LOCUS       sequence-231450-        9296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual luciferase assay for miRNA-targeted UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9296)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 9296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: pmiRGLO-ASIC1
FEATURES             Location/Qualifiers
     source          1..9296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..523
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(59..82)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     456..475
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      564..573
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             570..2222
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    4336..4457
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4347..4366)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4401..4420
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        4702..5059
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4702..4893
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4725..4745)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4910..5045
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      5104..5113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5110..6042
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             6094..6885
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6145..6164)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6755..6774
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6949..6997
                     /note="synthetic polyadenylation signal"
     promoter        7153..7257
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7258..7326,7327..8118)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7476..7495)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(8276..8864)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8356..8375)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    8980..9263
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 ggcgtagagg atcgagatct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
       61 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
      121 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
      181 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
      241 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
      301 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
      361 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
      421 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
      481 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt
      541 ggcaatccgg tactgttggt aaagccacca tggaagatgc caaaaacatt aagaagggcc
      601 cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac aaagccatga
      661 agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc gaggtggaca
      721 ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg aagcgctatg
      781 ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag ttcttcatgc
      841 ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac atctacaacg
      901 agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc gtgagcaaga
      961 aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa aagatcatca
     1021 tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc gtgacttccc
     1081 atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac cgggacaaaa
     1141 ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc gtagccctac
     1201 cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc ggcaaccaga
     1261 tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc ggcatgttca
     1321 ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc ttcgaggagg
     1381 agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg gtgcccacac
     1441 tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc aacttgcacg
     1501 agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg gccaaacgct
     1561 tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc gccattctga
     1621 tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc ttcttcgagg
     1681 ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc ggcgagctgt
     1741 gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct acaaacgctc
     1801 tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac gaggacgagc
     1861 acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac caggtagccc
     1921 cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc ggggtcgccg
     1981 gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg gaacacggta
     2041 aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca accgccaaga
     2101 agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc ggcaagttgg
     2161 acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag atcgccgtgt
     2221 aattctagtt gtttaaacga gctcggcccc gcaggccgct gaaccaaagg cctagatggg
     2281 gaggactagg agagcgaggg ggcccccagc tgcctcctca catctgccct ggggactccc
     2341 cacactccgg ggcagatctt tcctcttgtc tgtggtaagg aaggagtctt gaccatagag
     2401 tcctctctct gcctctatcc cattcttttt acatttaaca aaactaatct aaaaaagaac
     2461 taaaaaggga gaacggggca agggacctca ggctgcccct ctctcctcca tgctgcctcc
     2521 cctagctccc agcctgaatt ctgtctatct agctgtctgc catctgagtg tccatctaca
     2581 ttctgctgcc accagtcacc aaaggccctt cccagtgagg ggtggaaggg atctctgggg
     2641 tctggaattt ggccccaaac cagagaatgt accttaaggg ggagggctag tgtgggggag
     2701 ggaggcttcc ccagccttaa gagaccctct cagcccagtg actgtcccca aacccaagtc
     2761 tcctggcagg aactaaaacc tcagccccac tctctcacac catgtggaat ctcgtggggg
     2821 tcggggatcc ccttaagaag tggtaatggg gacaagatgc ggccctggtg ctgtaggcta
     2881 catcctgata cctataagtt cacccccacc ccacagctgc tggagagaaa tcccaagagg
     2941 cagcccttcc tcaccatccc attaaagacc gggctggtta gcgtccagct cagggagaag
     3001 ggcgctagtg cctaacctca ctggtccctc tcccggaggc ccttgtagag ggccacgtcc
     3061 ataaattttc ttatggaact ctcccacatc ctcttcccca acttcatttg cttctctcaa
     3121 caacctcatc tgcattttct atttctatat gatacagact ctatattgct atatctctgt
     3181 atatactttc ccagccctgt ctgtctccac cccatcccct cttgtctctg agaaccattc
     3241 tcccacccca agttccacct tctatgtttc tactccctcc ctggtctctg aatgccttcg
     3301 cctgtataaa gagttggact ctctcccctg gtgtctgtac tgtgtacaca catccctctg
     3361 agaagcacaa ggagacgaca cgcgcattgt aacctttgca ctgtctcagt ggcgacaaag
     3421 gaagctgtga atcacaagct ctgcctcttt ctggcctcac cctctccccc aacccgggca
     3481 ccctcggccc tccctgcagc cttaacattc tcttcccctg ctcctcctat cccattgccc
     3541 tctgcccagc tgacagtggc atccccaggg aaggggttgc tgtagagata gcccccaccc
