LOCUS       sequence-231491-        9947 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual luciferase assay for miRNA-targeted UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9947)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 9947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: pmiRGLO-FAM102A-203
FEATURES             Location/Qualifiers
     source          1..9947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        76..575
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(111..134)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     508..527
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      616..625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             622..2274
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    5039..5160
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5050..5069)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5104..5123
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5405..5762
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5405..5596
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5428..5448)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5613..5748
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      5807..5816
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5813..6745
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             6797..7588
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6848..6867)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7458..7477
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7652..7700
                     /note="synthetic polyadenylation signal"
     promoter        7856..7960
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7961..8029,8030..8821)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8179..8198)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(8979..9567)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9059..9078)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(9683..9947,1..19)
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 ctgccctcgt agccatcgag ataaaccgca gtactccggc cacgatgcgt ccggcgtaga
       61 ggatcgagat ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
      121 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
      181 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
      241 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
      301 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
      361 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
      421 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
      481 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
      541 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttggcaatcc
      601 ggtactgttg gtaaagccac catggaagat gccaaaaaca ttaagaaggg cccagcgcca
      661 ttctacccac tcgaagacgg gaccgccggc gagcagctgc acaaagccat gaagcgctac
      721 gccctggtgc ccggcaccat cgcctttacc gacgcacata tcgaggtgga cattacctac
      781 gccgagtact tcgagatgag cgttcggctg gcagaagcta tgaagcgcta tgggctgaat
      841 acaaaccatc ggatcgtggt gtgcagcgag aatagcttgc agttcttcat gcccgtgttg
      901 ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg acatctacaa cgagcgcgag
      961 ctgctgaaca gcatgggcat cagccagccc accgtcgtat tcgtgagcaa gaaagggctg
     1021 caaaagatcc tcaacgtgca aaagaagcta ccgatcatac aaaagatcat catcatggat
     1081 agcaagaccg actaccaggg cttccaaagc atgtacacct tcgtgacttc ccatttgcca
     1141 cccggcttca acgagtacga cttcgtgccc gagagcttcg accgggacaa aaccatcgcc
     1201 ctgatcatga acagtagtgg cagtaccgga ttgcccaagg gcgtagccct accgcaccgc
     1261 accgcttgtg tccgattcag tcatgcccgc gaccccatct tcggcaacca gatcatcccc
     1321 gacaccgcta tcctcagcgt ggtgccattt caccacggct tcggcatgtt caccacgctg
     1381 ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga ggagctattc
     1441 ttgcgcagct tgcaagacta taagattcaa tctgccctgc tggtgcccac actatttagc
     1501 ttcttcgcta agagcactct catcgacaag tacgacctaa gcaacttgca cgagatcgcc
     1561 agcggcgggg cgccgctcag caaggaggta ggtgaggccg tggccaaacg cttccaccta
     1621 ccaggcatcc gccagggcta cggcctgaca gaaacaacca gcgccattct gatcaccccc
     1681 gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga ggctaaggtg
     1741 gtggacttgg acaccggtaa gacactgggt gtgaaccagc gcggcgagct gtgcgtccgt
     1801 ggccccatga tcatgagcgg ctacgttaac aaccccgagg ctacaaacgc tctcatcgac
     1861 aaggacggct ggctgcacag cggcgacatc gcctactggg acgaggacga gcacttcttc
     1921 atcgtggacc ggctgaagag cctgatcaaa tacaagggct accaggtagc cccagccgaa
     1981 ctggagagca tcctgctgca acaccccaac atcttcgacg ccggggtcgc cggcctgccc
     2041 gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg taaaaccatg
