LOCUS       sequence-23145-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Zhou S, Zawel L, Lengauer C, Kinzler KW, Vogelstein B
  TITLE     Characterization of human FAST-1, a TGF beta and activin signal 
            transducer.
  JOURNAL   Mol Cell. 1998 Jul . 2(1):121-7.
  PUBMED    9702198
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            1998 Jul . 2(1):121-7."
COMMENT     Sequence Label: SBE-Luc
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      45..54
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    206..297
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(238..257)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    311..359
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(627..648)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgcggaagan ggcgccgggc ctttctttat gtttttggcg tcttccatgg tggctttacc
       61 aacagtaccg gaatgccaag cttacttaga tcgcagatct cgataccgga tccattatat
      121 acccgaattc gatatcgacg tccagctgtc gagcccgggc tagcattgtc agtctagaca
      181 tactccgagc tcggtaccta tcgatagaga aatgttctgg cacctgcact tgcactgggg
      241 acagcctatt ttgctagttt gttttgtttc gttttgtttt gatggagagc gtatgttagt
      301 actatcgatt cacacaaaaa accaacacac agatgtaatg aaaataaaga tattttattg
      361 cggccgctcc aagtacctcc cgtaccttaa tattacttac ttatcatggt agcttgggct
      421 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      481 gcgaatggca aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
      541 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
      601 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
      661 tggactccaa cgtcaaaggg cgaaaaaccg tcnnggaggg ngatggccca ctaccggaac
      721 tgatcaccct aatcaagttt ttggggtcga ggtgccgtaa agcactaaat cggancctaa
      781 gggagccccg attagagctn gacgggaaag cggcnacgtg gcgagaagga aggaagaagc
      841 ga
//
