LOCUS       sequence-231489-        9703 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual luciferase assay for miRNA-targeted UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9703)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 9703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: pmiRGLO-CAMK2B-209
FEATURES             Location/Qualifiers
     source          1..9703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..509
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(45..68)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     442..461
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      550..559
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             556..2208
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    4729..4850
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4740..4759)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4794..4813
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5095..5452
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5095..5286
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5118..5138)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5303..5438
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      5497..5506
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5503..6435
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             6487..7278
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6538..6557)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7148..7167
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7342..7390
                     /note="synthetic polyadenylation signal"
     promoter        7546..7650
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7651..7719,7720..8511)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7869..7888)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(8669..9257)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8749..8768)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    9373..9656
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 agatctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
       61 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
      121 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
      181 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
      241 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
      301 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
      361 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
      421 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
      481 tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttggca atccggtact
      541 gttggtaaag ccaccatgga agatgccaaa aacattaaga agggcccagc gccattctac
      601 ccactcgaag acgggaccgc cggcgagcag ctgcacaaag ccatgaagcg ctacgccctg
      661 gtgcccggca ccatcgcctt taccgacgca catatcgagg tggacattac ctacgccgag
      721 tacttcgaga tgagcgttcg gctggcagaa gctatgaagc gctatgggct gaatacaaac
      781 catcggatcg tggtgtgcag cgagaatagc ttgcagttct tcatgcccgt gttgggtgcc
      841 ctgttcatcg gtgtggctgt ggccccagct aacgacatct acaacgagcg cgagctgctg
      901 aacagcatgg gcatcagcca gcccaccgtc gtattcgtga gcaagaaagg gctgcaaaag
      961 atcctcaacg tgcaaaagaa gctaccgatc atacaaaaga tcatcatcat ggatagcaag
     1021 accgactacc agggcttcca aagcatgtac accttcgtga cttcccattt gccacccggc
     1081 ttcaacgagt acgacttcgt gcccgagagc ttcgaccggg acaaaaccat cgccctgatc
     1141 atgaacagta gtggcagtac cggattgccc aagggcgtag ccctaccgca ccgcaccgct
     1201 tgtgtccgat tcagtcatgc ccgcgacccc atcttcggca accagatcat ccccgacacc
     1261 gctatcctca gcgtggtgcc atttcaccac ggcttcggca tgttcaccac gctgggctac
     1321 ttgatctgcg gctttcgggt cgtgctcatg taccgcttcg aggaggagct attcttgcgc
     1381 agcttgcaag actataagat tcaatctgcc ctgctggtgc ccacactatt tagcttcttc
     1441 gctaagagca ctctcatcga caagtacgac ctaagcaact tgcacgagat cgccagcggc
     1501 ggggcgccgc tcagcaagga ggtaggtgag gccgtggcca aacgcttcca cctaccaggc
     1561 atccgccagg gctacggcct gacagaaaca accagcgcca ttctgatcac ccccgaaggg
     1621 gacgacaagc ctggcgcagt aggcaaggtg gtgcccttct tcgaggctaa ggtggtggac
     1681 ttggacaccg gtaagacact gggtgtgaac cagcgcggcg agctgtgcgt ccgtggcccc
     1741 atgatcatga gcggctacgt taacaacccc gaggctacaa acgctctcat cgacaaggac
     1801 ggctggctgc acagcggcga catcgcctac tgggacgagg acgagcactt cttcatcgtg
     1861 gaccggctga agagcctgat caaatacaag ggctaccagg tagccccagc cgaactggag
     1921 agcatcctgc tgcaacaccc caacatcttc gacgccgggg tcgccggcct gcccgacgac
     1981 gatgccggcg agctgcccgc cgcagtcgtc gtgctggaac acggtaaaac catgaccgag
