LOCUS       sequence-231415-       12921 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  plasmid expressing translational fusions of AcrAB and MutS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12921)
  AUTHORS   El Meouche I, Dunlop MJ
  TITLE     Heterogeneity in efflux pump expression predisposes 
            antibiotic-resistant cells to mutation.
  JOURNAL   Science. 2018 Nov 9;362(6415):686-690. doi: 10.1126/science.aar7981.
  PUBMED    30409883
REFERENCE   2  (bases 1 to 12921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2018-11-9- 9"; volume: "362"; issue: 
            "6415"; pages: "686-690"
COMMENT     Sequence Label: PacrAB-acrAB-rfp + PmutS-mutS-yfp
FEATURES             Location/Qualifiers
     source          1..12921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..714
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(31..59)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     654..674
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      747..833
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      879..950
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      966..993
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1524..2474)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     terminator      complement(3236..3330)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3361..4155)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3472..3491)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4082..4101
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     RBS             9634..9645
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(10107..10138)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             10906..10917
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
       61 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      121 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      181 gtcactactt tcggttatgg tctaaaatgc tttgctagat acccagatca tatgaaacgg
      241 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      301 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      361 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      421 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
      481 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      541 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      601 ctgtcctatc aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
      661 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataatag
      721 gcttaattag ctgagtctag aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      781 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgc
      841 cctagaggat ccaaactcga gtaaggatct ccaggcatca aataaaacga aaggctcagt
      901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      961 acactggctc accttcgggt gggcctttct gcgtttatac ctagggtacg ggttttgctg
     1021 cccgcaaacg ggctgttctg gtgttgctag tttgttatca gaatcgcaga tccggcttca