     2101 accgagaagg agatcgtgga ctatgtggcc agccaggtta caaccgccaa gaagctgcgc
     2161 ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt ggacgcccgc
     2221 aagatccgcg agattctcat taaggccaag aagggcggca agatcgccgt gtaattctag
     2281 ttgtttaaac gagctcggga gcaggtgtcc gggctggaga agagtcctgc tttctctgga
     2341 gtccagacct gtatcattcc atgaggaact ttccccttca gatcacctct gcgccacatc
     2401 tcatccatgc ctcctccatg cactccagtc cacactcccc gtagcatcat tccattgccc
     2461 ctcccatcca tgctgggacc ctcctggccc accaaggccc aggcaccact gtgaatattc
     2521 tcctctgaac cactagaggg caggccaggc aggccaggcg ggcccgtgca gcttgtgggc
     2581 aagaaggagc tggcaaggac cggcgctgct ggagactgac ccagccctct ggctgaggac
     2641 atgcagcggc tcctaaatgt agagatgcct gtggctgagg gggcctctct acctgtgtcc
     2701 ccactcactc caggagcact ggctttggtc acgtcttagc agcagggcct tgctccgttg
     2761 ttcccttgcc ctggtggtgg gggggccaga ccacctccgg aatcctgcca cctgtgactg
     2821 tctgactgct tagtgcttca gctgtccctt ccttgtgtcc tgggggacct gctggcggcc
     2881 tcttcctggg agccatgacc tcagacccca cccacactcc agatcgagac ccctgcctcc
     2941 ccccggcaaa tgtcctcccg ctgccttgca gcctgcactt tgcacatgct cacccccagc
     3001 acagtcccac tggcccctca cctccccttc cctgagctcc ttcccaagga ctcctggtca
     3061 ctgcctgctg tgcagtcaga ggcccagggt ccagcagccc ggcgggaacg ggtgctgcct
     3121 cttcctccag ttagctccag ctcaggtctg agacccgtgc tgagaaaggt ctgagcaccg
     3181 accgtgccct ctgcccaggg ctgggtcctg agcagctggt tttcctgcag gaaggttgga
     3241 gcaagcaaag tccttctctg ccctcagggt cagctgccca gactggggcg gatgccagag
     3301 aggcaggtgg gctgtggctg gactggtccg gagctggctt ccttaccaga aaagcctcag
     3361 ccttcctctg gaagcatccc ccgttctggg caagggggaa gggctccttt aaggggtgtg
     3421 ctttcccagt ggggagcagt ctggccctgc cccctactaa agcctctgct ctcagcactt
     3481 tcccccaagt ccttgtaact tgcttgaagg tgggttctgg ctgccagcca gtccctggac
     3541 aaactctcct gcccctttta aatttcactc attttgtata aacccagcag gctggtgttt
     3601 acttagccct gtagcttttt tcattttttc tttccgtctt tcttcttgag ttcacggttc
     3661 aatattgcct cctcgccctg gtgaggggag gtgctgcttt tctgccccac ctgccggctg
     3721 gttccagcag cgctggggcc cagctggggg gccgggatgg gggcttctct ctctgggagg
     3781 ggtgcaggtg ccctccccag gctgggaggg ttccttccct agctccccat ctgcccccgc
     3841 tggtgagagt tgggcttctt ggtcttggaa ctccctggca ttgggaacag agcatttcca
     3901 gcatttgttg ttgttttact cacctaaccc ttagaaaatg aatgttagaa ggtgcctgcc
     3961 gaggcgggac agagtgttcg ctcgcgctgg agaaggctct gctcagccct gagagtccct
     4021 tcctgcccca ccgatactgg cactttaaaa aggaagctga ccgcacagtg tccagacgaa
     4081 ttggccccca gaagatgggg agttctgtcc tgcccttctg tgtctgcgtg acctcaccca
     4141 gcctaggagg gaggtgcatt cagggtagat ttgcctctca ttcaaagttc tggggctttg
     4201 ggtggaaaac agccagcttt ggcgctgttg gggagactcc tccagaccag gaaccccaga
     4261 aggagacaga gcctgccaca tcctcccacg ccaggccctg ggccagggtg attggactga
     4321 gaatttggcc acaaccaaat tgatgctggc tggaaccaga ggccagaaag cctggccttg
     4381 tccccatgtg ggagccctgt cctcagccct cttgtcccct tgagctcagt gaattcccac
     4441 caggtgccca cagctcctgg acttcaaatt ctatatattg agagagttgg agagtatatc
     4501 agagatattt ttggaaagga gttggtctat gcaatgtcag tttggaatct tcttgaaagt
     4561 ttaatgtttt tattaggaga tttaaagaaa ataaaggtct acaatatctt taggtttttt
     4621 ttttttcctg tttaccgcac aaactgacca catggcatgt ctatcaggat ggagggtgtc
     4681 catgttctcc tctgtcttta gggaggtgat aaggagatgg gcggaggggt gtttttttct
     4741 ttgactcccc tcctttctaa cagaatgttg ccaccactgc ttgagtgggc tgtgtttgtt
     4801 cctctgtccc agcttctgtt gtagaaaata acattgttag gggaactcag gctagtgtca
     4861 gcgtcttggt ttggggagaa aaaattaaat gtttcggttt ttgtttcttt agtcgacctg
     4921 caggcatgca agctgatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
     4981 ccaccgctga gcaataacta gcataacccc ttggggcggc cgcttcgagc agacatgata
     5041 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     5101 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     5161 aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt
     5221 ttaagcaagt aaaacctcta caaatgtggt aaaatcgaat tttaacaaaa tattaacgct
     5281 tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     5341 cgcggatctg cgcagcacca tggcctgaaa taacctctga aagaggaact tggttaggta
     5401 ccttctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     5461 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     5521 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     5581 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     5641 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     5701 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     5761 aaaagcttga ttcttctgac acaacagtct cgaaccaaag gctggagcca ccatggcttc
