LOCUS       sequence-231315-        9403 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual luciferase assay for miRNA-targeted UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9403)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 9403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: pmiRGLO-GLIS2-201
FEATURES             Location/Qualifiers
     source          1..9403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        48..547
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(83..106)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     480..499
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      588..597
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             594..2246
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    4467..4588
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4478..4497)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4532..4551
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        4833..5190
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4833..5024
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4856..4876)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5041..5176
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      5235..5244
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5241..6173
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             6225..7016
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6276..6295)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6886..6905
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7080..7128
                     /note="synthetic polyadenylation signal"
     promoter        7284..7388
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7389..7457,7458..8249)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7607..7626)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(8407..8995)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8487..8506)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    9111..9394
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 cagtactccg gccacgatgc gtccggcgta gaggatcgag atctaccggg taggggaggc
       61 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
      121 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
      181 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
      241 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
      301 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
      361 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
      421 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
      481 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
      541 cctttcgacc tgcagcccaa gcttggcaat ccggtactgt tggtaaagcc accatggaag
      601 atgccaaaaa cattaagaag ggcccagcgc cattctaccc actcgaagac gggaccgccg
      661 gcgagcagct gcacaaagcc atgaagcgct acgccctggt gcccggcacc atcgccttta
      721 ccgacgcaca tatcgaggtg gacattacct acgccgagta cttcgagatg agcgttcggc
      781 tggcagaagc tatgaagcgc tatgggctga atacaaacca tcggatcgtg gtgtgcagcg
      841 agaatagctt gcagttcttc atgcccgtgt tgggtgccct gttcatcggt gtggctgtgg
      901 ccccagctaa cgacatctac aacgagcgcg agctgctgaa cagcatgggc atcagccagc
      961 ccaccgtcgt attcgtgagc aagaaagggc tgcaaaagat cctcaacgtg caaaagaagc
     1021 taccgatcat acaaaagatc atcatcatgg atagcaagac cgactaccag ggcttccaaa
     1081 gcatgtacac cttcgtgact tcccatttgc cacccggctt caacgagtac gacttcgtgc
     1141 ccgagagctt cgaccgggac aaaaccatcg ccctgatcat gaacagtagt ggcagtaccg
     1201 gattgcccaa gggcgtagcc ctaccgcacc gcaccgcttg tgtccgattc agtcatgccc
     1261 gcgaccccat cttcggcaac cagatcatcc ccgacaccgc tatcctcagc gtggtgccat
     1321 ttcaccacgg cttcggcatg ttcaccacgc tgggctactt gatctgcggc tttcgggtcg
     1381 tgctcatgta ccgcttcgag gaggagctat tcttgcgcag cttgcaagac tataagattc
     1441 aatctgccct gctggtgccc acactattta gcttcttcgc taagagcact ctcatcgaca
     1501 agtacgacct aagcaacttg cacgagatcg ccagcggcgg ggcgccgctc agcaaggagg
     1561 taggtgaggc cgtggccaaa cgcttccacc taccaggcat ccgccagggc tacggcctga
     1621 cagaaacaac cagcgccatt ctgatcaccc ccgaagggga cgacaagcct ggcgcagtag
     1681 gcaaggtggt gcccttcttc gaggctaagg tggtggactt ggacaccggt aagacactgg
     1741 gtgtgaacca gcgcggcgag ctgtgcgtcc gtggccccat gatcatgagc ggctacgtta
     1801 acaaccccga ggctacaaac gctctcatcg acaaggacgg ctggctgcac agcggcgaca
     1861 tcgcctactg ggacgaggac gagcacttct tcatcgtgga ccggctgaag agcctgatca