     3601 aggggatgga ggtctaccct ggacactaag ccaagtgtgt cagagacaga agggagctgg
     3661 ggattggcga ctcctgaagt tggggcagtg ggatgctgac aggcagaagc tgaggtcctc
     3721 agtcagtggc ctttcctcct tctgggtgcc cagccccctt tcctcacctg atacccaagc
     3781 ccaccacttt tattttctgg tgaggtgggt ttgggaggaa agagaggcct agaggaggag
     3841 ttgaaagctc tgctgttgtc tcaccctatc ttaatgagag acaagtgagg tggagggcct
     3901 gccccccctc cctccaccag acactccttc caggcctgag ccccaacccc tcttcaggcc
     3961 ttccttccct agctgtgtct tggtcttcaa tcccagaaca ggacctgtga gcagctgcat
     4021 tggcctggag ctggagagta aggctgtagg atctttggaa tctcttggtt cctaagagtt
     4081 tcctcagaga tcatacctcc ccagagggaa gcaggaatga ggccaaaaag tgtgcattgg
     4141 ataggggaac agcaggcagg gctctgggtg acgcatgcct ctggtctaat aaactgggtt
     4201 tcaaccaagt cgacctgcag gcatgcaagc tgatccggct gctaacaaag cccgaaagga
     4261 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcggccgc
     4321 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     4381 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     4441 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     4501 agatgtggga ggttttttta agcaagtaaa acctctacaa atgtggtaaa atcgaatttt
     4561 aacaaaatat taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg
     4621 gtatttcaca ccgcatacgc ggatctgcgc agcaccatgg cctgaaataa cctctgaaag
     4681 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     4741 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     4801 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4861 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     4921 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4981 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     5041 aggcctaggc ttttgcaaaa agcttgattc ttctgacaca acagtctcga accaaaggct
     5101 ggagccacca tggcttccaa ggtgtacgac cccgagcaac gcaaacgcat gatcactggg
     5161 cctcagtggt gggctcgctg caagcaaatg aacgtgctgg actccttcat caactactat
     5221 gattccgaga agcacgccga gaacgccgtg atttttctgc atggtaacgc tgcctccagc
     5281 tacctgtgga ggcacgtcgt gcctcacatc gagcccgtgg ctagatgcat catccctgat
     5341 ctgatcggaa tgggtaagtc cggcaagagc gggaatggct catatcgcct cctggatcac
     5401 tacaagtacc tcaccgcttg gttcgagctg ctgaaccttc caaagaaaat catctttgtg
     5461 ggccacgact ggggggcttg tctggccttt cactactcct acgagcacca agacaagatc
     5521 aaggccatcg tccatgctga gagtgtcgtg gacgtgatcg agtcctggga cgagtggcct
     5581 gacatcgagg aggatatcgc cctgatcaag agcgaagagg gcgagaaaat ggtgcttgag
     5641 aataacttct tcgtcgagac catgctccca agcaagatca tgcggaaact ggagcctgag
     5701 gagttcgctg cctacctgga gccattcaag gagaagggcg aggttagacg gcctaccctc
     5761 tcctggcctc gcgagatccc tctcgttaag ggaggcaagc ccgacgtcgt ccagattgtc
     5821 cgcaactaca acgcctacct tcgggccagc gacgatctgc ctaagatgtt catcgagtcc
     5881 gaccctgggt tcttttccaa cgctattgtc gagggagcta agaagttccc taacaccgag
     5941 ttcgtgaagg tgaagggcct ccacttcagc caggaggacg ctccagatga aatgggtaag
     6001 tacatcaaga gcttcgtgga gcgcgtgctg aagaacgagc agaccggtgg tgggagcgga
     6061 ggtggcggat caggtggcgg aggctccgga gggattgaac aagatggatt gcacgcaggt
     6121 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     6181 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     6241 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     6301 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     6361 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     6421 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     6481 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     6541 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     6601 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     6661 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     6721 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     6781 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     6841 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     6901 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg atggccgcaa taaaatatct
     6961 ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagcgat aaggatcctc
     7021 tttgcgcttg cgttttccct tgtccagata gcccagtagc tgacattcat ccggggtcag
     7081 caccgtttct gcggactggc tttctacgta atggtttctt agacgtcagg tggcactttt
     7141 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     7201 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     7261 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     7321 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     7381 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     7441 gaacgttttc caatgatgag cactttcaaa gttctgctat gtggcgcggt attatcccgt
     7501 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     7561 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     7621 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aactatcgga
     7681 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     7741 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     7801 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     7861 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     7921 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     7981 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     8041 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     8101 ctgattaagc attggtaatt cgaaatgacc gaccaagcga cgcccaaccg gtatcagctc
     8161 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     8221 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     8281 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8341 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8401 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8461 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8521 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8581 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8641 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8701 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8761 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8821 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatt tcaagaagat cctttgatct
     8881 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8941 gattatcaaa aaggatcttc acctagatcc ttttatagtc cggaaataca ggaacgcacg
     9001 ctggatggcc cttcgctggg atggtgaaac catgaaaaat ggcagcttca gtggattaag
     9061 tgggggtaat gtggcctgta ccctctggtt gcataggtat tcatacggtt aaaatttatc
     9121 aggcgcgatt gcggcagttt ttcgggtggt ttgttgccat ttttacctgt ctgctgccgt
     9181 gatcgcgctg aacgcgtttt agcggtgcgt acaattaagg gattatggta aatccactta
     9241 ctgtctgccc tcgtagccat cgagataaac cgcagtactc cggccacgat gcgtcc
//
