LOCUS       sequence-231374-       10467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual luciferase assay for miRNA-targeted UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10467)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 10467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: pmiRGLO-ADGRL1-201
FEATURES             Location/Qualifiers
     source          1..10467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        53..552
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(88..111)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     485..504
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      593..602
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             599..2251
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    5536..5657
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5547..5566)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5601..5620
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5902..6259
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5902..6093
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5925..5945)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6110..6245
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      6304..6313
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6310..7242
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             7294..8085
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7345..7364)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7955..7974
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8149..8197
                     /note="synthetic polyadenylation signal"
     promoter        8353..8457
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8458..8526,8527..9318)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8676..8695)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(9476..10064)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9556..9575)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    10180..10463
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 aaccgcagta ctccggccac gatgcgtccg gcgtagagga tcgagatcta ccgggtaggg
       61 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
      121 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
      181 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
      241 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
      301 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
      361 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
      421 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc
      481 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
      541 ccgggccttt cgacctgcag cccaagcttg gcaatccggt actgttggta aagccaccat
      601 ggaagatgcc aaaaacatta agaagggccc agcgccattc tacccactcg aagacgggac
      661 cgccggcgag cagctgcaca aagccatgaa gcgctacgcc ctggtgcccg gcaccatcgc
      721 ctttaccgac gcacatatcg aggtggacat tacctacgcc gagtacttcg agatgagcgt
      781 tcggctggca gaagctatga agcgctatgg gctgaataca aaccatcgga tcgtggtgtg
      841 cagcgagaat agcttgcagt tcttcatgcc cgtgttgggt gccctgttca tcggtgtggc
      901 tgtggcccca gctaacgaca tctacaacga gcgcgagctg ctgaacagca tgggcatcag
      961 ccagcccacc gtcgtattcg tgagcaagaa agggctgcaa aagatcctca acgtgcaaaa
     1021 gaagctaccg atcatacaaa agatcatcat catggatagc aagaccgact accagggctt
     1081 ccaaagcatg tacaccttcg tgacttccca tttgccaccc ggcttcaacg agtacgactt
     1141 cgtgcccgag agcttcgacc gggacaaaac catcgccctg atcatgaaca gtagtggcag
     1201 taccggattg cccaagggcg tagccctacc gcaccgcacc gcttgtgtcc gattcagtca
     1261 tgcccgcgac cccatcttcg gcaaccagat catccccgac accgctatcc tcagcgtggt
     1321 gccatttcac cacggcttcg gcatgttcac cacgctgggc tacttgatct gcggctttcg
     1381 ggtcgtgctc atgtaccgct tcgaggagga gctattcttg cgcagcttgc aagactataa
     1441 gattcaatct gccctgctgg tgcccacact atttagcttc ttcgctaaga gcactctcat
     1501 cgacaagtac gacctaagca acttgcacga gatcgccagc ggcggggcgc cgctcagcaa
     1561 ggaggtaggt gaggccgtgg ccaaacgctt ccacctacca ggcatccgcc agggctacgg
     1621 cctgacagaa acaaccagcg ccattctgat cacccccgaa ggggacgaca agcctggcgc
     1681 agtaggcaag gtggtgccct tcttcgaggc taaggtggtg gacttggaca ccggtaagac
     1741 actgggtgtg aaccagcgcg gcgagctgtg cgtccgtggc cccatgatca tgagcggcta
     1801 cgttaacaac cccgaggcta caaacgctct catcgacaag gacggctggc tgcacagcgg
     1861 cgacatcgcc tactgggacg aggacgagca cttcttcatc gtggaccggc tgaagagcct
     1921 gatcaaatac aagggctacc aggtagcccc agccgaactg gagagcatcc tgctgcaaca
     1981 ccccaacatc ttcgacgccg gggtcgccgg cctgcccgac gacgatgccg gcgagctgcc
     2041 cgccgcagtc gtcgtgctgg aacacggtaa aaccatgacc gagaaggaga tcgtggacta
     2101 tgtggccagc caggttacaa ccgccaagaa gctgcgcggt ggtgttgtgt tcgtggacga
     2161 ggtgcctaaa ggactgaccg gcaagttgga cgcccgcaag atccgcgaga ttctcattaa
     2221 ggccaagaag ggcggcaaga tcgccgtgta attctagttg tttaaacgag ctcggggcac
