LOCUS       sequence-231373-        8760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual luciferase assay for miRNA-targeted UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8760)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 8760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: pmiRGLO-LEMD2-201
FEATURES             Location/Qualifiers
     source          1..8760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     276..295
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      384..393
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             390..2042
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    3620..3741
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3631..3650)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3685..3704
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        3986..4343
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3986..4177
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4009..4029)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4194..4329
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      4388..4397
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4394..5326
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             5378..6169
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5429..5448)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6039..6058
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6233..6281
                     /note="synthetic polyadenylation signal"
     promoter        6437..6541
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6542..6610,6611..7402)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6760..6779)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(7560..8148)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7640..7659)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    8264..8547
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     promoter        join(8604..8760,1..343)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8639..8662)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
       61 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
      121 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
      181 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
      241 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
      301 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt
      361 ggcaatccgg tactgttggt aaagccacca tggaagatgc caaaaacatt aagaagggcc
      421 cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac aaagccatga
      481 agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc gaggtggaca
      541 ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg aagcgctatg
      601 ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag ttcttcatgc
      661 ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac atctacaacg
      721 agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc gtgagcaaga
      781 aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa aagatcatca
      841 tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc gtgacttccc
      901 atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac cgggacaaaa
      961 ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc gtagccctac
     1021 cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc ggcaaccaga
     1081 tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc ggcatgttca
     1141 ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc ttcgaggagg
     1201 agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg gtgcccacac
     1261 tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc aacttgcacg
     1321 agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg gccaaacgct
     1381 tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc gccattctga
     1441 tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc ttcttcgagg
     1501 ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc ggcgagctgt
     1561 gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct acaaacgctc
     1621 tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac gaggacgagc
     1681 acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac caggtagccc
     1741 cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc ggggtcgccg
     1801 gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg gaacacggta
     1861 aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca accgccaaga
     1921 agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc ggcaagttgg
     1981 acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag atcgccgtgt
     2041 aattctagtt gtttaaacga gctcggcccc gggcggggac ttgttcccgg tcagcctgtc
     2101 ccaggacagc ccactccaga ggcacaggag ggtcaccagg cctgcggtgc tgaattcaca
     2161 cttgccttga ctttcagtct cgtcctgaca cgattccaaa ggtctgccat actcttcaga
     2221 ggagttcggc tcgcaggaaa atgtgggctt cctttaaagg gagggggaga agccagggtg
     2281 atagcccaga ctctgccctg ttgtgctggc agaaacgggg agaagggttg agttttcatg
     2341 aatagaaaac aagagcagca gctgtcaggc ccagctgaca agcgtggggc tggtgggctg
     2401 gggcatggcc acgccaggga gtctgtcacc tgggaggtgg ggcagggatg gtgtttcggg
     2461 tggcgggatg gggagcagct tggtccatgt gccttcaggg ggttgttact tgagagctcc
     2521 agggtgaagg gagaaagcct gtgctctggc tggctgggcc tctatcctga agagggaagg
     2581 tgctagaaat tagaacaagt gactgtaggg gggaccgtct agaaacacca ggcccagaca
     2641 cacggtccct caggtcagga ggagtgagca gccggcacag cctcctggga ggatcccgaa
     2701 gtagggactg gggctgagag cagagaggag ggtcgggcag ccccagggcg ggcgggcccg
     2761 cctcagtgtt gcttctcaca gaagctgcga ctctagacca ggcctgcagc cagaacgccg
     2821 attccgggag cttgggagcc ctgcgtcagg gcccagagcc tcgcacttgc tgtgtgcgaa
     2881 gatcgccttg ctttactgtg accacagccc cgacgcgggg ctgtttaaca agaacttcag
     2941 cgcagccggc gtttctgtta ccccggccgc ggctcttggc ggcgggagag gcgcagtggc
     3001 tgcaggctgc cccctgccgg ccacaaggga gcagcgtccg cgccgccgcc caccggccct
     3061 cccgccagct cctcagcctg ctccctggga ggcaggctcc agaagcaccc cgacgggggg
     3121 ccttattaag tgcgagaagc ctctccagcg agtcttcccc gtgtgtcctc atctgcggca
     3181 ggcaggagag ggggagcaaa caggaaattc cacagctgcc tccagaaaca ttaaccaccc
     3241 ctctctagtc ttactggtat tttttgtttc cttaataaag ctccagtctc cctcatttga
     3301 gcaatactct catttcctct gatgtctgga tgatcggcag ggcaggaatc tgagcgctgg
