LOCUS       sequence-23131-e         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Korinek V, Barker N, Morin PJ, van Wichen D, de Weger R, Kinzler KW,
            Vogelstein B, Clevers H
  TITLE     Constitutive transcriptional activation by a beta-catenin-Tcf 
            complex in APC-/- colon carcinoma.
  JOURNAL   Science. 1997 Mar 21. 275(5307):1784-7.
  PUBMED    9065401
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1997 Mar 21. 275(5307):1784-7."
COMMENT     Sequence Label: pcDNA/Myc TCF4
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..81
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           95..137
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     regulatory      159..168
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             168..197
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tggggcttta gcagagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa
       61 ttaatacgac tcactatagg gagacccaag cttgcttgtt ctttttgcag aagctcagaa
      121 taaacgctca actttggcag atctgatcta gaactagtgc cgccatggag cagaagctga
      181 tcagcgagga ggacctgcag cccggggatc cgagctcgaa ttccaccatg ccgcagctga
      241 acggcggtgg aggggatgac ctaggcgcca acgacgaact gatttccttc aaagacgagg
      301 gcgaacggga ggagaagagc tccgaaaact cctcggcaga gagggattta gctgatgtca
      361 aatcgtctct agtcaatgaa tcagaaacga atcaaaacag ctcctccgat tccgaggcgg
      421 aaagacggcc tccgcctcgc tccgaaagtt tccgagacaa atcccgggaa agtttggaag
      481 aagcggccaa gaggcaagat ggagggctct ttaaggggcc accgtatccc ggctacccct
      541 tcatcatgat ccccgacctg acgagcccct acctccccaa cggatcgctc tcgcccaccg
      601 cccgaaccta tctccagatg aaatggccac tgcttgatgt ccaggcaggg agcctccaga
      661 gtagacaagc cctcaaggat gcccggtccc catcaccggc acacattgtc tctaacaaag
      721 tg
//