     2041 aaggagatcg tggactatgt ggccagccag gttacaaccg ccaagaagct gcgcggtggt
     2101 gttgtgttcg tggacgaggt gcctaaagga ctgaccggca agttggacgc ccgcaagatc
     2161 cgcgagattc tcattaaggc caagaagggc ggcaagatcg ccgtgtaatt ctagttgttt
     2221 aaacgagctc gagctgcgcc ctggtttcgc cggacagagt tggtgtttgg agcccgactg
     2281 ccctcgggca cacggcctgc ctgtcgcatg tttgtgtctg cctcgttccc tcccctggtg
     2341 cctgtgtctg cagaaaaaca agaccagatg tgatttgtta aaaaaaaaca aaaaaaaaaa
     2401 aaaaaaaanc aagatgacga cgacaaccac aaaaaaaatt gacatcagat gaaatgaaaa
     2461 aaaaaaaaaa aacaaaaaaa actaaaggaa ggaaaaagct gtaaaaatca ctggcattcg
     2521 tggggccact ccccacccaa gctccacgtg tgtccgtctg tgctcctggc ctctggggga
     2581 ccagctggga catgaacttg tctgccaggc ccccgtcgcg tgctgaacgg tgttagtttg
     2641 taggtaacgc acacacccca cacctaaggt gtctgcatcc tcctgccaac gcatgggctc
     2701 cacgtggtgt gctcgctggc tgtcgtgact gtcagctgtc tcttgggagg ggctgtgggg
     2761 gcccgctggg ctgcctcctt tcccgctagt tgtgcctgag agttgctgtt gttcctgctt
     2821 tcccttccct tcctttcatc ccctgaaggg ctaggtgtgg gttttccgtg cccggtatcc
     2881 ccacacaccc agcacggaca acccttcggc agagcccagg ccggcccctc accccctgga
     2941 gtattgaaac tggagtcccg tccccaaggc cttcagagat gcccctacac acccagggct
     3001 ccagctctgg tccttctggg ggagtaaagt gcaaagaggg gcacagctta gttttgggcc
     3061 tctcgccgag caagagacag cactgctggc tacagctcca acacagccag ctgtggcaag
     3121 aggactctgc ctgggctggc ccccctcctg tgtgaggtgt ctgtcccttc tctgctggcc
     3181 agcagcagat gcactggcag ctcccaaccc tgtttccgcc cctcggccct cccccagcct
     3241 gttcggcttc tctgcagccc gcaaggggga gcagactttt gacaaaggac tgcgggcctc
     3301 gctcaagtcc ctgagccccc agctgaagct gggaggggag gccaggcttt gtgtctgggc
     3361 atattcgtct gctgatgggg tttggggaag cctggggctt ggggtttggt cgggtggtgc
     3421 agctagtggc agagcgggat cagaggtggt ggctgcccag cttctgggct gagacaaggg
     3481 tctgtgcagg ggtttactga agtgggagtg cctttggaat ctgggccggg agcagaaggg
     3541 agcaaaagct acagtgggag ccagcctagg gcacatggga ggcgtgaggg cagtgctgcc
     3601 cgtgcagtgt caggtgtgcc agtgccttgg cgggctgcag tgcgtgtgag ggcaccttct
     3661 aggtgggcca gggatgcagc tatggagata aggcgggctg gggacagaaa caggtgggca
     3721 cagggcccag gacaccagcg gatggagggc agggtctagc cctgtgctcc tgagcgtcgg
     3781 ctgcctgggt tcgaggcggt gggtccccgg ccccttgtga tggtgtgtac catgggggag
     3841 ctcggggaca gggcaagccc gagcatggtg gggctgcagg gtgggtctga agccaggttg
     3901 ggtgggggtg gtcacaagcc ctgactgcag agggtcaggg gctcctgccc cagtgcctgc
     3961 ccactttcaa ttcacattgt tttcaacaag gattttcttt atcttcccct acaaatcaag
     4021 ccaagggagg ggcacagaat ggggaacagg acacaggatc ctaaactcca aggggactgt
     4081 ccaccgatga acactcagag tggacaccat cttccgtcca cgctgtgccc aggacagctg
     4141 tccccatcca tgaacacagg gtaaacatct gccgggctcc gcaccagtgg ctccctgggc
     4201 catgggacag cggcagggct caccacggac agcacgtggc ccagcagccg gccaccctgg
     4261 cgtcctgggg cctcctcccc tcctctccct ctcaccttgt cacctccacg gagctgcctg
     4321 tctgggataa tttggggatt ttttttctgg gggataattc ttttgcatga cccctaaaga
     4381 gcaagccaca ccggtctgct agctaggtgt ccgcggtgtg gtggtggcgg ccgctggcca
     4441 gcgctgcaag gggtcggctg cccacggtgc tggctggcct cccctcctct ctctttttgc
     4501 tgagtttcat tgtcttttct ttctgagcct tgtaagtgta caaaaattat tcttattttg
     4561 ttctgtctcg ggaaactgca aataaaagaa aaacaggaca agtcgacctg caggcatgca
     4621 agctgatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
     4681 gcaataacta gcataacccc ttggggcggc cgcttcgagc agacatgata agatacattg
     4741 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     4801 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     4861 attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt ttaagcaagt
     4921 aaaacctcta caaatgtggt aaaatcgaat tttaacaaaa tattaacgct tacaatttcc
     4981 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgcggatctg
     5041 cgcagcacca tggcctgaaa taacctctga aagaggaact tggttaggta ccttctgagg
     5101 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     5161 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     5221 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     5281 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     5341 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     5401 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttga
     5461 ttcttctgac acaacagtct cgaaccaaag gctggagcca ccatggcttc caaggtgtac
     5521 gaccccgagc aacgcaaacg catgatcact gggcctcagt ggtgggctcg ctgcaagcaa
     5581 atgaacgtgc tggactcctt catcaactac tatgattccg agaagcacgc cgagaacgcc
     5641 gtgatttttc tgcatggtaa cgctgcctcc agctacctgt ggaggcacgt cgtgcctcac
