LOCUS       sequence-23114-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Polyak K, Xia Y, Zweier JL, Kinzler KW, Vogelstein B
  TITLE     A model for p53-induced apoptosis.
  JOURNAL   Nature. 1997 Sep 18. 389(6648):300-5.
  PUBMED    9305847
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1997 Sep 18. 389(6648):300-5."
COMMENT     Sequence Label: pBK-CMV Pig3
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    114..130
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     119..141
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     138..154
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     138..154
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        175..193
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agccgcaatt actgtgagtt agctcactca
       61 ttaggcaccc caggctttac actttatact tccggctcgt atattgtgtg gaattgtgag
      121 cggataacaa tttcacacag gaaacagcta tgaccttgat tacgccaagc tcgaaattaa
      181 ccctcactaa agggaacaaa agctggagct cgcgcgcctg caggtcgaca ctagtggatc
      241 caaagaattc ggcacgagcc ggtggaggca gaggcagtcc tggggctctg gggctcgggc
      301 tttgtcaccg ggacccgcag gagccagaac cactcggcgc cgcctggtgc atgggagggg
      361 agccgggcca ggagtaagta actcatacgg gcgccgggga cccgggtcgg gctgggggct
      421 tccaactcag agggagtgtg atttgcctga tcctcttcgg cgttgtcctg ctctgccgca
      481 tccagccctg taccgccatc ccacttcccg ccgttcccat ctgtgttccg ggtgggatcg
      541 gtctggaggc ggccgaggac ttcccaggca ggagctcggg gcggaggccg ggtccgcggc
      601 agaccagggc agcgaggcgc tggccggcag ggnnnnnggg ggnncnnnnc nnnnnnntgg
      661 gcnnnnnccg cgaggataca gcggccccct gcccctgtcc tgtcctgccc tgccctgtcc
      721 ctgtcctgcc ctgccctgcc ctgtcctgtc ctgccctgcc ctgccctgtg tcctcagaca
      781 atatgttagn cc
//