     2281 ctcatggacc aggggctggt ggcccaggcc agggagggaa ccctgggcag ggctctggtg
     2341 ggagagggag acagatggag gcagtggctg gtgggccact ctctccaggt gcccctcagc
     2401 catgggccct acagtcccct caggggactc taacctgggg gcctgaggtg ccagggttca
     2461 cagacagggt ttcccaccag ccacacgcac cagctctatt tgggggaagt gtagtgagga
     2521 ggagcccaga ggaccccagg ggagtgagga gggagaactt ggaagggtgc agcccacttc
     2581 cagactctcc cctctcccac ccttctaccc tgtgaaggga aatgagggct ttagtttcct
     2641 gggcagggag gggcagcttc tgaggttgcc aaaggccccc actggatgga acctgttagc
     2701 tgctcctctc cgcagccaga aatgctgccg gctgcaccca gagggagcag tgaggcagga
     2761 cagatggaca ggttcctcct gcgctgtaat tccctgctcc ctggagactg ggaaaaggcc
     2821 gcagggcagg gggactgggc ggtggtggct ggtggtttaa aggttgaact ttctctgaag
     2881 ctcctttccc cttgctcttg gtccctgccc cgcaagcaaa cctgccccct ctgcctccca
     2941 gtgcacccaa tgaccccctc ccttggggcg actcctgatg aagcacaact ccccgcaggg
     3001 cccccagccc acaggggtgg ccatatttgg gcagttccca gtcctgtggg ctcggctatc
     3061 tggggagcag attttgggtc tggatctccc tggggagtgg gtcctgggct tggatctttc
     3121 cctagggggc cctcttactc cttcctctct cctcctcctt ccccattgct gtaaatattt
     3181 caacgaaatg gaaaagaaaa aaaaaagaca aaaaaaaaaa aaaaagaaaa gaaaaaaaat
     3241 ctcatcactt gaagccaccg ggagcctgcg gcccgggcca gcccgggctt tctccggagc
     3301 agagcagcgc tgctggcctg gcagccagga cttctccgtg tacgtgcatc cccagtcccc
     3361 gccggggcgg gcgggccgcc agagcagctt tttacaatcc agagacagaa aacaaaatct
     3421 agatgcaaca gcgaaacaaa gaacccattt ccttcctggc acccgtttct gtcccctcct
     3481 ttcctgctcc gcctgcccgg catcatcagc ccttctgggg cgtgtacctc tcactgcctg
     3541 ccccaggacc agaggcctgt ctcccccttc cctccccacc ggcccggggg agacctcttc
     3601 acacccaaag atgctttgtc aagatggctg cgctgtccct ttgagttcct gcttcttgta
     3661 tattgctctg gggactctcg ccggggaaga tggggctgat ttcccaccta gagaggttga
     3721 tggcagaagg aaaatgggga ggcactgggg ttgtggaggg catgggtatg gctgaggagg
     3781 gtgctgggaa cggcaaggcg gtctggggga tggggagggg caaaggtgag agatcacctt
     3841 ccctatcctc caccttcccg cagggaagac gaagccaggt gccagggctg gggtcgggac
     3901 acccatgttc tgggcggctt gaccccaaat cccagacccc ggaagctcca ggctcgggat
     3961 gtgtcctgag cacctgtccc ggccccctcc tctccagcct gtcaggccag cctccccagc
     4021 ccccttccct caactctagg ggcagcatag ccccaggcca ccctagccca gcccctgcat
     4081 tgccgtttcc ccagaggtgg cccctccacc tctgctctga cctctcccca tctctctgcc
     4141 cctccttttc tcacaagtgc catgagtttt caaacatctt cgggtctcag cctgctgctg
     4201 ccatgaactt tgttgggtta agggggaggg gctctaggaa ggaactgggg ggaaggggac
     4261 aggtaggtgg ctggagggac cctttttgct gctagaaagc ccctcccttc tcccggggag
     4321 ctggggctgg cttgtcccca tcaattaggg gagaaggggt cagaccaaag atggtggttt
     4381 gtcccgtgta agggaacagg tgtggggaag aggtggggtt gggttccaac tctggctttt
     4441 tctggtagaa aggcctaacc cattacacca gaagaccatg ggtgaagtct agatggggag
     4501 gagaagtgag gccagggaca gtcttgccag acgactctga ggagggcaag ggacagttcc
     4561 atggcccttc ccggactgca ctaggctggg agctctgttg tgtggagggg tggtcagagg
     4621 tctgtgaaga gcgggcagga ggcatgagca ggcgaggagc tggcctgtcc cgggaagcag
     4681 cccagcctgt ggctgccccc gacaccagga ggagggccag gagagttcca gggggctgag
     4741 gcacacagag aggccaactt cctccctgcc cagccttcct tgtatctctc cagactcgga
     4801 caatcaaagg gagagagggt gtatcgggtc ctgttccagc cggcatcgcc gggtggcttc
     4861 caggcctcag agctgtgtgg cagggccccc tgctggggct ggacatcact gcagtccagt
     4921 gcaaagccgc ccccagagcc caggtgtccc cccaacctaa ccagacctgg tgccttgacg
     4981 cccccacccc agctgggacg gtacagagaa gggtcttggt ttcctctcct actcccttcc
     5041 ttaggctcct gaactcgttt gctcctaaat cttgttaatt ctttttctct ggattttggt
     5101 ttcttttggc tttcccttgc cttccccttt ctctgtctcc aacactcttt ccccatgtct
     5161 ttctggctgt ctctatgttc ctcttctctt atcctcaact ttctgtccat tcgggcctcc
     5221 tccccacctc ccacgcccca gcccctccct ccttggtctc cttttcgata tgccaaacca
     5281 attttgggtc gagtgcattt aacgagaaca aaacaaaagg ctcataacaa caagaacgtt
     5341 tcagaaaaaa acaaaaagtt ttaaaaaaat tgttgagtca aaaagtcaaa caataaagaa
     5401 attaagaagt cgacctgcag gcatgcaagc tgatccggct gctaacaaag cccgaaagga
     5461 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcggccgc
     5521 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     5581 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     5641 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     5701 agatgtggga ggttttttta agcaagtaaa acctctacaa atgtggtaaa atcgaatttt
     5761 aacaaaatat taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg
     5821 gtatttcaca ccgcatacgc ggatctgcgc agcaccatgg cctgaaataa cctctgaaag
     5881 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     5941 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     6001 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     6061 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     6121 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     6181 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     6241 aggcctaggc ttttgcaaaa agcttgattc ttctgacaca acagtctcga accaaaggct
