LOCUS       sequence-231498-       10131 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual luciferase assay for miRNA-targeted UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10131)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 10131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: pmiRGLO-UBE2G2-202
FEATURES             Location/Qualifiers
     source          1..10131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..512
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(48..71)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     445..464
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      553..562
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             559..2211
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    5160..5281
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5171..5190)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5225..5244
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5526..5883
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5526..5717
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5549..5569)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5734..5869
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      5928..5937
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5934..6866
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             6918..7709
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6969..6988)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7579..7598
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7773..7821
                     /note="synthetic polyadenylation signal"
     promoter        7977..8081
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8082..8150,8151..8942)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8300..8319)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(9100..9688)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9180..9199)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    9804..10087
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcgagatcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
       61 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
      121 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
      181 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
      241 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
      301 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
      361 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
      421 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
      481 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagcttg gcaatccggt
      541 actgttggta aagccaccat ggaagatgcc aaaaacatta agaagggccc agcgccattc
      601 tacccactcg aagacgggac cgccggcgag cagctgcaca aagccatgaa gcgctacgcc
      661 ctggtgcccg gcaccatcgc ctttaccgac gcacatatcg aggtggacat tacctacgcc
      721 gagtacttcg agatgagcgt tcggctggca gaagctatga agcgctatgg gctgaataca
      781 aaccatcgga tcgtggtgtg cagcgagaat agcttgcagt tcttcatgcc cgtgttgggt
      841 gccctgttca tcggtgtggc tgtggcccca gctaacgaca tctacaacga gcgcgagctg
      901 ctgaacagca tgggcatcag ccagcccacc gtcgtattcg tgagcaagaa agggctgcaa
      961 aagatcctca acgtgcaaaa gaagctaccg atcatacaaa agatcatcat catggatagc
     1021 aagaccgact accagggctt ccaaagcatg tacaccttcg tgacttccca tttgccaccc
     1081 ggcttcaacg agtacgactt cgtgcccgag agcttcgacc gggacaaaac catcgccctg
     1141 atcatgaaca gtagtggcag taccggattg cccaagggcg tagccctacc gcaccgcacc
     1201 gcttgtgtcc gattcagtca tgcccgcgac cccatcttcg gcaaccagat catccccgac
     1261 accgctatcc tcagcgtggt gccatttcac cacggcttcg gcatgttcac cacgctgggc
     1321 tacttgatct gcggctttcg ggtcgtgctc atgtaccgct tcgaggagga gctattcttg
     1381 cgcagcttgc aagactataa gattcaatct gccctgctgg tgcccacact atttagcttc
     1441 ttcgctaaga gcactctcat cgacaagtac gacctaagca acttgcacga gatcgccagc
     1501 ggcggggcgc cgctcagcaa ggaggtaggt gaggccgtgg ccaaacgctt ccacctacca
     1561 ggcatccgcc agggctacgg cctgacagaa acaaccagcg ccattctgat cacccccgaa
     1621 ggggacgaca agcctggcgc agtaggcaag gtggtgccct tcttcgaggc taaggtggtg
     1681 gacttggaca ccggtaagac actgggtgtg aaccagcgcg gcgagctgtg cgtccgtggc
     1741 cccatgatca tgagcggcta cgttaacaac cccgaggcta caaacgctct catcgacaag
     1801 gacggctggc tgcacagcgg cgacatcgcc tactgggacg aggacgagca cttcttcatc
     1861 gtggaccggc tgaagagcct gatcaaatac aagggctacc aggtagcccc agccgaactg
     1921 gagagcatcc tgctgcaaca ccccaacatc ttcgacgccg gggtcgccgg cctgcccgac
     1981 gacgatgccg gcgagctgcc cgccgcagtc gtcgtgctgg aacacggtaa aaccatgacc
     2041 gagaaggaga tcgtggacta tgtggccagc caggttacaa ccgccaagaa gctgcgcggt
     2101 ggtgttgtgt tcgtggacga ggtgcctaaa ggactgaccg gcaagttgga cgcccgcaag