     5701 atcgagcccg tggctagatg catcatccct gatctgatcg gaatgggtaa gtccggcaag
     5761 agcgggaatg gctcatatcg cctcctggat cactacaagt acctcaccgc ttggttcgag
     5821 ctgctgaacc ttccaaagaa aatcatcttt gtgggccacg actggggggc ttgtctggcc
     5881 tttcactact cctacgagca ccaagacaag atcaaggcca tcgtccatgc tgagagtgtc
     5941 gtggacgtga tcgagtcctg ggacgagtgg cctgacatcg aggaggatat cgccctgatc
     6001 aagagcgaag agggcgagaa aatggtgctt gagaataact tcttcgtcga gaccatgctc
     6061 ccaagcaaga tcatgcggaa actggagcct gaggagttcg ctgcctacct ggagccattc
     6121 aaggagaagg gcgaggttag acggcctacc ctctcctggc ctcgcgagat ccctctcgtt
     6181 aagggaggca agcccgacgt cgtccagatt gtccgcaact acaacgccta ccttcgggcc
     6241 agcgacgatc tgcctaagat gttcatcgag tccgaccctg ggttcttttc caacgctatt
     6301 gtcgagggag ctaagaagtt ccctaacacc gagttcgtga aggtgaaggg cctccacttc
     6361 agccaggagg acgctccaga tgaaatgggt aagtacatca agagcttcgt ggagcgcgtg
     6421 ctgaagaacg agcagaccgg tggtgggagc ggaggtggcg gatcaggtgg cggaggctcc
     6481 ggagggattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     6541 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     6601 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     6661 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     6721 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     6781 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     6841 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     6901 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     6961 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     7021 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     7081 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     7141 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     7201 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     7261 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     7321 acctgccatc acgatggccg caataaaata tctttatttt cattacatct gtgtgttggt
     7381 tttttgtgtg aatcgatagc gataaggatc ctctttgcgc ttgcgttttc ccttgtccag
     7441 atagcccagt agctgacatt catccggggt cagcaccgtt tctgcggact ggctttctac
     7501 gtaatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     7561 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     7621 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     7681 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     7741 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     7801 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttc
     7861 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     7921 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     7981 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     8041 actgcggcca acttacttct gacaactatc ggaggaccga aggagctaac cgcttttttg
     8101 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     8161 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     8221 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     8281 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     8341 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     8401 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     8461 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta attcgaaatg
     8521 accgaccaag cgacgcccaa ccggtatcag ctcactcaaa ggcggtaata cggttatcca
     8581 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     8641 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8701 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8761 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8821 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8881 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8941 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     9001 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9061 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     9121 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9181 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9241 gaaaaaaagg atttcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9301 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     9361 tccttttata gtccggaaat acaggaacgc acgctggatg gcccttcgct gggatggtga
     9421 aaccatgaaa aatggcagct tcagtggatt aagtgggggt aatgtggcct gtaccctctg
     9481 gttgcatagg tattcatacg gttaaaattt atcaggcgcg attgcggcag tttttcgggt
     9541 ggtttgttgc catttttacc tgtctgctgc cgtgatcgcg ctgaacgcgt tttagcggtg
     9601 cgtacaatta agggattatg gtaaatccac ttactgtctg ccctcgtagc catcgagata
     9661 aaccgcagta ctccggccac gatgcgtccg gcgtagagga tcg
//
