LOCUS       sequence-231465-       10793 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BRAF T599_V600insP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10793)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-BRAF-T599_V600insP
FEATURES             Location/Qualifiers
     source          1..10793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3446..3455
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(5753..5777)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5785..5803
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5828..5847)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5829..5847)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5853..5873)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5927..5968
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5989..6556
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6006..6558
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6150..6167)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6377..6396
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6570..7169
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6570..6589)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7066..7086
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7183..7771
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7236..7256)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7654..7665)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7846..8079
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8198..8216)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8284..8388
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8389..8457,8458..9249)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8607..8626)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9420..10008
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9909..9928
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10162..10179
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10419..10440
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10455..10460,10461..10478,10479..10485)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10493..10509
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10498..10520
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10517..10533
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10517..10533
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10583..10602
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg gcggcgctga gcggtggcgg tggtggcggc
     3481 gcggagccgg gccaggctct gttcaacggg gacatggagc ccgaggccgg cgccggcgcc
     3541 ggcgccgcgg cctcttcggc tgcggaccct gccattccgg aggaggtgtg gaatatcaaa
     3601 caaatgatta agttgacaca ggaacatata gaggccctat tggacaaatt tggtggggag
     3661 cataatccac catcaatata tctggaggcc tatgaagaat acaccagcaa gctagatgca
     3721 ctccaacaaa gagaacaaca gttattggaa tctctgggga acggaactga tttttctgtt
     3781 tctagctctg catcaatgga taccgttaca tcttcttcct cttctagcct ttcagtgcta
     3841 ccttcatctc tttcagtttt tcaaaatccc acagatgtgg cacggagcaa ccccaagtca
     3901 ccacaaaaac ctatcgttag agtcttcctg cccaacaaac agaggacagt ggtacctgca
     3961 aggtgtggag ttacagtccg agacagtcta aagaaagcac tgatgatgag aggtctaatc
     4021 ccagagtgct gtgctgttta cagaattcag gatggagaga agaaaccaat tggttgggac
     4081 actgatattt cctggcttac tggagaagaa ttgcatgtgg aagtgttgga gaatgttcca
     4141 cttacaacac acaactttgt acgaaaaacg tttttcacct tagcattttg tgacttttgt
     4201 cgaaagctgc ttttccaggg tttccgctgt caaacatgtg gttataaatt tcaccagcgt
     4261 tgtagtacag aagttccact gatgtgtgtt aattatgacc aacttgattt gctgtttgtc
     4321 tccaagttct ttgaacacca cccaatacca caggaagagg cgtccttagc agagactgcc
     4381 ctaacatctg gatcatcccc ttccgcaccc gcctcggact ctattgggcc ccaaattctc
     4441 accagtccgt ctccttcaaa atccattcca attccacagc ccttccgacc agcagatgaa
     4501 gatcatcgaa atcaatttgg gcaacgagac cgatcctcat cagctcccaa tgtgcatata
     4561 aacacaatag aacctgtcaa tattgatgac ttgattagag accaaggatt tcgtggtgat
     4621 ggaggatcaa ccacaggttt gtctgctacc ccccctgcct cattacctgg ctcactaact
     4681 aacgtgaaag ccttacagaa atctccagga cctcagcgag aaaggaagtc atcttcatcc
     4741 tcagaagaca ggaatcgaat gaaaacactt ggtagacggg actcgagtga tgattgggag
     4801 attcctgatg ggcagattac agtgggacaa agaattggat ctggatcatt tggaacagtc
     4861 tacaagggaa agtggcatgg tgatgtggca gtgaaaatgt tgaatgtgac agcacctaca
     4921 cctcagcagt tacaagcctt caaaaatgaa gtaggagtac tcaggaaaac acgacatgtg
     4981 aatatcctac tcttcatggg ctattccaca aagccacaac tggctattgt tacccagtgg
     5041 tgtgagggct ccagcttgta tcaccatctc catatcattg agaccaaatt tgagatgatc
     5101 aaacttatag atattgcacg acagactgca cagggcatgg attacttaca cgccaagtca
     5161 atcatccaca gagacctcaa gagtaataat atatttcttc atgaagacct cacagtaaaa
     5221 ataggtgatt ttggtctagc tacaccagtg aaatctcgat ggagtgggtc ccatcagttt
     5281 gaacagttgt ctggatccat tttgtggatg gcaccagaag tcatcagaat gcaagataaa
     5341 aatccataca gctttcagtc agatgtatat gcatttggaa ttgttctgta tgaattgatg
     5401 actggacagt taccttattc aaacatcaac aacagggacc agataatttt tatggtggga
     5461 cgaggatacc tgtctccaga tctcagtaag gtacggagta actgtccaaa agccatgaag
     5521 agattaatgg cagagtgcct caaaaagaaa agagatgaga gaccactctt tccccaaatt
     5581 ctcgcctcta ttgagctgct ggcccgctca ttgccaaaaa ttcaccgcag tgcatcagaa
     5641 ccctccttga atcgggctgg tttccaaaca gaggatttta gtctatatgc ttgtgcttct
     5701 ccaaaaacac ccatccaggc agggggatat ggtgcgtttc ctgtccacta gaacccagct
     5761 ttcttgtaca aagtggtgat atccaattaa ccctcactaa aggtagataa tgtgaagtaa
     5821 taagtgaccc tatagtgagt cgtattacca cccaactttt ctatacaaag tggttgatat
     5881 ccagcacagt ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta agcctatccc
     5941 taaccctctc ctcggtctcg attctacgcg taccggttag taatgagatc cctccccccc
     6001 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     6061 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     6121 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     6181 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     6241 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     6301 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     6361 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     6421 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     6481 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     6541 aaaaacacga tgataatatg gccacacata tgaccgagta caagcccacg gtgcgcctcg
     6601 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     6661 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     6721 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     6781 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     6841 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     6901 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     6961 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     7021 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     7081 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     7141 ggtgcatgac ccgcaagccc ggtgcctaaa tcgatagatc ctaatcaacc tctggattac
     7201 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     7261 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     7321 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     7381 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     7441 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     7501 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     7561 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     7621 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     7681 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     7741 agtcggatct ccctttgggc cgcctccccg cctgagatcc tttaagacca atgacttaca
     7801 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     7861 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     7921 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7981 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8041 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     8101 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg cccgggttaa
     8161 ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     8221 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     8281 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     8341 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     8401 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     8461 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     8521 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     8581 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     8641 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     8701 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     8761 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     8821 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     8881 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     8941 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     9001 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     9061 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     9121 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     9181 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     9241 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     9301 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     9361 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     9421 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     9481 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     9541 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     9601 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     9661 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     9721 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     9781 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     9841 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     9901 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     9961 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    10021 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    10081 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    10141 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
    10201 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagcaagct catggctgac
    10261 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
    10321 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctccccgtg gcacgacagg
    10381 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
    10441 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
    10501 ggataacaat ttcacacagg aaacagctat gacatgatta cgaatttcac aaataaagca
    10561 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    10621 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct
    10681 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta
    10741 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agt
//
