LOCUS       sequence-23150-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   He TC, Sparks AB, Rago C, Hermeking H, Zawel L, da Costa LT, Morin 
            PJ, Vogelstein B, Kinzler KW
  TITLE     Identification of c-MYC as a target of the APC pathway.
  JOURNAL   Science. 1998 Sep 4. 281(5382):1509-12.
  PUBMED    9727977
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1998 Sep 4. 281(5382):1509-12."
COMMENT     Sequence Label: c-myc promoter (4x TBE1 - mut)
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      50..59
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    269..360
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(301..320)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    374..422
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(690..711)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcnttcgcgg anganggcgc cgggcctttc tttatgtttt tggcgtcttc catggtggct
       61 ttaccaacag taccggaatg ccaagcttac ttagatcgca gatctcgata ccggatccat
      121 tatatacccg aattcgatat cgacgtccag ctgtcgagcc cgggctagca gaccggtttc
      181 ctgcagaccg gtttcctgcg ctagcagacc ggtttcctgc agaccggttt cctgcgctag
      241 cacgcgtaag agctcggtac ctatcgatag agaaatgttc tggcacctgc acttgcactg
      301 gggacagcct attttgctag tttgttttgt ttcgttttgt tttgatggag agcgtatgtt
      361 agtactatcg attcacacaa aaaaccaaca cacagatgta atgaaaataa agatatttta
      421 ttgcggccgc tccaagtacc tcccgtacct taatattact tacttatcat ggtagcttgg
      481 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
      541 atggcgaatg gcaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
      601 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
      661 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
      721 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca ggaggnggcc cnctacgtga
      781 acatcnccct aatcaagttt ttggggtcga ggtgccgtaa gcactaatcg
//
