LOCUS       sequence-231213-       10477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  hGem(1/110) fused with near-infrared fluorescent protein miRFP670v1 
            (S/G2/M phase cell cycle reporter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10477)
  AUTHORS   Shcherbakova DM, Baloban M, Emelyanov AV, Brenowitz M, Guo P, 
            Verkhusha VV
  TITLE     Bright monomeric near-infrared fluorescent proteins as tags and 
            biosensors for multiscale imaging.
  JOURNAL   Nat Commun. 2016 Aug 19;7:12405. doi: 10.1038/ncomms12405.
  PUBMED    27539380
REFERENCE   2  (bases 1 to 10477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-08-19"; volume: "7:12405. doi"; pages: " 
            10.1038/ncomms12405"
COMMENT     Sequence Label: pCSII-EF-miRFP670v1-hGem(1/110)
FEATURES             Location/Qualifiers
     source          1..10477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        162..541
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        543..741
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     696..716
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             759..939
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    986..1111
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1603..1836
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2021..2065
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2339..2456
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2512..3674
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2743..3665
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3622..3642
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3699..4643
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 670 nm (Shcherbakova et al., 2016)"
                     /label=miRFP670
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNSEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     CDS             4731..5060
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     misc_feature    5099..5687
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5152..5172)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5570..5581)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6206..6386
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6412..6429)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6418..6642
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6688..7116
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6775..6794)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6985..7006
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7125..7145)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7130..7459
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7310..7445
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7372..7391
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7507..7554
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7573..7947
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8077..8198
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8114..8133)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8168..8187
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8247..8263)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8247..8263)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8260..8282)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8271..8287
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8295..8301,8302..8319,8320..8325))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8340..8361
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8478..8495)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8649..9237)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8729..8748)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9408..10199,10200..10268))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10031..10050
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10269..10373)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10448..10467)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
       61 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
      121 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
      181 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      241 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      301 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      361 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      421 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      481 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      541 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      601 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      661 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      721 gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga
      781 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
      841 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
      901 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
      961 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     1021 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     1081 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     1141 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     1201 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     1261 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     1321 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     1381 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     1441 acagcaaggg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1501 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1561 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1621 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1681 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1741 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1801 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1861 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1921 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1981 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2041 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2101 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2161 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2221 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2281 cagagacaga tccattcgat tagtgaacgg atctacaaat ggcagtattc atccacaatt
     2341 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     2401 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     2461 tttattacag ggacagcaga aattcacttt gaattaattc aagcttcgtg aggctccggt
     2521 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     2581 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     2641 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     2701 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt
     2761 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc
     2821 tggctccagt acgtgattct tgatcccgag ctggagccag gggcgggcct tgcgctttag
     2881 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
     2941 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
     3001 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
     3061 aggatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
     3121 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
     3181 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
     3241 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
     3301 ttcccggccc tgctccaggg ggctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
     3361 gtgagtcacc cacacaaagg aaaggggcct ttccgtcctc agccgtcgct tcatgtgact
     3421 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctggagcttt tggagtacgt
     3481 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     3541 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     3601 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     3661 ttcaggtgtc gtgaacacgc taccggtctc gagaattcat ggtagcaggt catgcctctg
     3721 gcagccccgc attcgggacc gcctctcatt cgaatagcga acatgaagag atccacctcg
     3781 ccggctcgat ccagccgcat ggcgcgcttc tggtcgtcag cgaacatgat catcgcgtca
     3841 tccaggccag cgccaacgcc gcggaatttc tgaatctcgg aagcgtactc ggcgttccgc
     3901 tcgccgagat cgacggcgat ctgttgatca agatcctgcc gcatctcgat cccaccgccg
     3961 aaggcatgcc ggtcgcggtg cgctgccgga tcggcaatcc ctctacggag tactgcggtc
     4021 tgatgcatcg gcctccggaa ggcgggctga tcatcgaact cgaacgtgcc ggcccgtcga
     4081 tcgatctgtc aggcacgctg gcgccggcgc tggagcggat ccgcacggcg ggttcactgc
     4141 gcgcgctgtg cgatgacacc gtgctgctgt ttcagcagtg caccggctac gaccgggtga
     4201 tggtgtatcg tttcgatgag caaggccacg gcctggtatt ctccgagtgc catgtgcctg
     4261 ggctcgaatc ctatttcggc aaccgctatc cgtcgtcgac tgtcccgcag atggcgcggc
     4321 agctgtacgt gcggcagcgc gtccgcgtgc tggtcgacgt cacctatcag ccggtgccgc
     4381 tggagccgcg gctgtcgccg ctgaccgggc gcgatctcga catgtcgggc tgcttcctgc
     4441 gctcgatgtc gccgtgccat ctgcagttcc tgaaggacat gggcgtgcgc gccaccctgg
     4501 cggtgtcgct ggtggtcggc ggcaagctgt ggggcctggt tgtctgtcac cattatctgc
     4561 cgcgcttcat ccgtttcgag ctgcgggcga tctgcaaacg gctcgccgaa aggatcgcga
     4621 cgcggatcac cgcgcttgag agcggaggtg gtggatcagg tggaggaggt tctggaggtg
     4681 gtggatcagg aggaggtggt tctggaggtg gtggatcgcg gccgctcgag atgaatccca
     4741 gtatgaagca gaaacaagaa gaaatcaaag agaatataaa gaatagttct gtcccaagaa
     4801 gaactctgaa gatgattcag ccttctgcat ctggatctct tgttggaaga gaaaatgagc
     4861 tgtccgcagg cttgtccaaa aggaaacatc ggaatgacca cttaacatct acaacttcca
     4921 gccctggggt tattgtccca gaatctagtg aaaataaaaa tcttggagga gtcacccagg
     4981 agtcatttga tcttatgatt aaagaaaatc catcctctca gtattggaag gaagtggcag
     5041 aaaaacggag aaaggcgctg taatctagag ttaacatcga gggatcaagc ttatcgataa
     5101 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     5161 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     5221 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     5281 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     5341 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     5401 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     5461 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     5521 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     5581 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     5641 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     5701 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     5761 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     5821 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     5881 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     5941 ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata
     6001 tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga
     6061 agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga
     6121 cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc
     6181 ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct gagcctggga
     6241 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     6301 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     6361 ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg
     6421 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     6481 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     6541 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     6601 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     6661 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     6721 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     6781 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     6841 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     6901 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     6961 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     7021 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     7081 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     7141 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7201 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     7261 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     7321 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7381 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     7441 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     7501 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
     7561 aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
     7621 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
     7681 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
     7741 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
     7801 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
     7861 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
     7921 cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc
     7981 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     8041 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     8101 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     8161 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     8221 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     8281 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     8341 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     8401 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     8461 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     8521 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     8581 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8641 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8701 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8761 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8821 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8881 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8941 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     9001 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     9061 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     9121 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     9181 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     9241 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     9301 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     9361 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     9421 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     9481 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     9541 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     9601 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     9661 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     9721 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     9781 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9841 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     9901 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9961 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    10021 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    10081 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    10141 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    10201 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    10261 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    10321 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    10381 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
    10441 ctctcagtac aatctgctct gatgccgcat agttaag
//