     1921 aatacaaggg ctaccaggta gccccagccg aactggagag catcctgctg caacacccca
     1981 acatcttcga cgccggggtc gccggcctgc ccgacgacga tgccggcgag ctgcccgccg
     2041 cagtcgtcgt gctggaacac ggtaaaacca tgaccgagaa ggagatcgtg gactatgtgg
     2101 ccagccaggt tacaaccgcc aagaagctgc gcggtggtgt tgtgttcgtg gacgaggtgc
     2161 ctaaaggact gaccggcaag ttggacgccc gcaagatccg cgagattctc attaaggcca
     2221 agaagggcgg caagatcgcc gtgtaattct agttgtttaa acgaggccca tcctgcggac
     2281 agttgtggtg cccccccggc agctcccggc actgcccccg acgaacggaa actcttctgt
     2341 gaaatagcaa taatgtccta ctgcccgggc agccccagcc cagcccgccg ggagcaagga
     2401 tggtgctagg tcattcatgg ctggcctccc agcccccggg tggggacctg gcctgtcatg
     2461 cagggagagc tgtgctcctg ggtgctgaag cctcgctcct gtctgtcccc caccacctgg
     2521 ccctcagctt ctgagaggct ttcccctgcc cgacctcctc ccgtttccct ctcccaccct
     2581 ggcacctccc tcacctagtg accacccatg gcaagttgcc ctctcccagc agagggggtg
     2641 ggtggggtgg catctgccct ccctgctagc accaggctcc cccttcctga gaggagcccc
     2701 cagggaccag aggcctgccc ttccctccta ggcttaccca gcccctgccc tgggggctcc
     2761 ttggacccct ttccctctga ccctgcctcc agagggaaag caagacagat gcaggcccct
     2821 gcaaagcccc aggtagaagc atgcccccca ggacaaggcg cctcccacta gttaggagga
     2881 ggcccgctct gcagccgccg tcctcacccc aggccaggcc tgcagtacca gacgggatag
     2941 ctggccactc cacccctgca ccccagggtc tcctccctct accttttggg gcaccctggg
     3001 agcgtgggaa gcaggtccga gggcccctga gctggcaagg ggaggtgcca ggccagctgt
     3061 ggtgccaaga tactgagtga cctgggccct ggctcaggga gcatgtgggg ccaggcccag
     3121 cgccccgtct tcctccttct acccccgctg ggcctggcct gggcagcgcc ccctgcagag
     3181 gcctttgggt ccttggtcct gtaacaggaa gggggaggct ggctggggac gaccgaccac
     3241 aggctgggac acagctcctg gtctgggggc tccaagtgac agcatgcagg ggagggggct
     3301 cccagtcagt gctgtgttgg gagctttctg gaggctgtgg actgaaggcc ttgagggaag
     3361 cagtggctgg aggagggtgc tggacccatg acacgttgct tcctctggct tttccctgct
     3421 gggccgcttt ctcagaggca cttccccacc cctaacaccc agtgggcccc cccaggttct
     3481 gtgccactca gagggaccct ggcaggggcc agaaccactt aagggtggtg ctggagggcc
     3541 ttgtgcccca gtcccatccc aggacgccct gagggatgga cgcagccatg caccccccat
     3601 ctggggcctc tccctgctcc ctctcccacc tggcagctgg gagttctggc ttctaggcct
     3661 gccctgtcac caggcctctg agtggccagg cccttccacc tccccatctg taaaacgagg
     3721 cagctgcccg gacagccttg gggtccttag tggccctgca ggtcctctgg cagctctgct
     3781 gaccccaccc tctcccggac tgcccttctg tcccagaggg gtcaccctga cccggcccac
     3841 cttgccactg ggctttggac tccagccctg acagggccca gccacactgg ctctgcccct
     3901 cgaaggggct atgagcaagg taggagggag ctggtctcct ttcttcgggc cccacccagg
     3961 ccctgagcac cccccacccc tgtgagggcc ccaggcctta agtccctggc ggggtcatgg
     4021 gtttgcgact tgagcagagc ggaggaacag ggcactggaa ggccgacgag ctcagcatgc
     4081 gactcggtga cggaccaggc tcggcagggc cggtgtactt tttgtggttg tcattggtgt
     4141 gttgttgcac attccaggac gtcagtattt taacaggttc taagtgcctt tctatcgtag
     4201 cttatgtttt cctcctcttg gctccattgc tgttagcata gagttttaaa aaaagagata
     4261 agctaatgac tataacaata tattcctcca tgggagagga agtttataaa gaaacaataa
     4321 aagtgagttg caaagatgag tcgacctgca ggcatgcaag ctgatccggc tgctaacaaa
     4381 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
     4441 ggggcggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
     4501 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     4561 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     4621 ggttcagggg gagatgtggg aggttttttt aagcaagtaa aacctctaca aatgtggtaa
     4681 aatcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac
     4741 gcatctgtgc ggtatttcac accgcatacg cggatctgcg cagcaccatg gcctgaaata
     4801 acctctgaaa gaggaacttg gttaggtacc ttctgaggcg gaaagaacca gctgtggaat
     4861 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4921 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     4981 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     5041 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     5101 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     5161 ggcttttttg gaggcctagg cttttgcaaa aagcttgatt cttctgacac aacagtctcg
     5221 aaccaaaggc tggagccacc atggcttcca aggtgtacga ccccgagcaa cgcaaacgca
     5281 tgatcactgg gcctcagtgg tgggctcgct gcaagcaaat gaacgtgctg gactccttca
     5341 tcaactacta tgattccgag aagcacgccg agaacgccgt gatttttctg catggtaacg
     5401 ctgcctccag ctacctgtgg aggcacgtcg tgcctcacat cgagcccgtg gctagatgca