     1081 gccggtttgc cggctgaaag cgctatttct tccagaattg ccatgatttt ttccccacgg
     1141 gaggcgtcac tggctcccgt gttgtcggca gctttgattc gataagcagc atcgcctgtt
     1201 tcaggctgtc tatgtgtgac tgttgagctg taacaagttg tctcaggtgt tcaatttcat
     1261 gttctagttg ctttgtttta ctggtttcac ctgttctatt aggtgttaca tgctgttcat
     1321 ctgttacatt gtcgatctgt tcatggtgaa cagctttgaa tgcaccaaaa actcgtaaaa
     1381 gctctgatgt atctatcttt tttacaccgt tttcatctgt gcatatggac agttttccct
     1441 ttgatatgta acggtgaaca gttgttctac ttttgtttgt tagtcttgat gcttcactga
     1501 tagatacaag agccataaga acctcagatc cttccgtatt tagccagtat gttctctagt
     1561 gtggttcgtt gtttttgcgt gagccatgag aacgaaccat tgagatcata cttactttgc
     1621 atgtcactca aaaattttgc ctcaaaactg gtgagctgaa tttttgcagt taaagcatcg
     1681 tgtagtgttt ttcttagtcc gttatgtagg taggaatctg atgtaatggt tgttggtatt
     1741 ttgtcaccat tcatttttat ctggttgttc tcaagttcgg ttacgagatc catttgtcta
     1801 tctagttcaa cttggaaaat caacgtatca gtcgggcggc ctcgcttatc aaccaccaat
     1861 ttcatattgc tgtaagtgtt taaatcttta cttattggtt tcaaaaccca ttggttaagc
     1921 cttttaaact catggtagtt attttcaagc attaacatga acttaaattc atcaaggcta
     1981 atctctatat ttgccttgtg agttttcttt tgtgttagtt cttttaataa ccactcataa
     2041 atcctcatag agtatttgtt ttcaaaagac ttaacatgtt ccagattata ttttatgaat
     2101 ttttttaact ggaaaagata aggcaatatc tcttcactaa aaactaattc taatttttcg
     2161 cttgagaact tggcatagtt tgtccactgg aaaatctcaa agcctttaac caaaggattc
     2221 ctgatttcca cagttctcgt catcagctct ctggttgctt tagctaatac accataagca
     2281 ttttccctac tgatgttcat catctgagcg tattggttat aagtgaacga taccgtccgt
     2341 tctttccttg tagggttttc aatcgtgggg ttgagtagtg ccacacagca taaaattagc
     2401 ttggtttcat gctccgttaa gtcatagcga ctaatcgcta gttcatttgc tttgaaaaca
     2461 actaattcag acatacatct caattggtct aggtgatttt aatcactata ccaattgaga
     2521 tgggctagtc aatgataatt actagtcctt ttcccgggtg atctgggtat ctgtaaattc
     2581 tgctagacct ttgctggaaa acttgtaaat tctgctagac cctctgtaaa ttccgctaga
     2641 cctttgtgtg ttttttttgt ttatattcaa gtggttataa tttatagaat aaagaaagaa
     2701 taaaaaaaga taaaaagaat agatcccagc cctgtgtata actcactact ttagtcagtt
     2761 ccgcagtatt acaaaaggat gtcgcaaacg ctgtttgctc ctctacaaaa cagaccttaa
     2821 aaccctaaag gcttaagtag caccctcgca agctcgggca aatcgctgaa tattcctttt
     2881 gtctccgacc atcaggcacc tgagtcgctg tctttttcgt gacattcagt tcgctgcgct
     2941 cacggctctg gcagtgaatg ggggtaaatg gcactacagg cgccttttat ggattcatgc
     3001 aaggaaacta cccataatac aagaaaagcc cgtcacgggc ttctcagggc gttttatggc
     3061 gggtctgcta tgtggtgcta tctgactttt tgctgttcag cagttcctgc cctctgattt
     3121 tccagtctga ccacttcgga ttatcccgtg acaggtcatt cagactggct aatgcaccca
     3181 gtaaggcagc ggtatcatca acaggcttac ccgtcttact gtccctagtg cttggattct
     3241 caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
     3301 aggtcattac tggatctatc aacaggagtc caagcgagct ctcgaacccc agagtcccgc
     3361 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
     3421 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg
