LOCUS       sequence-23144-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Zhou S, Zawel L, Lengauer C, Kinzler KW, Vogelstein B
  TITLE     Characterization of human FAST-1, a TGF beta and activin signal 
            transducer.
  JOURNAL   Mol Cell. 1998 Jul . 2(1):121-7.
  PUBMED    9702198
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            1998 Jul . 2(1):121-7."
COMMENT     Sequence Label: FBE-Luc
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      48..57
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    216..307
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(248..267)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    321..369
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(637..658)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tttcgcggat gatggcgccg ggcctttctt tatgtttttg gcgtcttcca tggtggcttt
       61 accaacagta ccggaatgcc aagcttactt agatcgcaga tctcgatacc ggatccatta
      121 tatacccgaa ttcgatatcg acgtccagct gtcgagcccg ggctagcacg cgtaagagct
      181 cggtacagcc aatacacaat ccggtaccta tcgatagaga aatgttctgg cacctgcact
      241 tgcactgggg acagcctatt ttgctagttt gttttgtttc gttttgtttt gatggagagc
      301 gtatgttagt actatcgatt cacacaaaaa accaacacac agatgtaatg aaaataaaga
      361 tattttattg cggccgctcc aagtacctcc cgtaccttaa tattacttac ttatcatggt
      421 agcttgggct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
      481 agcctgaatg gcgaatggca aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
      541 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
      601 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
      661 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
      721 ctacgtgaac cnncacccaa ancagttttt nggggncgag gngccgtaag cactaaatcg
      781 gancctnaag ggagccccga ttaganctgg acgggnaagc cgcnaacgtg gc
//
