LOCUS       sequence-231459-        5395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Gordon J, Bennett AR, Blackburn CC, Manley NR
  TITLE     Gcm2 and Foxn1 mark early parathyroid- and thymus-specific domains 
            in the developing third pharyngeal pouch.
  JOURNAL   Mech Dev. 2001 May;103(1-2):141-3.
  PUBMED    11335122
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mech Dev.";
            date: "2001-05"; volume: "103(1-2)"; pages: "141-3"
COMMENT     Sequence Label: Foxn1-pBSII SK
FEATURES             Location/Qualifiers
     source          1..5395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     44..60
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     45..61
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(95..111)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(95..111)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2582..2602)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2582..2600)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2621..2637)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2621..2637)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2634..2656)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2645..2661
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2669..2675,2676..2693,2694..2699))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2714..2735
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2852..2869)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3023..3611)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3103..3122)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3782..4573,4574..4642))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4405..4424
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4643..4747)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4773..5228)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4910..4931)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5122..5141
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5355..5377
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5369..5386
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5370..5386
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt cgacggtatc
       61 gataagcttg atatcgaatt cctgcagccc gggggatcca ctagttctag agcggccgct
      121 gccaggactg ggtgatggtg tcgctactcc ctccgcagtc tgacgtcaca cttccaggct
      181 ccacccgact ggagggcgaa ccccaagggg acctcatgca ggctccgggc ctcccagact
      241 cccctgcccc acagaacaag catgctaact tcagctgctc gtcgtttgtg cctgacggcc
      301 ctccagagag gacaccctca ctgcccccac acagccccag catcgcatct ccagacccag
      361 agcagatcca gggccactgc acagccggac ccggcccggg ctccttccgc ctttctcctt
      421 cagaaaagta tcctggcttt ggctttgagg agggcccagc aggcagccca gggcgcttcc
      481 tcaagggcaa ccacatgcct ttccacccct acaagaggca cttccatgag gacatcttct
      541 ctgaggccca gacggccatg gcacttgatg gacactcctt taagactcag ggggcactgg
      601 aagcctttga ggagatccct gtggacatgg gcgatgctga ggccttcctg cctagcttcc
      661 cagcagaggc ttggtgcaat aaactccctt accccagcca ggaacacaac caaattctgc
      721 aggggtcaga ggtcaaggtc aagccccaag ctctggacag tggtcctggg atgtactgct
      781 accagcctcc cttgcaacat atgtactgtt cttctcagcc tgccttccat cagtactccc
      841 cgggtggagg cagctaccct gtgccctacc tgggctcacc tcactatccc tatcagagga
      901 ttgcacccca ggccaacgcc gaaggtcacc agccactctt cccaaagccc atctactctt
      961 acagcatcct catcttcatg gcccttaaga acagtaagac cggaagcctt ccagtcagtg
     1021 aaatctacaa tttcatgacg gagcacttcc cttacttcaa gactgctcct gatggctgga
     1081 agaattctgt tcgccataac ctgtccctca acaagtgctt tgagaaggtg gagaataaat
     1141 ccggaagttc ctctcgaaag ggctgtctgt gggccctcaa tccttccaaa atcgacaaga
     1201 tgcaggaaga actgcagaag tggaagagga aagaccccat tgctgtgcgc aaaagcatgg
     1261 ccaaaccaga agagctggac agcctcattg gagacaaaag ggaaaaactg ggctctccgc
     1321 tgctgggctg tccaccccct gggctggcag gcccaggtcc catccggccc atggcaccat
     1381 cagctggtct ttcccagcct ctgcacccaa tgcatccagc tccaggcccc atgcctggca
     1441 agaaccccct gcaggaccta ctgggtggcc atgctccctc ctgctatggg cagacctacc
     1501 cacacctttc ccccagcctg gccccttctg gacaccagca gccattgttc ccacagccag
     1561 atgggcatct tgagctgcag gcccagccag gcacccccca ggactcacct ctacctgccc
     1621 acacaccacc cagccacggt gccaagctga tggctgagcc ttcctcagcc aggaccatgc
     1681 acgatactct actgccagat ggagaccttg ggactgacct ggatgctatc aacccttctc
     1741 tcactgactt cgacttccag ggaaatctgt gggagcagct gaaggatgac agcttggccc
     1801 tggaccccct cgtattggtg acctcgtcgc cgacgtcatc ctccatgttg ccacccccac
     1861 cagcagccca ttgcttcccc ccagggcctt gtctggcaga aacaggcaat gaggcaggtg
     1921 aactggcacc tccaggcagc ggcggctccg gtgctctggg agacatgcac ctcagcactc
     1981 tctactccgc ctttgtggaa ctggagtcca cgccctcctc agcagctgcc ggccctgccg
     2041 tgtacctcag tcccggctca aagccattgg ctctggcttg agctatgccc aacatcagct
     2101 gcggcctttg cctagctggc tgccccatat tgggctcacc ttaaaggtca aagaaggaaa
     2161 acactacctg tctcctatgc cactcagcca acttatttgt tagccagaag ttgggggcat
     2221 ccatctagga tgctacctgg tggacccgca tctcacacgg ggcccccagc aaggaaagtg
     2281 tcggaaaaga agcaacagcg cgccccctag cgccagctca ctcgaacttc agctctcgag
     2341 cgtgaactca agctctacac acctgctcat catgtgcctt acactcagag gaagccttgg
     2401 gtcacagagt ctgatttgat ttctggggca gctgagagct aaaagcttta gttagcaaag
     2461 ctcagggcca gtgtgacagg tcaaagatca cccctccaaa cctcatccca atccccatgt
     2521 gctctacaga cttggccctc tcccagcggc cgccaccgcg gtggagctcc agcttttgtt
     2581 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     2641 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     2701 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     2761 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     2821 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2881 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2941 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     3001 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3061 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3121 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3181 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3241 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3301 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3361 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3421 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3481 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3541 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3601 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3661 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3721 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3781 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     3841 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     3901 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     3961 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     4021 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     4081 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     4141 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     4201 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     4261 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     4321 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     4381 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     4441 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     4501 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     4561 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     4621 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     4681 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4741 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
     4801 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     4861 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     4921 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     4981 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     5041 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     5101 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     5161 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     5221 gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     5281 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     5341 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcg
//
