LOCUS       sequence-23122-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Zawel L, Dai JL, Buckhaults P, Zhou S, Kinzler KW, Vogelstein B, 
            Kern SE
  TITLE     Human Smad3 and Smad4 are sequence-specific transcription 
            activators.
  JOURNAL   Mol Cell. 1998 Mar . 1(4):611-7.
  PUBMED    9660945
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            1998 Mar . 1(4):611-7."
COMMENT     Sequence Label: pET-SMAD4
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             25..47
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             66..83
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             93..110
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             129..161
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 gntgcggaca ttccctctga atattttgtt taactttaag aaggagatat accatgggca
       61 gcagccatca tcatcatcat cacagcagcg gcctggtgcc gcgcggcagc cgtcgtgcat
      121 ctgttcatat ggctagcatg actggtggac agcaaatggg tcgggatccg aattcgatgg
      181 acaatatgtc tattacgaat acaccaacaa gtaatggtgc ctgtctgagc attgtgcata
      241 gtttgatgtg ccatagacaa ggtggagaga gtgaaacatt tgcaaaaaga gcaattgaaa
      301 gtttggtaaa gaagctgaag gagaaaaaag atgaattgga ttctttaata acagctataa
      361 ctacaaatgg agctcatcct agtaaatgtg ttaccataca gagaacattg gatgggaggc
      421 ttcaggtggc tggtcggaaa ggatttcctc atgtgatcta tgcccgtctc tggaggtggc
      481 ctgatcttca caaaaatgaa ctaaaacatg ttaaatattg tcagtatgcg tttgacttaa
      541 aatgtgatag tgtctgtgtg aatccatatc actacgaacg agttgtatca cctggaattg
      601 atctctcagg attaacactg cagagtaatg ctccatcaag tatgatggtg aaggatgaat
      661 atgtgcatga ctttgaggga cagccatctg tgtccactga aggacattca attcaaacca
      721 tccagcatcc acaagtaatc gtgncatcgn caganacata cagcacccca gc
//