     3361 ccccgtggtg aggccatgtt ctcataatca ggctccagcc agaaaaataa gaaacaccgc
     3421 aacaggcttc agtatcaggg ctgaaactgc tggatgaata aactatttat taaaaacgga
     3481 tgtttcttgc cagtcgacct gcaggcatgc aagctgatcc ggctgctaac aaagcccgaa
     3541 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcgg
     3601 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     3661 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     3721 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     3781 ggggagatgt gggaggtttt tttaagcaag taaaacctct acaaatgtgg taaaatcgaa
     3841 ttttaacaaa atattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg
     3901 tgcggtattt cacaccgcat acgcggatct gcgcagcacc atggcctgaa ataacctctg
     3961 aaagaggaac ttggttaggt accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc
     4021 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4081 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4141 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     4201 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4261 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     4321 ttggaggcct aggcttttgc aaaaagcttg attcttctga cacaacagtc tcgaaccaaa
     4381 ggctggagcc accatggctt ccaaggtgta cgaccccgag caacgcaaac gcatgatcac
     4441 tgggcctcag tggtgggctc gctgcaagca aatgaacgtg ctggactcct tcatcaacta
     4501 ctatgattcc gagaagcacg ccgagaacgc cgtgattttt ctgcatggta acgctgcctc
     4561 cagctacctg tggaggcacg tcgtgcctca catcgagccc gtggctagat gcatcatccc
     4621 tgatctgatc ggaatgggta agtccggcaa gagcgggaat ggctcatatc gcctcctgga
     4681 tcactacaag tacctcaccg cttggttcga gctgctgaac cttccaaaga aaatcatctt
     4741 tgtgggccac gactgggggg cttgtctggc ctttcactac tcctacgagc accaagacaa
     4801 gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg atcgagtcct gggacgagtg
     4861 gcctgacatc gaggaggata tcgccctgat caagagcgaa gagggcgaga aaatggtgct
     4921 tgagaataac ttcttcgtcg agaccatgct cccaagcaag atcatgcgga aactggagcc
     4981 tgaggagttc gctgcctacc tggagccatt caaggagaag ggcgaggtta gacggcctac
     5041 cctctcctgg cctcgcgaga tccctctcgt taagggaggc aagcccgacg tcgtccagat
     5101 tgtccgcaac tacaacgcct accttcgggc cagcgacgat ctgcctaaga tgttcatcga
     5161 gtccgaccct gggttctttt ccaacgctat tgtcgaggga gctaagaagt tccctaacac
     5221 cgagttcgtg aaggtgaagg gcctccactt cagccaggag gacgctccag atgaaatggg
     5281 taagtacatc aagagcttcg tggagcgcgt gctgaagaac gagcagaccg gtggtgggag
     5341 cggaggtggc ggatcaggtg gcggaggctc cggagggatt gaacaagatg gattgcacgc
     5401 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     5461 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     5521 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     5581 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     5641 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     5701 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     5761 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     5821 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     5881 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     5941 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     6001 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     6061 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     6121 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     6181 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgatggcc gcaataaaat
     6241 atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag cgataaggat
     6301 cctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat tcatccgggg
     6361 tcagcaccgt ttctgcggac tggctttcta cgtaatggtt tcttagacgt caggtggcac
     6421 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6481 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     6541 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     6601 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     6661 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     6721 cgaagaacgt tttccaatga tgagcacttt caaagttctg ctatgtggcg cggtattatc
     6781 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     6841 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     6901 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaactat
     6961 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     7021 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     7081 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     7141 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     7201 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     7261 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     7321 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7381 ctcactgatt aagcattggt aattcgaaat gaccgaccaa gcgacgccca accggtatca
     7441 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     7501 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     7561 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     7621 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     7681 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     7741 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     7801 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     7861 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     7921 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     7981 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8041 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8101 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatttcaaga agatcctttg
     8161 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8221 atgagattat caaaaaggat cttcacctag atccttttat agtccggaaa tacaggaacg
     8281 cacgctggat ggcccttcgc tgggatggtg aaaccatgaa aaatggcagc ttcagtggat
     8341 taagtggggg taatgtggcc tgtaccctct ggttgcatag gtattcatac ggttaaaatt
     8401 tatcaggcgc gattgcggca gtttttcggg tggtttgttg ccatttttac ctgtctgctg
     8461 ccgtgatcgc gctgaacgcg ttttagcggt gcgtacaatt aagggattat ggtaaatcca
     8521 cttactgtct gccctcgtag ccatcgagat aaaccgcagt actccggcca cgatgcgtcc
     8581 ggcgtagagg atcgagatct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
     8641 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
     8701 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
//
