LOCUS       sequence-23126-e         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Zawel L, Dai JL, Buckhaults P, Zhou S, Kinzler KW, Vogelstein B, 
            Kern SE
  TITLE     Human Smad3 and Smad4 are sequence-specific transcription 
            activators.
  JOURNAL   Mol Cell. 1998 Mar . 1(4):611-7.
  PUBMED    9660945
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            1998 Mar . 1(4):611-7."
COMMENT     Sequence Label: MBE6-Luc
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(31..227)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      45..180
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(340..356)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(341..357)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    381..472
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(413..432)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    486..534
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 ctttaccaac agtaccggaa agccaagctt tttgcaaaag cctaggcctc caaaaaagcc
       61 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa
      121 aaaaattagt cagcgatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
      181 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcagat cgcagatctc
      241 gagcccgggc tgcaggaatt cgatctagac atagtctaga caggtgtcta gacatagtct
      301 agacaggtgt ctagacatag tctagacaat caagcttatc gataccgtcg acctcgaggg
      361 ggggcccggt acctatcgat agagaaatgt tctggcacct gcacttgcac tggggacagc
      421 ctattttgct agtttgtttt gtttcgtttt gttttgatgg agagcgtatg ttagtactat
      481 cgattcacac aaaaaaccaa cacacagatg taatgaaaat aaagatattt tattgcggcc
      541 gctccaagta cctcccgtac cttaatatta cttacttatc atggtagctt gggctggcgt
      601 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
      661 tggcaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
      721 tcattttt
//