     3481 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
     3541 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac
     3601 gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc
     3661 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
     3721 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
     3781 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
     3841 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc
     3901 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg
     3961 cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac
     4021 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg
     4081 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc
     4141 atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatcagatc atgatcccct
     4201 gcgccatcag atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac
     4261 cttaccagag ggcgccccag ctggcaattc cgacgtcggt gcctaatgag tgagctaact
     4321 tacattaatt gcgttgcgcg ctgatgtggt ggagatcgaa tttcaaaggt tagttttttc
     4381 atgacttccc tctcccccaa ataaaaaggc ctgctggcga ttaccagcca gcaggcctag
     4441 tattaaccgt gtccttctcg atttgaataa ataaaaacag ccgttgccag aaagaggcac
     4501 ggctgttttt attttctatt atttgtacag ctcatccatg ccaccggtag aatgacgacc
     4561 ctccgcgcgc tcatattgct ctacgatcgt ataatcttca ttatgagagg tgatgtccag
     4621 tttaatattc acattgtacg cgccaggcag ctgcacaggt ttcttggctt tgtacgtggt
     4681 tttcacttca gcgtcataat ggccgccatc tttcagtttc aggcgctgtt taatttcgcc
     4741 tttcagcgca ccatcttccg gatacatgcg ttcgctagac gcctcccaac ccatcgtctt
     4801 tttctgcatc accgggccat cagatggaaa attagtacca cgcagtttaa ctttatagat
     4861 gaactcgcca tcttgcaggg aggagtcctg agtgacggtc acgacaccac catcttcaaa
     4921 attcattacg cgttcccact tgaaaccttc cggaaaagac agcttcagat agtccgggat
     4981 atccgctggg tgtttaacat acgctttaga accgtacata aattgcgggc tcaggatgtc
     5041 ccacgcaaaa ggcagcgggc cgcctttagt cactttcagt ttggcggtct gggtgccttc
     5101 atacggacgg ccctcgcctt cgccttcgat ctcgaactcg tgaccgttaa cagaaccctc
     5161 catgtgaact ttgaagcgca tgaactcttt aatgatagcc atgttatcct cctcgccctt
     5221 ggatcttttg ctagtcattc cctgtccttt gttaccggaa ccatgatgat cgacagtatg
     5281 gctgtgctcg atatcttcat tcttgcggct aaagcggcgg cgaaccacca caaagaatac
     5341 cggaacgaag aagattgcca gtaccgttgc ggtcaccatc ccgcccatta caccggtacc
     5401 tactgcgttc tgcgcgccgg aaccagcacc agtactgata accagcggca taacgccgag
     5461 gataaacgcc agcgaggtca tcaggatcgg acgtaaacgc atccgcaccg catcaagcgt
     5521 cgcttcaatc agacctttac cttctttatc catcaagtct ttggcgaatt cgacgataag
     5581 gatcgcgttc ttcgccgaca acccaatggt tgtgagcagg cctacctgga agtaaacgtc
     5641 attggtcagg ccacggaagg tggcagccag caacgcaccg ataaccccca gcggaacgac
     5701 cagcataacg gagaacggaa tcgaccagct ctcgtacagc gccgccagac acaggaacac
     5761 gacaatcaac gaaatcgcgt acagtgaagg tgcctggttg ccggagagac gttcctgata
     5821 ggacatcccc gtccagtcat agccaacacc ggtaggcagt ttgctcgcca gttgttccat
