LOCUS       sequence-23143-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Zhou S, Zawel L, Lengauer C, Kinzler KW, Vogelstein B
  TITLE     Characterization of human FAST-1, a TGF beta and activin signal 
            transducer.
  JOURNAL   Mol Cell. 1998 Jul . 2(1):121-7.
  PUBMED    9702198
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            1998 Jul . 2(1):121-7."
COMMENT     Sequence Label: FBE/SBE-Luc
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      43..52
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    225..316
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(257..276)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    330..378
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(646..667)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cggangangg cgccgggcct ttctttatgt ttttggcgtc ttccatggtg gctttaccaa
       61 cagtaccgga atgccaagct tacttagatc gcagatctcg ataccggatc cattatatac
      121 ccgaattcga tatcgacgtc cagctgtcga gcccgggcta gcattgtcag tctagacata
      181 ctccgagctc ggtacagcca atacacaatc cggtacctat cgatagagaa atgttctggc
      241 acctgcactt gcactgggga cagcctattt tgctagtttg ttttgtttcg ttttgttttg
      301 atggagagcg tatgttagta ctatcgattc acacaaaaaa ccaacacaca gatgtaatga
      361 aaataaagat attttattgc ggccgctcca agtacctccc gtaccttaat attacttact
      421 tatcatggta gcttgggctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
      481 cagttgcgca gcctgaatgg cgaatggcaa attgtaagcg ttaatatttt gttaaaattc
      541 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
      601 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
      661 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgc naatcagggc
      721 gatggcccac tacgtgaacc tcaccctaat cangtttttt ggggtcgagg ggccgtaaag
      781 cactaaatgg gaaccctaaa agggagcccc cgattanagc t
//