     2161 atccgcgaga ttctcattaa ggccaagaag ggcggcaaga tcgccgtgta attctagttg
     2221 tttaaacgag ctcggacctg gcctcgcaca ggcgcgcaca caccgccaag cagctcagca
     2281 ttctcccccg gcacacttag tgacagtgat gctctgtgct ggtaccaaac aaggcagact
     2341 tgcaagaacc atggcatctt tttttttttt caaacctttc ctacttcaaa caggcttctc
     2401 ttctgaaatg atgacttaat gtcgaatatt gacagcttac tgcagtttta cagtattcct
     2461 cacaaagggc ttcaggtaga ttatcagagc tgtcagcact acctctcccc gctgaaacca
     2521 gcagttcatg gcttcctgtg gattccctcc ctccctggag tgttgagggg gttgtacctg
     2581 ccagacttcc aggggacgat ggaataccca gaacgctcct tctgaagaaa tggggccctg
     2641 tagctgcagc acaggggaag ggcccggcac cctttctggg tccttcctgg ttccctgtgg
     2701 gccccatgag gagtccatta cttcctttct tccttcatat tttacaggca gatgcttttc
     2761 ttataatcta attacatctt ttcatttgtt atatattaca aaccatcaca cttagaaata
     2821 cttccaggaa atgctttttt gaagtgtgaa ttaataagaa atggggtaaa tagaaaagaa
     2881 atttattgct gattggccag gtgcggtggt tcgtgcctgt aatcccagct ctttgggagg
     2941 ccaaggcagg tagatcacaa ggtcaggaaa ttgagaccat cctggctaat acagtgaaac
     3001 cccatgtctg ctaaaattac aaaaaattag ctgggcgtgg tggtgcacgc ctgtagtctc
     3061 agctactcag gaggctgagg caggagaatc gcttgaaccc gggaggcaga ggtagcagtg
     3121 agctgaagtc ccgccactgc actccagcct gggcaacaga gcgagactca gtctcaaaaa
     3181 gaaaaaagaa atttattgct gatcacaagg acagacagtt ttttcccgac catactcatc
     3241 aaagatttac gtttgtatat tagtaactag tgcattacta gagcaggtgc aggtgaggtc
     3301 tttaaagttt caatgaaagt ttcttctgga tctacagaaa aaattttttt ttttcaatct
     3361 aaaaactgga aattctaggg tttttgtaca ttttggatgc actgggaatt tattagcaca
     3421 aaatcattct ttgcaactca aaattcagaa gggactctac catatcttag ctcagagcac
     3481 agaggagtgc cttatcccca cacttgactg ggctgtggag gtgggcatgt gggcccctgg
     3541 gcccaggctg gggacagagc ccttgttttg tgacttagga ttttgatgtg gttcccatgt
     3601 tctctaacag ggccagctga gcagcacagg ccaggaggcc acagtgtaag caataacaga
     3661 tctgccacat gcagaagcaa atatcaggcc tgtcgcacac gggcggcatt taaataggaa
     3721 tttctatttt tgaaataagg gatggtctat gaggcataca gtagatttga tgtgatcctt
     3781 ttctccctcc cttccataat ggatcgtggt ctgtgtgact gaacccacac agagtgtcat
     3841 gggtgacagt ttctggttga agtagctcca cgcctggctt ctgtggacag cagattcttt
     3901 tccttctcac aaggggctca tttaaaattt ggaggctggg tgctgtggct cacgcctgca
     3961 atcccagcac tttgggagac tgaggcgggc ggatcatgag gtcaggagat cgcgaccatc
     4021 ctggctaaca gtgaaaccct gtctccacta aaaatacaaa aaattagccg ggcgtggtgg
     4081 cgggcgcctg tagtcccagc tactctgaag actgaggcag gagaatggcg tgaacccagg
     4141 aggcggagct tgcagtgagc tgagatcacg ccactgcact ccagcctggg caacagagtg
     4201 agactctgtc tcaaaaaaaa aaaaaaaaaa aatggaacgc agggcaagaa ctcgtatttg
     4261 gaaggagatg ggggaaagga gcggtattat acctatgttg tatttgcagg caaatgagat
     4321 ggagccctct ctgtaaagaa gagtcatttg tgcaagtaga cggggtctgt gggtgcaggc
     4381 cctggagggg cacacaattg cctggaggct tctgtgagat cgggagaggg aagagaggca
     4441 gtctcttgac aaaataaagt atttttattc atttgtattt attaaatgaa aaaacaatcc
     4501 catggtgtcc ctgttgtgtg gtggaaccta atgactgttg aaataaagtt ctgtgttttc
     4561 cctgccctgc agtgtgtgtc cctttgtcgt attgtcagag gagcctgtgg ccactgcccc
     4621 cagggagcac accagatcat cccagctggg attcctcacg gtgggggctt ttctgcagac
     4681 ttgggttagc tctggcatgt tttcttctgt ttctgatctc ccttctgtca ttctgtggtc
     4741 ttcctctgga attctcaggt tagttggggt ctgtgagacg gatgtgttca ctaacctata
     4801 aatgacattt tgcagctttt tcagcagatg ccattgtgtg agtggcacat ttgaccacta
     4861 gaagccagca ggctgttccg actgcgcttc cctcctgcag ttgcttagaa gcacagctcc
     4921 ctgtcatcac tcctctgtgg tcctctgtgg tcacggttgg ttgcgatgag acctgcctct
     4981 gcgtcttgtt acttagcatg tcaataaaga ggcagattcc tgtgtcagtg aagtcgacct
     5041 gcaggcatgc aagctgatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
     5101 gccaccgctg agcaataact agcataaccc cttggggcgg ccgcttcgag cagacatgat
     5161 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     5221 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     5281 taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt
     5341 tttaagcaag taaaacctct acaaatgtgg taaaatcgaa ttttaacaaa atattaacgc
     5401 ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     5461 acgcggatct gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt
     5521 accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     5581 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     5641 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5701 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     5761 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     5821 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     5881 aaaaagcttg attcttctga cacaacagtc tcgaaccaaa ggctggagcc accatggctt
     5941 ccaaggtgta cgaccccgag caacgcaaac gcatgatcac tgggcctcag tggtgggctc
