressor chimera with LAA degradation tag for ssrA; see DOI: 
            10.1021/sb500262f)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3092)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C102m
FEATURES             Location/Qualifiers
     source          1..3092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..43)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     misc_feature    1000..1020
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(1026..1043)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1197..1785)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1277..1296)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1956..2747,2748..2816))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2579..2598
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2817..2921)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2989..3007
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3011..3054)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3057..3078
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatgatgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct ctacggcgac
       61 cgtttccaac gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga
      121 agcggcgatg gcggagctca attacattcc caaccgcgcc gcacaacaac tggcgggcaa
      181 acagagcaaa gtggtcgcca tcattgttac ccgtctggat tcgttgtcag aaaatctcgc
      241 cgttcaaacc atgctgccag cgttctatga acaaggttac gacccaatca tgatggaaag
      301 tcagttttcc ccgcaattag ttgccgaaca tttgggagtg ctgaaacggc gtaatatcga
      361 cggcgtagtg ctgttcggtt ttactggcat aacagaagaa atgttagccc actggcagtc
      421 atcgctggtt ctgctggcgc gtgacgcaaa aggctttgct tcggtctgtt atgacgacga
      481 aggggcaatc aaaatcctga tgcaacggct gtatgaccag gggcatcgta atatcagtta
      541 tctcggcgtg ccgcacagtg acgtgacaac cggtaagcga cgtcacgaag cctacctggc
      601 gttctgcaaa gcgcataaac tgcatcccgt tgccgccctg ccagggcttg ctatgaagca
      661 aggctatgag aacgttgcaa aagtgattac gcctgaaact accgccttac tgtgcgcaac
      721 cgacacgctg gcacttggcg caagtaaata cctgcaagag caacgcatcg acaccttgca
      781 actggcgagc gtcggtaata cgccgttaat gaaattcctc catccggaga tcgtaaccgt
      841 agatcccggt tacgccgaag ctggacgcca ggcggcttgc cagttgatcg cgcaggtaac
      901 cgggcgcagc gaaccgcaac aaatcatcat ccccgccacc ctgtccacta gtgcagcgaa
      961 cgacgaaaat tacgcccttg cagcgtgaag gtagagacct actagtagcg gccgctgcag
     1021 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     1081 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     1141 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     1201 cacaggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     1261 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1321 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1381 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1441 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1501 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1561 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     1621 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1681 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1741 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1801 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1861 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1921 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1981 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     2041 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgcgac
     2101 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     2161 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     2221 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     2281 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     2341 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     2401 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     2461 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     2521 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     2581 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     2641 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     2701 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     2761 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     2821 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     2881 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     2941 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     3001 acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat
     3061 tcgcggccgc ttctagagac tagtgggtct ca
//