     5821 caaggtgtac gaccccgagc aacgcaaacg catgatcact gggcctcagt ggtgggctcg
     5881 ctgcaagcaa atgaacgtgc tggactcctt catcaactac tatgattccg agaagcacgc
     5941 cgagaacgcc gtgatttttc tgcatggtaa cgctgcctcc agctacctgt ggaggcacgt
     6001 cgtgcctcac atcgagcccg tggctagatg catcatccct gatctgatcg gaatgggtaa
     6061 gtccggcaag agcgggaatg gctcatatcg cctcctggat cactacaagt acctcaccgc
     6121 ttggttcgag ctgctgaacc ttccaaagaa aatcatcttt gtgggccacg actggggggc
     6181 ttgtctggcc tttcactact cctacgagca ccaagacaag atcaaggcca tcgtccatgc
     6241 tgagagtgtc gtggacgtga tcgagtcctg ggacgagtgg cctgacatcg aggaggatat
     6301 cgccctgatc aagagcgaag agggcgagaa aatggtgctt gagaataact tcttcgtcga
     6361 gaccatgctc ccaagcaaga tcatgcggaa actggagcct gaggagttcg ctgcctacct
     6421 ggagccattc aaggagaagg gcgaggttag acggcctacc ctctcctggc ctcgcgagat
     6481 ccctctcgtt aagggaggca agcccgacgt cgtccagatt gtccgcaact acaacgccta
     6541 ccttcgggcc agcgacgatc tgcctaagat gttcatcgag tccgaccctg ggttcttttc
     6601 caacgctatt gtcgagggag ctaagaagtt ccctaacacc gagttcgtga aggtgaaggg
     6661 cctccacttc agccaggagg acgctccaga tgaaatgggt aagtacatca agagcttcgt
     6721 ggagcgcgtg ctgaagaacg agcagaccgg tggtgggagc ggaggtggcg gatcaggtgg
     6781 cggaggctcc ggagggattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     6841 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     6901 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     6961 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     7021 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     7081 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     7141 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     7201 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     7261 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     7321 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     7381 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     7441 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     7501 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     7561 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     7621 gcgacgccca acctgccatc acgatggccg caataaaata tctttatttt cattacatct
     7681 gtgtgttggt tttttgtgtg aatcgatagc gataaggatc ctctttgcgc ttgcgttttc
     7741 ccttgtccag atagcccagt agctgacatt catccggggt cagcaccgtt tctgcggact
     7801 ggctttctac gtaatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     7861 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     7921 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     7981 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     8041 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     8101 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     8161 gagcactttc aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     8221 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     8281 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     8341 gagtgataac actgcggcca acttacttct gacaactatc ggaggaccga aggagctaac
     8401 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     8461 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     8521 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     8581 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     8641 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     8701 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     8761 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     8821 attcgaaatg accgaccaag cgacgcccaa ccggtatcag ctcactcaaa ggcggtaata
     8881 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8941 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9001 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9061 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9121 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9181 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9241 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9301 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9361 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     9421 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9481 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9541 attacgcgca gaaaaaaagg atttcaagaa gatcctttga tcttttctac ggggtctgac
     9601 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9661 ttcacctaga tccttttata gtccggaaat acaggaacgc acgctggatg gcccttcgct
     9721 gggatggtga aaccatgaaa aatggcagct tcagtggatt aagtgggggt aatgtggcct
     9781 gtaccctctg gttgcatagg tattcatacg gttaaaattt atcaggcgcg attgcggcag
     9841 tttttcgggt ggtttgttgc catttttacc tgtctgctgc cgtgatcgcg ctgaacgcgt
     9901 tttagcggtg cgtacaatta agggattatg gtaaatccac ttactgt
//