     6001 gctgcaagca aatgaacgtg ctggactcct tcatcaacta ctatgattcc gagaagcacg
     6061 ccgagaacgc cgtgattttt ctgcatggta acgctgcctc cagctacctg tggaggcacg
     6121 tcgtgcctca catcgagccc gtggctagat gcatcatccc tgatctgatc ggaatgggta
     6181 agtccggcaa gagcgggaat ggctcatatc gcctcctgga tcactacaag tacctcaccg
     6241 cttggttcga gctgctgaac cttccaaaga aaatcatctt tgtgggccac gactgggggg
     6301 cttgtctggc ctttcactac tcctacgagc accaagacaa gatcaaggcc atcgtccatg
     6361 ctgagagtgt cgtggacgtg atcgagtcct gggacgagtg gcctgacatc gaggaggata
     6421 tcgccctgat caagagcgaa gagggcgaga aaatggtgct tgagaataac ttcttcgtcg
     6481 agaccatgct cccaagcaag atcatgcgga aactggagcc tgaggagttc gctgcctacc
     6541 tggagccatt caaggagaag ggcgaggtta gacggcctac cctctcctgg cctcgcgaga
     6601 tccctctcgt taagggaggc aagcccgacg tcgtccagat tgtccgcaac tacaacgcct
     6661 accttcgggc cagcgacgat ctgcctaaga tgttcatcga gtccgaccct gggttctttt
     6721 ccaacgctat tgtcgaggga gctaagaagt tccctaacac cgagttcgtg aaggtgaagg
     6781 gcctccactt cagccaggag gacgctccag atgaaatggg taagtacatc aagagcttcg
     6841 tggagcgcgt gctgaagaac gagcagaccg gtggtgggag cggaggtggc ggatcaggtg
     6901 gcggaggctc cggagggatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     6961 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     7021 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     7081 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     7141 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     7201 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     7261 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     7321 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     7381 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     7441 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     7501 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     7561 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     7621 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     7681 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     7741 agcgacgccc aacctgccat cacgatggcc gcaataaaat atctttattt tcattacatc
     7801 tgtgtgttgg ttttttgtgt gaatcgatag cgataaggat cctctttgcg cttgcgtttt
     7861 cccttgtcca gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac
     7921 tggctttcta cgtaatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     7981 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     8041 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     8101 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     8161 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     8221 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     8281 tgagcacttt caaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     8341 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     8401 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     8461 tgagtgataa cactgcggcc aacttacttc tgacaactat cggaggaccg aaggagctaa
     8521 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     8581 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     8641 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     8701 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     8761 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     8821 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     8881 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     8941 aattcgaaat gaccgaccaa gcgacgccca accggtatca gctcactcaa aggcggtaat
     9001 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     9061 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     9121 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     9181 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     9241 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     9301 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     9361 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9421 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9481 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9541 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9601 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9661 gattacgcgc agaaaaaaag gatttcaaga agatcctttg atcttttcta cggggtctga
     9721 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9781 cttcacctag atccttttat agtccggaaa tacaggaacg cacgctggat ggcccttcgc
     9841 tgggatggtg aaaccatgaa aaatggcagc ttcagtggat taagtggggg taatgtggcc
     9901 tgtaccctct ggttgcatag gtattcatac ggttaaaatt tatcaggcgc gattgcggca
     9961 gtttttcggg tggtttgttg ccatttttac ctgtctgctg ccgtgatcgc gctgaacgcg
    10021 ttttagcggt gcgtacaatt aagggattat ggtaaatcca cttactgtct gccctcgtag
    10081 ccatcgagat aaaccgcagt actccggcca cgatgcgtcc ggcgtagagg a
//
