LOCUS       sequence-231169-        4947 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Monomeric near-infrared fluorescent protein miRFP670 in bacterial 
            plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Shcherbakova DM, Baloban M, Emelyanov AV, Brenowitz M, Guo P, 
            Verkhusha VV
  TITLE     Bright monomeric near-infrared fluorescent proteins as tags and 
            biosensors for multiscale imaging.
  JOURNAL   Nat Commun. 2016 Aug 19;7:12405. doi: 10.1038/ncomms12405.
  PUBMED    27539380
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-08-19"; volume: "7:12405. doi"; pages: " 
            10.1038/ncomms12405"
COMMENT     Sequence Label: pBAD/His-miRFP670
FEATURES             Location/Qualifiers
     source          1..4947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             55..72
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..1029
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 670 nm (Shcherbakova et al., 2016)"
                     /label=miRFP670
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     primer_bind     complement(1075..1092)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1245..1331
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1423..1450
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1469..1560
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1561..1629,1630..2421)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1779..1798)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2592..3180
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3081..3100
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3334..3351
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3366..3506
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3432..3451)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3592..3614
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3770..4648)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        join(4675..4947,1..12)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     4882..4901
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 ctgtttctcc atacccgttt tttgggctaa caggaggaat taaccatggg gagccaccat
       61 caccatcacc atggcagatc tatggtagca ggtcatgcct ctggcagccc cgcattcggg
      121 accgcctctc attcgaattg cgaacatgaa gagatccacc tcgccggctc gatccagccg
      181 catggcgcgc ttctggtcgt cagcgaacat gatcatcgcg tcatccaggc cagcgccaac
      241 gccgcggaat ttctgaatct cggaagcgta ctcggcgttc cgctcgccga gatcgacggc
      301 gatctgttga tcaagatcct gccgcatctc gatcccaccg ccgaaggcat gccggtcgcg
      361 gtgcgctgcc ggatcggcaa tccctctacg gagtactgcg gtctgatgca tcggcctccg
      421 gaaggcgggc tgatcatcga actcgaacgt gccggcccgt cgatcgatct gtcaggcacg
      481 ctggcgccgg cgctggagcg gatccgcacg gcgggttcac tgcgcgcgct gtgcgatgac
      541 accgtgctgc tgtttcagca gtgcaccggc tacgaccggg tgatggtgta tcgtttcgat
      601 gagcaaggcc acggcctggt attctccgag tgccatgtgc ctgggctcga atcctatttc
      661 ggcaaccgct atccgtcgtc gactgtcccg cagatggcgc ggcagctgta cgtgcggcag
      721 cgcgtccgcg tgctggtcga cgtcacctat cagccggtgc cgctggagcc gcggctgtcg
      781 ccgctgaccg ggcgcgatct cgacatgtcg ggctgcttcc tgcgctcgat gtcgccgtgc
      841 catctgcagt tcctgaagga catgggcgtg cgcgccaccc tggcggtgtc gctggtggtc
      901 ggcggcaagc tgtggggcct ggttgtctgt caccattatc tgccgcgctt catccgtttc
      961 gagctgcggg cgatctgcaa acggctcgcc gaaaggatcg cgacgcggat caccgcgctt
     1021 gagagctaag aattcgaagc ttggctgttt tggtggatga gagaagattt tcagcctgat
     1081 acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag
     1141 cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg
     1201 tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg
     1261 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
     1321 gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc
     1381 gggcaggacg cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg
     1441 atggcctttt tgcgtttcta caaactcttt tgtttatttt tctaaataca ttcaaatatg
     1501 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     1561 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     1621 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     1681 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     1741 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     1801 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     1861 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     1921 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     1981 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     2041 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     2101 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     2161 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     2221 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     2281 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     2341 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     2401 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     2461 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     2521 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     2581 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2641 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2701 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     2761 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2821 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2881 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2941 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3001 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3061 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3121 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3181 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3241 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3301 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3361 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     3421 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     3481 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     3541 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     3601 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagcag atcaattcgc
     3661 gcgcgaaggc gaagcggcat gcataatgtg cctgtcaaat ggacgaagca gggattctgc
     3721 aaaccctatg ctactccgtc aagccgtcaa ttgtctgatt cgttaccaat tatgacaact
     3781 tgacggctac atcattcact ttttcttcac aaccggcacg gaactcgctc gggctggccc
     3841 cggtgcattt tttaaatacc cgcgagaaat agagttgatc gtcaaaacca acattgcgac
     3901 cgacggtggc gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt
     3961 ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg gcggaaaaga tgtgacagac
     4021 gcgacggcga caagcaaaca tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca
     4081 ggtgatcgct gatgtactga caagcctcgc gtacccgatt atccatcggt ggatggagcg
     4141 actcgttaat cgcttccatg cgccgcagta acaattgctc aagcagattt atcgccagca
     4201 gctccgaata gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga
     4261 aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt attggcaaat attgacggcc
     4321 agttaagcca ttcatgccag taggcgcgcg gacgaaagta aacccactgg tgataccatt
     4381 cgcgagcctc cggatgacga ccgtagtgat gaatctctcc tggcgggaac agcaaaatat
     4441 cacccggtcg gcaaacaaat tctcgtccct gatttttcac caccccctga ccgcgaatgg
     4501 tgagattgag aatataacct ttcattccca gcggtcggtc gataaaaaaa tcgagataac
     4561 cgttggcctc aatcggcgtt aaacccgcca ccagatgggc attaaacgag tatcccggca
     4621 gcaggggatc attttgcgct tcagccatac ttttcatact cccgccattc agagaagaaa
     4681 ccaattgtcc atattgcatc agacattgcc gtcactgcgt cttttactgg ctcttctcgc
     4741 taaccaaacc ggtaaccccg cttattaaaa gcattctgta acaaagcggg accaaagcca
     4801 tgacaaaaac gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt
     4861 tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta gcggatccta
     4921 cctgacgctt tttatcgcaa ctctcta
//
