LOCUS       sequence-231216-        2866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_BR29_00026641
FEATURES             Location/Qualifiers
     source          1..2866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(690..706)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(690..706)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(703..725)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    714..730
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(738..744,745..762,763..768))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    783..804
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(921..938)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1092..1680)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1172..1191)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1851..2666)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1851..2666)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2574..2593
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(2667..2771)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2839..2857
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta agctcaaact ctaattggca taacaacaac tttaatttca tctggaaatt
      481 taccagacaa tttacaacct tcattaatag tagcttcata tttttgagaa ccattataag
      541 catgagccaa atatctagta attttatcac caacatcttg aactttttga acagaagaac
      601 catctggttg aaaataataa accaaacaag attcagtcat tagagaccaa gttccatccc
      661 aatggcgcgc cgagcttggc tcgagcatgg tcatagctgt ttcctgtgtg aaattgttat
      721 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
      781 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
      841 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
      901 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      961 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     1021 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     1081 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1141 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1201 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     1261 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     1321 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1381 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1441 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1501 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     1561 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1621 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1681 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     1741 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     1801 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttagaaaaac
     1861 tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt
     1921 tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca
     1981 agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc
     2041 ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt
     2101 gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc
     2161 tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg
     2221 agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg
     2281 cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat
     2341 acctggaatg ctgttttccc agggatcgca gtggtgagta accatgcatc atcaggagta
     2401 cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc
     2461 atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc
     2521 gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga
     2581 gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg cctagagcaa
     2641 gacgtttccc gttgaatatg gctcatactc ttcctttttc aatattattg aagcatttat
     2701 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     2761 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     2821 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
