LOCUS       sequence-231348-       11002 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual luciferase assay for miRNA-targeted UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11002)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 11002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: pmiRGLO-PPM1F-201
FEATURES             Location/Qualifiers
     source          1..11002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     388..407
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      496..505
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             502..2154
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    5974..6095
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5985..6004)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6039..6058
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        6340..6697
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6340..6531
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6363..6383)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6548..6683
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      6742..6751
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6748..7680
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             7732..8523
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7783..7802)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8393..8412
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8587..8635
                     /note="synthetic polyadenylation signal"
     promoter        8791..8895
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8896..8964,8965..9756)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9114..9133)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(9914..10502)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9994..10013)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    10618..10901
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     promoter        join(10958..11002,1..455)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(join(10993..11002,1..14))
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
       61 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
      121 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
      181 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
      241 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
      301 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
      361 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
      421 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttggcaatcc
      481 ggtactgttg gtaaagccac catggaagat gccaaaaaca ttaagaaggg cccagcgcca
      541 ttctacccac tcgaagacgg gaccgccggc gagcagctgc acaaagccat gaagcgctac
      601 gccctggtgc ccggcaccat cgcctttacc gacgcacata tcgaggtgga cattacctac
      661 gccgagtact tcgagatgag cgttcggctg gcagaagcta tgaagcgcta tgggctgaat
      721 acaaaccatc ggatcgtggt gtgcagcgag aatagcttgc agttcttcat gcccgtgttg
      781 ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg acatctacaa cgagcgcgag
      841 ctgctgaaca gcatgggcat cagccagccc accgtcgtat tcgtgagcaa gaaagggctg
      901 caaaagatcc tcaacgtgca aaagaagcta ccgatcatac aaaagatcat catcatggat
      961 agcaagaccg actaccaggg cttccaaagc atgtacacct tcgtgacttc ccatttgcca
     1021 cccggcttca acgagtacga cttcgtgccc gagagcttcg accgggacaa aaccatcgcc
     1081 ctgatcatga acagtagtgg cagtaccgga ttgcccaagg gcgtagccct accgcaccgc
     1141 accgcttgtg tccgattcag tcatgcccgc gaccccatct tcggcaacca gatcatcccc
     1201 gacaccgcta tcctcagcgt ggtgccattt caccacggct tcggcatgtt caccacgctg
     1261 ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga ggagctattc
     1321 ttgcgcagct tgcaagacta taagattcaa tctgccctgc tggtgcccac actatttagc
     1381 ttcttcgcta agagcactct catcgacaag tacgacctaa gcaacttgca cgagatcgcc
     1441 agcggcgggg cgccgctcag caaggaggta ggtgaggccg tggccaaacg cttccaccta
     1501 ccaggcatcc gccagggcta cggcctgaca gaaacaacca gcgccattct gatcaccccc
     1561 gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga ggctaaggtg
     1621 gtggacttgg acaccggtaa gacactgggt gtgaaccagc gcggcgagct gtgcgtccgt
     1681 ggccccatga tcatgagcgg ctacgttaac aaccccgagg ctacaaacgc tctcatcgac
     1741 aaggacggct ggctgcacag cggcgacatc gcctactggg acgaggacga gcacttcttc
     1801 atcgtggacc ggctgaagag cctgatcaaa tacaagggct accaggtagc cccagccgaa
     1861 ctggagagca tcctgctgca acaccccaac atcttcgacg ccggggtcgc cggcctgccc
     1921 gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg taaaaccatg
     1981 accgagaagg agatcgtgga ctatgtggcc agccaggtta caaccgccaa gaagctgcgc
     2041 ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt ggacgcccgc
     2101 aagatccgcg agattctcat taaggccaag aagggcggca agatcgccgt gtaattctag
     2161 ttgtttaaac gaggtggttt ccaggcccct gccctcccct tcctcccatc cttgtccttc
     2221 tctccctcag aagcctcagg acccaacagg tggcaggcag tggacagggt gcccgcccca
     2281 cagtgctttc cccagcaccc cagagccagt cgggacaccc cccgcagccc gtcctggtgg
     2341 ctgtggaact gcactgggtg gcgggcagat ggtggaaggc agcttaggag acctcaccaa
     2401 agagaagatg gaccggctct tgctcccagc tcctattagg cccggggtgg gaccagaggt