     6301 ggagccacca tggcttccaa ggtgtacgac cccgagcaac gcaaacgcat gatcactggg
     6361 cctcagtggt gggctcgctg caagcaaatg aacgtgctgg actccttcat caactactat
     6421 gattccgaga agcacgccga gaacgccgtg atttttctgc atggtaacgc tgcctccagc
     6481 tacctgtgga ggcacgtcgt gcctcacatc gagcccgtgg ctagatgcat catccctgat
     6541 ctgatcggaa tgggtaagtc cggcaagagc gggaatggct catatcgcct cctggatcac
     6601 tacaagtacc tcaccgcttg gttcgagctg ctgaaccttc caaagaaaat catctttgtg
     6661 ggccacgact ggggggcttg tctggccttt cactactcct acgagcacca agacaagatc
     6721 aaggccatcg tccatgctga gagtgtcgtg gacgtgatcg agtcctggga cgagtggcct
     6781 gacatcgagg aggatatcgc cctgatcaag agcgaagagg gcgagaaaat ggtgcttgag
     6841 aataacttct tcgtcgagac catgctccca agcaagatca tgcggaaact ggagcctgag
     6901 gagttcgctg cctacctgga gccattcaag gagaagggcg aggttagacg gcctaccctc
     6961 tcctggcctc gcgagatccc tctcgttaag ggaggcaagc ccgacgtcgt ccagattgtc
     7021 cgcaactaca acgcctacct tcgggccagc gacgatctgc ctaagatgtt catcgagtcc
     7081 gaccctgggt tcttttccaa cgctattgtc gagggagcta agaagttccc taacaccgag
     7141 ttcgtgaagg tgaagggcct ccacttcagc caggaggacg ctccagatga aatgggtaag
     7201 tacatcaaga gcttcgtgga gcgcgtgctg aagaacgagc agaccggtgg tgggagcgga
     7261 ggtggcggat caggtggcgg aggctccgga gggattgaac aagatggatt gcacgcaggt
     7321 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     7381 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     7441 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     7501 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     7561 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     7621 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     7681 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     7741 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     7801 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     7861 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     7921 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     7981 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     8041 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     8101 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg atggccgcaa taaaatatct
     8161 ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagcgat aaggatcctc
     8221 tttgcgcttg cgttttccct tgtccagata gcccagtagc tgacattcat ccggggtcag
     8281 caccgtttct gcggactggc tttctacgta atggtttctt agacgtcagg tggcactttt
     8341 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     8401 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     8461 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     8521 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     8581 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     8641 gaacgttttc caatgatgag cactttcaaa gttctgctat gtggcgcggt attatcccgt
     8701 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     8761 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     8821 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aactatcgga
     8881 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     8941 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     9001 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     9061 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     9121 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     9181 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     9241 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     9301 ctgattaagc attggtaatt cgaaatgacc gaccaagcga cgcccaaccg gtatcagctc
     9361 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     9421 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     9481 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     9541 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     9601 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     9661 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     9721 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     9781 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     9841 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     9901 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     9961 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    10021 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatt tcaagaagat cctttgatct
    10081 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    10141 gattatcaaa aaggatcttc acctagatcc ttttatagtc cggaaataca ggaacgcacg
    10201 ctggatggcc cttcgctggg atggtgaaac catgaaaaat ggcagcttca gtggattaag
    10261 tgggggtaat gtggcctgta ccctctggtt gcataggtat tcatacggtt aaaatttatc
    10321 aggcgcgatt gcggcagttt ttcgggtggt ttgttgccat ttttacctgt ctgctgccgt
    10381 gatcgcgctg aacgcgtttt agcggtgcgt acaattaagg gattatggta aatccactta
    10441 ctgtctgccc tcgtagccat cgagata
//