     5881 cagctccatt gcttcaccgg tacttttacc cggtgccgcc tggcctaaga tttccatgga
     5941 tggcaggccg ttgtaacgtt ccagacgcgg cgaaccgtac tcccaacgag aagaggagaa
     6001 cgccgagaat ggcaccatct gaccatcagc agcacgaaca taccagtcgc cgatatcatc
     6061 cggcagcata cggtatttcg cttctgacat gacataaact ttcttcacac gaccgcggtc
     6121 gataaagtcg ttcacatagc tgccgcccca tgcagcgccc agagtggtgt taatgtcgtt
     6181 gatagaaaca cccagcgcct gcgctttttc ctggtcgata tcaatcttaa actgcggggt
     6241 atcttccaga ccgtttggac gtacgctggt caacatatca gggtgcttcg ctgcttctgc
     6301 aagcaactgg ttacgcgcct gagtcagttt ttcgtgacca aggccagcct ggtcaatcag
     6361 ctcaaagtca aagccggttg cagtacccag ttccacgatt gcgggcaggt taaaggcgaa
     6421 aaccatcgca tctttgattt gcgagaaagc gcgtgttgca cgcatggtaa tcgcttcaac
     6481 tttgttttct tcgcccggac gatcggccca gtccttcaag gaaacgaacg caataccggt
     6541 attctgacca cgtcccgcaa agccgaagcc gttaacggcg aacaccgact caacgttgtt
     6601 cttttctttg gtcagatagt aatgcgttac ctcattgagc actttctgtg tacgttcctg
     6661 cgttgcacct gctggcagct gaaccatggt cataaacacg ccctggtcct catctggcaa
     6721 gaaggagctt ggcagacgca cgaacagata ggccatgccg accacgatga tcagatacag
     6781 caccaggtaa cgccccgtac tgcgcagaat accgcctacg ctgtcggtgt agtggtgcgt
     6841 gctcttctcg aacatgcggt taaaccagcc gaagaagcct tttttacctt ccccgtgatc
     6901 gcctttggca atcggtttca gcatggtggc acaaagagct ggagtcagga tcaacgccac
     6961 cagtaccgac agcgccattg ctgaaacaat ggtaatagag aactgacgat agatagcacc
     7021 agtagaaccg ccaaagaagg ccatcggtac gaataccgcc gacagtacca tcgcgatacc
     7081 gaccagagcg ccctgaatct gccccatcga cttacgggta gcttcttttg gcggcaaacc
     7141 ttcttccgcc ataacacgct caacgttttc taccacaacg atggcgtcat ccaccaacag
     7201 gccgatggcg agcaccatcc cgaacattgt tagcgtgttt atcgagaagc caaaggcggc
     7261 aaggacggca aaggtcccga gcaataccac cggtacggca atggtcggaa tcaacgtcgc
     7321 gcggaagttc tgcaggaaca gatacataac caggaacacg aggatgatcg cttcgaccag
     7381 cgttttaacc acttcgtgaa tagagatttt cacgaacggc gtggtgtcgt atgggtaaac
     7441 aattttcaga cccgacggga agaacggttc catcttcgcc agttcagcac ggattgccgc
     7501 agcggtatcc agcgcgtttg caccggtcgc cagcttgatc cccagaccgg aagccggttg
     7561 gccgttaaac tctgcgatga tgtcgtagtt ctcaccaccc agctcaatct tcgcgacgtc
     7621 acgcagcagc acgcgggaac catcctgatt cactttcagc aggattttgc cgaactcttc
     7681 agtagaggtc agacgcgtct gagcaataat agaggcgtta agctgttggc ctttcaccgg
     7741 cggcgtacca ccgagctgac ccgccgcaac ctgggcgttc tgcgctttga tggcggtaat
     7801 gacatcaacc ggcgttagct ggaatttgtt cagctcattc gggttcatcc agatacgcat
     7861 cgcgtactgt gaaccgaaca actgaacatc acccacgccc gacgtacggc tgatggcatc
     7921 tttcatattc gccgccacgt agtcggagat atcctcctgc gtcatggtgc catcggtgtt
     7981 gataacgccg acaaccatca ggaagctgct ggatgatttc tcaacgctca ccccttgctg
     8041 ctgaacttct tgcggcagca acggcatcgc cagctgcagt ttgttctgca cctgaacctg
     8101 cgcgatatcc gcatcagtac cagactcaaa ggtcagggtg atctgcacgg tacccgtgga
     8161 gtcactgtta gaggacatgt acatcaggtt atcgataccg ttcatattct gttcgataac
