LOCUS       sequence-23133-e         632 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Korinek V, Barker N, Morin PJ, van Wichen D, de Weger R, Kinzler KW,
            Vogelstein B, Clevers H
  TITLE     Constitutive transcriptional activation by a beta-catenin-Tcf 
            complex in APC-/- colon carcinoma.
  JOURNAL   Science. 1997 Mar 21. 275(5307):1784-7.
  PUBMED    9065401
REFERENCE   2  (bases 1 to 632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1997 Mar 21. 275(5307):1784-7."
COMMENT     Sequence Label: pHR TCF4
FEATURES             Location/Qualifiers
     source          1..632
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     58..77
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        58..76
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           91..133
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     regulatory      155..164
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             164..193
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gctttagcag agctctctgg ctactagaga acccactgct tactggctta tcgaaattaa
       61 tacgactcac tatagggaga cccaagcttg cttgttcttt ttgcagaagc tcagaataaa
      121 cgctcaactt tggcagatct gatctagaac tagtgccgcc atggagcaga agctgatcag
      181 cgaggaggac ctgcagcccg gggatccgag ctcgaattcc accatgccgc agctgaacgg
      241 cggtggaggg gatgacctag gcgccaacga cgaactgatt tccttcaaag acgagggcga
      301 acgggaggag aagagctccg aaaactcctc ggcagagagg gatttagctg atgtcaaatc
      361 gtctctagtc aatgaatcag aaacgaatca aaacagctcc tccgattccg aggcggaaag
      421 acggcctccg cctcgctccg aaagtttccg agacaaatcc cgggaaagtt tggaagaagc
      481 ggccaagagg caagatggag ggctctttaa ggggccaccg tatcccggct accccttcat
      541 catgatcccc gacctgacga gcccctacct ccccaacgga tcgctctcgc ccaccgcccg
      601 aacctatctc cagatgaaat ggccactgct tg
//
