LOCUS       sequence-231153-        2170 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly DE part for L3S2P55 (256x-strength 
            synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see 
            Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2170)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T16m
FEATURES             Location/Qualifiers
     source          1..2170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    78..98
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(104..121)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(275..863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(355..374)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1034..1825,1826..1894))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1657..1676
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1895..1999)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2067..2085
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2089..2132)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2135..2156
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aggtacggtc ggtaccaaag acgaacaata agacgctgaa aagcgtcttt tttcgttttg
       61 gtcccggctt agagacctac tagtagcggc cgctgcaggc ttcctcgctc actgactcgc
      121 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
      181 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
      241 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca caggctccgc ccccctgacg
      301 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      361 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      421 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      481 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      541 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      601 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      661 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
      721 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      781 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      841 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      901 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      961 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     1021 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     1081 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     1141 taccatctgg ccccagtgct gcaatgatac cgcgcgaccc acgctcaccg gctccagatt
     1201 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1261 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1321 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1381 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1441 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1501 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1561 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1621 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1681 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1741 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1801 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1861 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1921 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1981 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     2041 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa
     2101 aaaaatcctt agctttcgct aaggatgatt tctggaattc gcggccgctt ctagagacta
     2161 gtgggtctca
//
