LOCUS       sequence-231492-        8805 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual luciferase assay for miRNA-targeted UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8805)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 8805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: pmiRGLO-PLEKHG5-212
FEATURES             Location/Qualifiers
     source          1..8805
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..523
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(59..82)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     456..475
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      564..573
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             570..2222
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    3845..3966
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3856..3875)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3910..3929
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        4211..4568
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4211..4402
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4234..4254)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4419..4554
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      4613..4622
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4619..5551
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             5603..6394
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5654..5673)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6264..6283
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6458..6506
                     /note="synthetic polyadenylation signal"
     promoter        6662..6766
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6767..6835,6836..7627)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6985..7004)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(7785..8373)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7865..7884)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    8489..8772
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 ggcgtagagg atcgagatct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
       61 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
      121 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
      181 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
      241 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
      301 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
      361 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
      421 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
      481 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt
      541 ggcaatccgg tactgttggt aaagccacca tggaagatgc caaaaacatt aagaagggcc
      601 cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac aaagccatga
      661 agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc gaggtggaca
      721 ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg aagcgctatg
      781 ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag ttcttcatgc
      841 ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac atctacaacg
      901 agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc gtgagcaaga
      961 aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa aagatcatca
     1021 tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc gtgacttccc
     1081 atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac cgggacaaaa
     1141 ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc gtagccctac
     1201 cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc ggcaaccaga
     1261 tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc ggcatgttca
     1321 ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc ttcgaggagg
     1381 agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg gtgcccacac
     1441 tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc aacttgcacg
     1501 agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg gccaaacgct
     1561 tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc gccattctga
     1621 tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc ttcttcgagg
     1681 ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc ggcgagctgt
     1741 gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct acaaacgctc
     1801 tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac gaggacgagc
     1861 acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac caggtagccc
     1921 cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc ggggtcgccg
     1981 gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg gaacacggta
     2041 aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca accgccaaga
     2101 agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc ggcaagttgg
     2161 acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag atcgccgtgt
     2221 aattctagtt gtttaaacga gctcggcaga gggaggcccc caagagtgcc attgaccaag
     2281 agacagcaga cagcctgcct cctggggcgt gccggcacct gcttcagcta ctgcctcctg
     2341 tatgcatgag ccggatgctg ggcaggatcc ctgcctacgc ccgggcccga tttgcgcttt
     2401 gccggactgg atggagtgga ggaggcccag gccacagtac caccccacct gcccaggcag
     2461 cccctcgtca cctactcccc gaagttacca gctcagctcg agtcttcagg gctgggctcc
     2521 taggctgccc atcctacttc taccctcact ggcctccagt gggattcact cctgccctgc
     2581 ccccaccttc ccagtcccac aggccacccc tggcttgggc tgggttctgt gaagttacgt
     2641 atttattgag cttttggttc ttttataaag acttgtctag actccactgg gaagagtcct
     2701 ttgctttggg gcccagtgac tcggggcact tgagttcagg gcggcctcct tgtgttcctg
     2761 tgctcctcca cttgccacgg atgggccacg gatggagctt gccatgggaa gcactgggaa
     2821 gtaatggggt gtggggtgcc accagaccaa cacccccaga cttccccacc ttcagccacc
     2881 atcagagcct ctccccaggt gccccccggg gattcagggc tgaatctgcc cagttcccac
     2941 actcaggcca gccctcttgg gaaggtgggt cctccatggg ggtcccttca ggaacttttt
     3001 tttttttttt aatacagagt ctcactctgt cacctaggtt ggagtgcagt ggtgtgatgt
     3061 cggctcactg caacctctgc ctcccgggtt caaacgattc tcctgcccca gccactctag
     3121 tagctggaac tgcaggtgtg caccaccacg ccgggctagt ttttgtattt taagtagaga
     3181 cggcatttca ccatattggt caggctggtc tcgaactcct gaccccaagt gttccgcccg
     3241 cctctgcctc ccatagtgct agaattacag gcctgagcta ctgcgcttgg ccccttgcgg
     3301 tacttttggc ccaacctcct ccatggctgg ggacgcggag gccgagagag aagtcacttg
