LOCUS       sequence-231344-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration vector that expresses gfp in S. mutans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Shields RC, Kaspar JR, Lee K, Underhill SAM, Burne RA
  TITLE     Fluorescence tools adapted for real-time monitoring of the behaviors
            of Streptococcus species.
  JOURNAL   Appl Environ Microbiol. 2019 May 17. pii: AEM.00620-19. doi: 
            10.1128/AEM.00620-19.
  PUBMED    31101614
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2019 May 17. pii: AEM.00620-19. doi: 
            10.1128/AEM.00620-19."
COMMENT     Sequence Label: pPM_Pveg-sfgfp
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(109..111,112..825)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 gagctctaga tcgaattcct tattaacgtt gatataattt aaattttatt tgacaaaaat
       61 gggctcgtgt tgtacaataa atgtgattaa ctaataagga ggacaaacat gtcaaaagga
      121 gaagagctgt tcacaggtgt tgtgccgatt ctcgttgagc ttgacggaga tgtaaacgga
      181 cacaaattct ctgttcgcgg tgaaggtgaa ggagatgcaa caaacggcaa gctgacattg
      241 aagtttattt gcacaactgg aaagctgccg gttccttggc cgacacttgt aacgacgctg
      301 acttacggcg ttcaatgctt ctctcgttat ccagaccaca tgaaacgcca tgatttcttc
      361 aaatctgcaa tgcctgaagg ctacgttcaa gagcgtacga tcagcttcaa agatgacgga
      421 acgtacaaaa caagagcaga agtgaagttt gaaggtgaca cacttgtgaa ccgcattgaa
      481 ttgaaaggca ttgatttcaa agaagatgga aacatccttg gacacaaact tgaatacaac
      541 ttcaacagcc acaacgtata catcactgct gacaaacaaa aaaacggcat caaagcaaac
      601 ttcaaaatcc gtcataacgt agaggacggt tctgttcagc ttgctgatca ttatcagcaa
      661 aatacaccga tcggtgacgg cccggttctt cttcctgata accattattt atcaactcaa
      721 agcgtattat caaaagaccc aaatgaaaag cgtgaccaca tggtgctgct tgaatttgtg
      781 acagctgctg gtatcactca cggcatggat gagctttata agtaagcatg c
//