     2461 cataggtgcc caacggcagc caaaccaaag acactggtgt gcatggggca gcatggttgt
     2521 gcacgtggga ccctggggcg gacccaggag ccaaactctt gaagcacccc ctgggtcagg
     2581 cccagcagcg gagtggccag ccccagtttc ccattgctcc tctctgcggc cagggccagg
     2641 tgggttcata tttacagata tgcccagcca gtcctggtcg gccacaccag tgtcccaaag
     2701 aggagagcgc agcagagcca ggggtctgtt ctgtagcagc cacccccctg cccccactcc
     2761 agggcagcca tgatgtgctt gggcccacca gggccttccg ggctgctctc ttccctgagc
     2821 ccggaaccgg cgacgcacat gtgtcttttg ttggtgtgtt tgtttttttc cagggaggtc
     2881 taattccgaa gcagtattcc aggttttctc tttgttttat cagtgccaag atgacctgtt
     2941 gtgtcatata atttaagcag agcttagcat ttattttatt ctttagaaaa cttaagtatt
     3001 tactttttta aagctatttt tcaaggaacc tttttttgca gtattattga atttattttc
     3061 taaatcagga ttgaaacagg aacttttcca ggtggtgtta ataagccatt caagtgcctt
     3121 acacagcttt gaagaaacta ggactgcagt gggctcggat aggcccattg aggtttttag
     3181 aaaagcagga tttgttttgt tagggaggca tgattttggt gagatctttc tggaagagtt
     3241 ttccgcctct ttgtgatgct gaacaccccc aaggttctcc cctccccccg ctgcccaggt
     3301 gactggcagg agctgcgact gccacgtagt ggtgcctggg cccgacagcg gggctctggg
     3361 catcccgggt gaccttggcc catctgcctg cattcccacc cccttgggcc tggctggatc
     3421 ccaggcagag ggaccttgct gctgtgtgat tggaacattc ccaaatatct tgtgaatttg
     3481 taatcaaatt ggtctcattg ggaaagactc ttaattaaga ggctcaggca agcacagagg
     3541 cagcccgtgg gtctctgtct cagtctggag gcagcaggga tgctgctggg agtccatggc
     3601 acaggccaca gcccctcacc ttgccgcggt ggctggcagc acgcctgcct tgctctgccc
     3661 catgccctga acaggcatga gagctccacg tcccctagtg caccctgaga gggggctcac
     3721 aagtgaccga tcctgggtgc ctcagggagc tcactgaggg cgtgcaaagt tgaaagtggc
     3781 aaggctgggg gagggtgtcg ggtagaggga agagggcagg gggctagggg aggactcaga
     3841 ggccatctgc agggccaagc cacaggaagg gctgagctgg aggtgggcag ggctgctcca
     3901 ggcaggtcag agcagtgcag ggggaggaga ggagaaaggg aggaagctgg gctgtgtggt
     3961 ccccatgaag gcattcagag tccacctgca gacagcgaga gccccaggaa ggtttgcaca
     4021 gctgtgcccc aagcaccttg gcctcctctc agctcgccga ggaggcacgc tagagccgcc
     4081 ttcccggtgg gagccctctg tcccacaggg agcggggagc cagctttgct ggggccctac
     4141 ctgcatgccc agccttaccc ctcattctca cagcacagat gaggttgaga ccatgcagtc
     4201 aatgcattgc ttaaggtctc ttatttacaa aaaaaaacct taaacatagt cgctgtcatt
     4261 cagacattca gagaatggtt ggccacaaac aatgaccaag tattgcttgg cttaacttga
     4321 aggcctgctg tctccttctg ggggtcaggg acgcagctcc accctcacca ctagcccacc
     4381 ctgcccgtgg gcataacctt gacgaagaga gagaatgatt ggcatctgct tttctctttt
     4441 ctttgctaat aattctgttc ctggctgccg agagtgaagt ttcaccatgt ggaggtttgg
     4501 ctcctatcac ctggtggtct gattcatacc ctagcctgag gctccactgg aagatctcgc
     4561 agcctcagtg tatgggaaac cctttcccca ggcttgtccc agcactgccg ctccccaccc
     4621 ctgagccagg accccagagg atggccatgc cccgtgcctg gcagaggtct ggtgccagca
     4681 ctgggagctg ctccgccctt gccttggggc cgagggagcc ctcgtccacc cctgcacagc
     4741 agctgggcac agaggagcgc tcttccatct tgaccaggac tgcaccaaga agcaccaggt
     4801 gtcttcagcc tccaacctcc ggggcgacct tctcttccag ccacagtccc atgagggccc
     4861 ctagccaggg acactggtct gtaaattgta atcctttctc cagcccagct ctccacttgt
     4921 tccttgtgtg agctgagcag gcagtgcacc tctgagtgtc ccttttgtaa ggcccagggg
     4981 ttgcactgag tctgcagagg ccgcgacctc ctagaacgct gtgggtgcag gtgagccggc
     5041 gtgtcctggg gagatgctgc cagcacacag gggccctcct gctgccagca ggttggggtg
     5101 gttaagtctt attagtgtct attcttaaaa ttaagtgggc tggagaagaa tggagctcca
     5161 catgccagca ccgtatatgg aatacaaaag ctggggaagc agggcctgcc ttacaggtgt
     5221 ggctgactct gagcccaggc ctgcaggggt ggagggcagt ccctcagaat cccagaggca
     5281 gtcccagcct cagaacccag gataggaaat gggtgtgttt agtggggaaa gggacggggt
     5341 gcagacggca gggccagtat ggggccccct ccctctcctc tcctctccta tggtgagccc
     5401 agcgtgggca ccgggccgtc tcagccgtgt tcccagggct gggaggacag ctctggccct
     5461 tcttaggcct agcctcgtcc caagctaaat gtaagccagt tgggctgtgt taaaggaagc
     5521 agtgtttttg gttcgattct gcctctgtag ctcaaggggg gcagccccca gagtcctgtg
     5581 cattctgcca aggctccata gctttgccaa atgcacagag ctctgccatt ccggtgcagt
     5641 gcaggccttg cgaagggttt atctgcgttc gtctcggtgg gcttctcctg catgggagtt
     5701 gtgttcctgt gcaaggggga gctttgctcc aggacaggat gactgtcttc cctattctta
     5761 gggacaagtc ccaagatgcc agaaaggcag tctcccaagg acccaccatg cagaagtgtc
     5821 aataaaccac aagttctgaa ctctgagtcg acctgcaggc atgcaagctg atccggctgc
     5881 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
     5941 accccttggg gcggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa
     6001 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     6061 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     6121 tgtttcaggt tcagggggag atgtgggagg tttttttaag caagtaaaac ctctacaaat
     6181 gtggtaaaat cgaattttaa caaaatatta acgcttacaa tttcctgatg cggtattttc
     6241 tccttacgca tctgtgcggt atttcacacc gcatacgcgg atctgcgcag caccatggcc
     6301 tgaaataacc tctgaaagag gaacttggtt aggtaccttc tgaggcggaa agaaccagct
     6361 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6421 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     6481 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     6541 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6601 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     6661 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttgattctt ctgacacaac