     3361 ccctggctct accttgaagt ggttctcagg gttggggcga gagtcggggt ggggaccgag
     3421 atgcagctct atcctgtgcc cctggtcgca gcaggcagcc cagcgcttcg cgtgttctac
     3481 ttggcctgtc cgctgccgcc taatgagctc aggtctaggc cgagcagagg gggcacctgg
     3541 tcggactcgg ttgggctcgg gcggccccgc ctccccccgc ccgccaggcg ggcccttctc
     3601 gacggcgcgg ggcgggccct gcgggcgcgg ggctgaaggc ggaaccacga cgggcagaga
     3661 gcacggagcc gggaagcccc tgggcgcccg tcggagggct atggagcagc ggccgcagtc
     3721 gacctgcagg catgcaagct gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
     3781 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcggccgct tcgagcagac
     3841 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     3901 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     3961 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag
     4021 gtttttttaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgaatttta acaaaatatt
     4081 aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     4141 cgcatacgcg gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt
     4201 taggtacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     4261 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4321 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4381 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4441 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4501 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     4561 tttgcaaaaa gcttgattct tctgacacaa cagtctcgaa ccaaaggctg gagccaccat
     4621 ggcttccaag gtgtacgacc ccgagcaacg caaacgcatg atcactgggc ctcagtggtg
     4681 ggctcgctgc aagcaaatga acgtgctgga ctccttcatc aactactatg attccgagaa
     4741 gcacgccgag aacgccgtga tttttctgca tggtaacgct gcctccagct acctgtggag
     4801 gcacgtcgtg cctcacatcg agcccgtggc tagatgcatc atccctgatc tgatcggaat
     4861 gggtaagtcc ggcaagagcg ggaatggctc atatcgcctc ctggatcact acaagtacct
     4921 caccgcttgg ttcgagctgc tgaaccttcc aaagaaaatc atctttgtgg gccacgactg
     4981 gggggcttgt ctggcctttc actactccta cgagcaccaa gacaagatca aggccatcgt
     5041 ccatgctgag agtgtcgtgg acgtgatcga gtcctgggac gagtggcctg acatcgagga
     5101 ggatatcgcc ctgatcaaga gcgaagaggg cgagaaaatg gtgcttgaga ataacttctt
     5161 cgtcgagacc atgctcccaa gcaagatcat gcggaaactg gagcctgagg agttcgctgc
     5221 ctacctggag ccattcaagg agaagggcga ggttagacgg cctaccctct cctggcctcg
     5281 cgagatccct ctcgttaagg gaggcaagcc cgacgtcgtc cagattgtcc gcaactacaa
     5341 cgcctacctt cgggccagcg acgatctgcc taagatgttc atcgagtccg accctgggtt
     5401 cttttccaac gctattgtcg agggagctaa gaagttccct aacaccgagt tcgtgaaggt
     5461 gaagggcctc cacttcagcc aggaggacgc tccagatgaa atgggtaagt acatcaagag
     5521 cttcgtggag cgcgtgctga agaacgagca gaccggtggt gggagcggag gtggcggatc
     5581 aggtggcgga ggctccggag ggattgaaca agatggattg cacgcaggtt ctccggccgc
     5641 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     5701 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     5761 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     5821 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     5881 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     5941 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     6001 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     6061 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     6121 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     6181 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     6241 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     6301 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     6361 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     6421 gaccaagcga cgcccaacct gccatcacga tggccgcaat aaaatatctt tattttcatt
     6481 acatctgtgt gttggttttt tgtgtgaatc gatagcgata aggatcctct ttgcgcttgc
     6541 gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc accgtttctg
     6601 cggactggct ttctacgtaa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     6661 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6721 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6781 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6841 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6901 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     6961 aatgatgagc actttcaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7021 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7081 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7141 aaccatgagt gataacactg cggccaactt acttctgaca actatcggag gaccgaagga
     7201 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7261 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7321 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7381 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7441 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7501 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7561 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7621 ttggtaattc gaaatgaccg accaagcgac gcccaaccgg tatcagctca ctcaaaggcg
     7681 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7741 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7801 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7861 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7921 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7981 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     8041 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     8101 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     8161 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     8221 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     8281 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     8341 cagcagatta cgcgcagaaa aaaaggattt caagaagatc ctttgatctt ttctacgggg
     8401 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     8461 aggatcttca cctagatcct tttatagtcc ggaaatacag gaacgcacgc tggatggccc
     8521 ttcgctggga tggtgaaacc atgaaaaatg gcagcttcag tggattaagt gggggtaatg
     8581 tggcctgtac cctctggttg cataggtatt catacggtta aaatttatca ggcgcgattg
     8641 cggcagtttt tcgggtggtt tgttgccatt tttacctgtc tgctgccgtg atcgcgctga
     8701 acgcgtttta gcggtgcgta caattaaggg attatggtaa atccacttac tgtctgccct
     8761 cgtagccatc gagataaacc gcagtactcc ggccacgatg cgtcc
//