     8221 ctgtgtcacc gtgtcctgca ctgttttcgc atcagcgccg gggtaggagg cggagatcgt
     8281 tactgccggc ggtgcaatcg taggatattg cgccaccggc agtttgagga tcgccagccc
     8341 ccctgccaac atgatgataa tggcgatcac ccacgcaaaa atcgggcgat cgataaagaa
     8401 attaggcatg tcttaacggc tcctgtttaa gttaagactt ggactgttca ggctgagcac
     8461 cgcttgcggc ttgctggtta ttatcagcgg taacttcttg tgcttttacc tggacaccag
     8521 gacgcacttt ctgcagccca cttattacta cgcgatcgcc tgctttcaga ccttctgtca
     8581 ccagccactt atcgccaata gcctggcttg caacgatcgg acgggtttcc actttgtcat
     8641 ccgcgccaac taccagtacg gtggcatcgc cacgcggcgt acgggttacg ccctgttgcg
     8701 ggactaaaat agcgtttgga ttaagccctt cttccagacg tgcgcgcacg aacatacccg
     8761 gcagcagagt gtgatccggg ttcgggaaga tagcgcgtag ggtgatagac ccagtggtct
     8821 gatcaacggt aacgtcagag aattccagcg taccgtcctg cgggaactta atgccgtcac
     8881 tggtgatcag tgacactttg gctttgccgt tctcttgttt cagcgtgcca ttcgccagtt
     8941 cctgtttcag gcgcaggaag tcgttgctgg actgggtcac atcaacgtag atcggatcaa
     9001 gttgctgcac ggttgccagc gcagtcgcct gaccgttctg taccaatgcg ccttccgtca
     9061 cgttcgactt accaatgcga ccgctaatcg gagaggtgac tttggtgtaa gccagattga
     9121 tccgcgcagt ttcaacggca gctttcgccg cagttaccgc agcattcgcc tgttgcgcat
     9181 cagccagagc ctgatcgtac tcttgcttac tgatgtactg agtaccgagc agtttctgat
     9241 aacgattcac cgtcaattgc gcgatattgg ctgcagcctg ggctttcgcc agatcacctt
     9301 tcgcactgtc gtatgtcgcc tgataggtcg caggatcaat ctgatagaga gagacacctg
     9361 cttcgatgtc gctaccttct ttgaaattac gcttcaggat aatcccgcta acttgaggac
     9421 gaacttctgc gatccggtag gcactggtgc gacccggaag ctcggttgtg atctgcagag
     9481 gttcagtttt gactgttact acgccaacgg cgggcatctg ctggccacct tgttgggcct
     9541 gtttgtcgtc acatcctgtt agggctaagc tgcctgagag catcagaacg accgccagag
     9601 gcgtaaaccc tctgtttttg ttcatgcggt acctttctcc tctttaaagt taaagatctt
     9661 cgtgctatgg tacatacatt cacaaatgta tgtaaatcta acgcctgtaa attcacgaac
     9721 atatggcacg aaaaaccaaa caagaagcgc aagaaacgcg ccaacacatc ctcgatgtgg
     9781 ctctacgtct tttctcacag cagggggtat catccacctc gctgggcgag attgcaaaag
     9841 cagctggcgt tacgcgcggt gcaatctact ggcgtcgacg atccggcaaa caaaccaccg
     9901 ttggtagcgg tggttttttt gtttggatcg acaatcttcg taagcgtcat caataagcgt
     9961 aaaaaaaccg ggcaatgccc ggttttttaa tgagaaattt tacctgtcgt agccgccacc
    10021 atccggcaaa gaagcataca aggcttttgg cttatagcta cgtagcgcat tgcgtcgcag
    10081 cacaatcccg gcaccgatcg agtaatagtc aaaagcctcc ggtcggaggc ttttgacttt
    10141 ctgcccgggg cgtacttgct tcataagcat cacgcaaaag ctgcaaaaca gcatctttcc
    10201 cggaaccagc atcaagaact cgccgttcgc ttcttcccct gaaatgatta actccggtat
    10261 catgtgcgcc ttatgtgatt acaacgaaaa taaaaaccat cacaccccat ttaatatcag
    10321 ggaaccggac ataaccccat gagtgcaata gaaaatttcg acgcccatac gcccatgatg
    10381 cagcagtatc tcaggctgaa agcccagcat cccgagatcc tgctgtttta ccggatgggt
    10441 gatttttatg aactgtttta tgacgacgca aaacgcgcgt cgcaactgct ggatatttca
    10501 ctgaccaaac gcggtgcttc ggcgggagag ccgatcccga tggcggggat tccctaccat
