LOCUS       sequence-231142-        2267 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly BC part for RiboJ-BCD2 (low expression 
            bi-cistronic RBS, engineered for context-independence, prefixed by 
            self-cleaving RiboJ; see https://doi.org/10.1038/nmeth.2404)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2267)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: U24m
FEATURES             Location/Qualifiers
     source          1..2267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..144
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(148..191)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    194..215
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_RNA        236..285
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             379..387
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    404..424
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(430..447)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(601..1189)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(681..700)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1360..2151,2152..2220))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1983..2002
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(2221..2267,1..58))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
       61 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
      121 ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta
      181 aggatgattt ctggaattcg cggccgcttc tagagactag tgggtctcat actagctgtc
      241 accggatgtg ctttccggtc tgatgagtcc gtgaggacga aacagcctct acaaataatt
      301 ttgtttaagg gcccaagttc acttaaaaag gagatcaaca atgaaagcaa ttttcgtact
      361 gaaacatctt aatcatgcta aggaggtttt ctaatgagag acctactagt agcggccgct
      421 gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
      481 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
      541 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      601 tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      661 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      721 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      781 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      841 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      901 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      961 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1021 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1081 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1141 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1201 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1261 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1321 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     1381 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     1441 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     1501 cgacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     1561 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1621 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1681 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1741 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1801 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1861 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1921 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1981 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     2041 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     2101 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     2161 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     2221 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtc
//