     6721 agtctcgaac caaaggctgg agccaccatg gcttccaagg tgtacgaccc cgagcaacgc
     6781 aaacgcatga tcactgggcc tcagtggtgg gctcgctgca agcaaatgaa cgtgctggac
     6841 tccttcatca actactatga ttccgagaag cacgccgaga acgccgtgat ttttctgcat
     6901 ggtaacgctg cctccagcta cctgtggagg cacgtcgtgc ctcacatcga gcccgtggct
     6961 agatgcatca tccctgatct gatcggaatg ggtaagtccg gcaagagcgg gaatggctca
     7021 tatcgcctcc tggatcacta caagtacctc accgcttggt tcgagctgct gaaccttcca
     7081 aagaaaatca tctttgtggg ccacgactgg ggggcttgtc tggcctttca ctactcctac
     7141 gagcaccaag acaagatcaa ggccatcgtc catgctgaga gtgtcgtgga cgtgatcgag
     7201 tcctgggacg agtggcctga catcgaggag gatatcgccc tgatcaagag cgaagagggc
     7261 gagaaaatgg tgcttgagaa taacttcttc gtcgagacca tgctcccaag caagatcatg
     7321 cggaaactgg agcctgagga gttcgctgcc tacctggagc cattcaagga gaagggcgag
     7381 gttagacggc ctaccctctc ctggcctcgc gagatccctc tcgttaaggg aggcaagccc
     7441 gacgtcgtcc agattgtccg caactacaac gcctaccttc gggccagcga cgatctgcct
     7501 aagatgttca tcgagtccga ccctgggttc ttttccaacg ctattgtcga gggagctaag
     7561 aagttcccta acaccgagtt cgtgaaggtg aagggcctcc acttcagcca ggaggacgct
     7621 ccagatgaaa tgggtaagta catcaagagc ttcgtggagc gcgtgctgaa gaacgagcag
     7681 accggtggtg ggagcggagg tggcggatca ggtggcggag gctccggagg gattgaacaa
     7741 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     7801 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     7861 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     7921 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     7981 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     8041 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     8101 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     8161 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     8221 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     8281 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     8341 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     8401 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     8461 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     8521 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgat
     8581 ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg
     8641 atagcgataa ggatcctctt tgcgcttgcg ttttcccttg tccagatagc ccagtagctg
     8701 acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacgtaat ggtttcttag
     8761 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     8821 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     8881 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     8941 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     9001 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     9061 gagagttttc gccccgaaga acgttttcca atgatgagca ctttcaaagt tctgctatgt
     9121 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     9181 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     9241 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     9301 cttctgacaa ctatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     9361 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     9421 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     9481 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     9541 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     9601 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     9661 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     9721 gctgagatag gtgcctcact gattaagcat tggtaattcg aaatgaccga ccaagcgacg
     9781 cccaaccggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     9841 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     9901 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     9961 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    10021 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    10081 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    10141 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    10201 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    10261 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    10321 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
    10381 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    10441 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatttc
    10501 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    10561 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttatagtccg
    10621 gaaatacagg aacgcacgct ggatggccct tcgctgggat ggtgaaacca tgaaaaatgg
    10681 cagcttcagt ggattaagtg ggggtaatgt ggcctgtacc ctctggttgc ataggtattc
    10741 atacggttaa aatttatcag gcgcgattgc ggcagttttt cgggtggttt gttgccattt
    10801 ttacctgtct gctgccgtga tcgcgctgaa cgcgttttag cggtgcgtac aattaaggga
    10861 ttatggtaaa tccacttact gtctgccctc gtagccatcg agataaaccg cagtactccg
    10921 gccacgatgc gtccggcgta gaggatcgag atctaccggg taggggaggc gcttttccca
    10981 aggcagtctg gagcatgcgc tt
//