    10561 gcggtggaaa actatctcgc caaactggtg aatcagggag agtccgttgc catctgcgaa
    10621 caaattggcg atccggcgac cagcaaaggt ccggttgagc gcaaagttgt gcgtatcgtt
    10681 acgccaggca ccatcagcga tgaagccctg ttgcaggagc gtcaggacaa cctgctggcg
    10741 gctatctggc aggacagcaa aggtttcggc tacgcgacgc tggatatcag ttccgggcgt
    10801 tttcgcctga gcgaaccggc tgaccgcgaa acgatggcgg cagaactgca acgcactaat
    10861 cctgcggaac tgctgtatgc agaagatttt gctgaaatgt cgttaattga aggccgtcgc
    10921 ggcctgcgcc gtcgcccgct gtgggagttt gaaatcgaca ccgcgcgcca gcagttgaat
    10981 ctgcaatttg ggacccgcga tctggtcggt tttggcgtcg agaacgcgcc gcgcggactt
    11041 tgtgctgccg gttgtctgtt gcagtatgcg aaagataccc aacgtacgac tctgccgcat
    11101 attcgttcca tcaccatgga acgtgagcag gacagcatca ttatggatgc cgcgacgcgt
    11161 cgtaatctgg aaatcaccca gaacctggcg ggtggtgcgg aaaatacgct ggcttctgtg
    11221 ctcgactgca ccgtcacgcc gatgggcagc cgtatgctga aacgctggct gcatatgcca
    11281 gtgcgcgata cccgcgtgtt gcttgagcgc cagcaaacta ttggcgcatt gcaggatttc
    11341 accgccgggc tacagccggt actgcgtcag gtcggcgacc tggaacgtat tctggcacgt
    11401 ctggctttac gaactgctcg cccacgcgat ctggcccgta tgcgccacgc tttccagcaa
    11461 ctgccggagc tgcgtgcgca gttagaaact gtcgatagtg caccggtaca ggcgctacgt
    11521 gagaagatgg gcgagtttgc cgagctgcgc gatctgctgg agcgagcaat catcgacaca
    11581 ccgccggtgc tggtacgcga cggtggtgtt atcgcatcgg gctataacga agagctggat
    11641 gagtggcgcg cgctggctga cggcgcgacc gattatctgg agcgtctgga agtccgcgag
    11701 cgtgaacgta ccggcctgga cacgctgaaa gttggcttta atgcggtgca cggctactac
    11761 attcaaatca gccgtgggca aagccatctg gcacccatca actacatgcg tcgccagacg
    11821 ctgaaaaacg ccgagcgcta catcattcca gagctaaaag agtacgaaga taaagttctc
    11881 acctcaaaag gcaaagcact ggcactggaa aaacagcttt atgaagagct gttcgacctg
    11941 ctgttgccgc atctggaagc gttgcaacag agcgcgagcg cgctggcgga actcgacgtg
    12001 ctggttaacc tggcggaacg ggcctatacc ctgaactaca cctgcccgac cttcattgat
    12061 aaaccgggca ttcgcattac cgaaggtcgc catccggtag ttgaacaagt actgaatgag
    12121 ccatttatcg ccaacccgct gaatctgtcg ccgcagcgcc gcatgttgat catcaccggt
    12181 ccgaacatgg gcggtaaaag tacctatatg cgccagaccg cactgattgc gctgatggcc
    12241 tacatcggca gctatgtacc ggcacaaaaa gtcgagattg gacctatcga tcgcatcttt
    12301 acccgcgtag gcgcggcaga tgacctggcg tccgggcgct caacctttat ggtggagatg
    12361 actgaaaccg ccaatatttt acataacgcc accgaataca gtctggtgtt aatggatgag
    12421 atcgggcgtg gaacgtccac ctacgatggt ctgtcgctgg cgtgggcgtg cgcggaaaat
    12481 ctggcgaata agattaaggc attgacgtta tttgctaccc actatttcga gctgacccag
    12541 ttaccggaga aaatggaagg cgtcgctaac gtgcatctcg atgcactgga gcacggcgac
    12601 accattgcct ttatgcacag cgtgcaggat ggcgcggcga gcaaaagcta cggcctggcg
    12661 gttgcagctc tggcaggcgt gccaaaagag gttattaagc gcgcacggca aaagctgcgt
    12721 gagctggaaa gcatttcgcc gaacgccgcc gctacgcaag tggatggtac gcaaatgtct
    12781 ttgctgtcag taccagaaga aacttcgcct gcggtcgaag ctctggaaaa tcttgatccg
    12841 gattcactca ccccgcgtca ggcgctggag tggatttatc gcttgaagag cctggtgggt
    12901 tccggtaaca aaggacaggg a
//
