LOCUS       sequence-23121-e         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Polyak K, Xia Y, Zweier JL, Kinzler KW, Vogelstein B
  TITLE     A model for p53-induced apoptosis.
  JOURNAL   Nature. 1997 Sep 18. 389(6648):300-5.
  PUBMED    9305847
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1997 Sep 18. 389(6648):300-5."
COMMENT     Sequence Label: pBK-CMV Pig12
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    117..133
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     122..144
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     141..157
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     141..157
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        178..196
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gcngagctgg tttagtgaac cgtcagatcc gctagccgca attactgtga gttagctcac
       61 tcattaggca ccccaggctt tacactttat acttccggct cgtatattgt gtggaattgt
      121 gagcggataa caatttcaca caggaaacag ctatgacctt gattacgcca agctcgaaat
      181 taaccctcac taaagggaac aaaagctgga gctcgcgcgc ctgcaggtcg acactagtgg
      241 atccaaagaa ttcggcacga ggctggccag agatgcctgc ccacagcctg gtgatgagca
      301 gcccggccct cccggccttc ctgctctgca gcacgctgct ggtcatcaag atgtacgtgg
      361 tggccatcat cacgggccaa gtgaggctgc ggaagaaggc ctttgccaac cccgaggatg
      421 ccctgagaca cggaggcccc cagtattgca ggagcgaccc cgacgtggaa cgctgcctca
      481 gggcccaccg gaacgacatg gagaccatct accccttcct tttcctgggc ttcgtctact
      541 cctttctggg tcctaaccct tttgtcgcct ggatgcactt cctggtcttc ctcgtgggcc
      601 gtgtggcaca caccgtggcc tacctgggga nngnnnggnn accnnnnnnc tccgtgacct
      661 acaccctggc ccagctcccc tgcgcctcca tggctctgca gatcctctgg gaagcggccc
      721 gccacctgtg accagcagct gatgcctcct tggccaccag accat
//
