LOCUS       sequence-23128-e         577 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Zawel L, Dai JL, Buckhaults P, Zhou S, Kinzler KW, Vogelstein B, 
            Kern SE
  TITLE     Human Smad3 and Smad4 are sequence-specific transcription 
            activators.
  JOURNAL   Mol Cell. 1998 Mar . 1(4):611-7.
  PUBMED    9660945
REFERENCE   2  (bases 1 to 577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            1998 Mar . 1(4):611-7."
COMMENT     Sequence Label: Vg4-Luc
FEATURES             Location/Qualifiers
     source          1..577
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(6..202)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      20..155
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    357..448
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(389..408)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    462..510
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gcttttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca
       61 gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcg atggggcgga
      121 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
      181 gttgctgact aattgagatg cagatcgcag atctcgagcc cgggctagca cgcgtaagag
      241 ctcggtacgc ttggctgccg tcgcgattcg tacgcttggc tgccgtcgcg attcgtacgc
      301 ttggctgccg tcgcgattcg tacgcttggc tgccgtcgcg attcgtacct atcgatagag
      361 aaatgttctg gcacctgcac ttgcactggg gacagcctat tttgctagtt tgttttgttt
      421 cgttttgttt tgatggagag cgtatgttag tactatcgat tcacacaaaa aaccaacaca
      481 cagatgtaat gaaaataaag atattttatt gcggccgctc caagtacctc ccgtacctta
      541 atattactta cttatcatgg tagcttgggc tggcgta
//