     5461 tcatccctga tctgatcgga atgggtaagt ccggcaagag cgggaatggc tcatatcgcc
     5521 tcctggatca ctacaagtac ctcaccgctt ggttcgagct gctgaacctt ccaaagaaaa
     5581 tcatctttgt gggccacgac tggggggctt gtctggcctt tcactactcc tacgagcacc
     5641 aagacaagat caaggccatc gtccatgctg agagtgtcgt ggacgtgatc gagtcctggg
     5701 acgagtggcc tgacatcgag gaggatatcg ccctgatcaa gagcgaagag ggcgagaaaa
     5761 tggtgcttga gaataacttc ttcgtcgaga ccatgctccc aagcaagatc atgcggaaac
     5821 tggagcctga ggagttcgct gcctacctgg agccattcaa ggagaagggc gaggttagac
     5881 ggcctaccct ctcctggcct cgcgagatcc ctctcgttaa gggaggcaag cccgacgtcg
     5941 tccagattgt ccgcaactac aacgcctacc ttcgggccag cgacgatctg cctaagatgt
     6001 tcatcgagtc cgaccctggg ttcttttcca acgctattgt cgagggagct aagaagttcc
     6061 ctaacaccga gttcgtgaag gtgaagggcc tccacttcag ccaggaggac gctccagatg
     6121 aaatgggtaa gtacatcaag agcttcgtgg agcgcgtgct gaagaacgag cagaccggtg
     6181 gtgggagcgg aggtggcgga tcaggtggcg gaggctccgg agggattgaa caagatggat
     6241 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     6301 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     6361 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     6421 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     6481 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     6541 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     6601 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     6661 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     6721 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     6781 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     6841 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     6901 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     6961 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     7021 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gatggccgca
     7081 ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagcga
     7141 taaggatcct ctttgcgctt gcgttttccc ttgtccagat agcccagtag ctgacattca
     7201 tccggggtca gcaccgtttc tgcggactgg ctttctacgt aatggtttct tagacgtcag
     7261 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     7321 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     7381 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     7441 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     7501 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     7561 ttcgccccga agaacgtttt ccaatgatga gcactttcaa agttctgcta tgtggcgcgg
     7621 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     7681 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     7741 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     7801 caactatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     7861 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     7921 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     7981 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     8041 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     8101 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     8161 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     8221 taggtgcctc actgattaag cattggtaat tcgaaatgac cgaccaagcg acgcccaacc
     8281 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8341 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8401 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8461 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8521 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8581 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8641 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8701 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8761 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8821 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     8881 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8941 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ttcaagaaga
     9001 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9061 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttatagt ccggaaatac
     9121 aggaacgcac gctggatggc ccttcgctgg gatggtgaaa ccatgaaaaa tggcagcttc
     9181 agtggattaa gtgggggtaa tgtggcctgt accctctggt tgcataggta ttcatacggt
     9241 taaaatttat caggcgcgat tgcggcagtt tttcgggtgg tttgttgcca tttttacctg
     9301 tctgctgccg tgatcgcgct gaacgcgttt tagcggtgcg tacaattaag ggattatggt
     9361 aaatccactt actgtctgcc ctcgtagcca tcgagataaa ccg
//
