LOCUS       sequence-23127-e         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Zawel L, Dai JL, Buckhaults P, Zhou S, Kinzler KW, Vogelstein B, 
            Kern SE
  TITLE     Human Smad3 and Smad4 are sequence-specific transcription 
            activators.
  JOURNAL   Mol Cell. 1998 Mar . 1(4):611-7.
  PUBMED    9660945
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            1998 Mar . 1(4):611-7."
COMMENT     Sequence Label: SBE2-Luc
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(33..229)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      47..182
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    311..402
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(343..362)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    416..464
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 ggctttacca acagtaccgg aatgccaagc tttttgcaaa agcctaggcc tccaaaaaag
       61 cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata
      121 aaaaaaatta gtcagcgatg gggcggagaa tgggcggaac tgggcggagt taggggcggg
      181 atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcag atcgcagatc
      241 tcgagcccgg gctagcacgc gtaagagctc ggtactaagt ctagacggca gtctagacgt
      301 acctatcgat agagaaatgt tctggcacct gcacttgcac tggggacagc ctattttgct
      361 agtttgtttt gtttcgtttt gttttgatgg agagcgtatg ttagtactat cgattcacac
      421 aaaaaaccaa cacacagatg taatgaaaat aaagatattt tattgcggcc gctccaagta
      481 cctcccgtac cttaatatta cttacttatc atggtagctt gggctggcgt aatagcgaag
      541 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcaaattg
      601 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
      661 accaataggc cgaaatcggc aaaatccctt ata
//
