LOCUS       sequence-231521-       10730 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for NLS-iRFP-NLS, JNK KTR-mCherry, and mKO-MK2 in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10730)
  AUTHORS   Miura H, Kondo Y, Matsuda M, Aoki K
  TITLE     Cell-to-Cell Heterogeneity in p38-Mediated Cross-Inhibition of JNK 
            Causes Stochastic Cell Death.
  JOURNAL   Cell Rep. 2018 Sep 4;24(10):2658-2668. doi: 
            10.1016/j.celrep.2018.08.020.
  PUBMED    30184500
REFERENCE   2  (bases 1 to 10730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.08"; journalName: "Cell Rep"; date: 
            "2018-09-4- 4"; volume: "24"; issue: "10"; pages: "2658-2668"
COMMENT     Sequence Label: pNJP
FEATURES             Location/Qualifiers
     source          1..10730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..657
                     /label=chicken beta-actin promoter
     intron          658..1674
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1598..1620
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1682..1701
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1771..1791
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2773..2793
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2803..2859
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3049..3753
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHFTRGMDELY"
     primer_bind     complement(3194..3212)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3462..3481
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3763..3819
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3820..4473
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELGEDAVAHS"
     misc_feature    5784..6333
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5928..5945)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6155..6174
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(6804..6821)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6810..7034
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7081..7097)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7105..7121
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7129..7135,7136..7153,7154..7159))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7174..7195
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   7429..7491
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7532..7552)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7532..7550)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7571..7587)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7595..7611
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7619..7625,7626..7643,7644..7649))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7664..7685
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7802..7819)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7973..8561)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8053..8072)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8732..9523,9524..9592))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9355..9374
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9593..9697)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9723..10178)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9860..9881)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10072..10091
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10305..10327
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10319..10336
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10320..10336
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10346..10365
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10346..10364
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   10394..10428
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc
      541 ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
      601 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
      661 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
      721 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
      781 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
      841 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
      901 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      961 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     1081 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattggg atccgaattt
     1741 catcattttg gcaaagaatt caccatggcc cctaagaaga agcgtaaggt gatggccgaa
     1801 gggagcgtgg caagacagcc cgatctgctg acatgcgacg acgaaccaat ccacattcca
     1861 ggagctatcc agcctcatgg actgctgctg gctctggccg ctgacatgac tatcgtggca
     1921 ggctctgata acctgccaga gctgaccggg ctggctatcg gagcactgat tggccggtcc
     1981 gcagccgacg tgttcgattc tgagactcac aatagactga ccatcgccct ggctgaacct
     2041 ggggctgcag tgggagcccc aattaccgtc ggcttcacaa tgcgaaagga cgctggcttt
     2101 atcgggtcct ggcaccgcca tgatcagctg atttttctgg agctggaacc acctcagaga
     2161 gacgtggcag agccacaggc tttctttcgg agaacaaaca gcgccatcag gcgcctgcag
     2221 gccgctgaga ctctggaatc cgcttgcgca gccgctgcac aggaagtgag gaagatcacc
     2281 gggttcgatc gcgtcatgat ctacagattc gcctcagact ttagcggaga agtgatcgca
     2341 gaagatcgct gtgccgaggt cgaaagtaaa ctgggcctgc attacccagc ctcaaccgtg
     2401 cctgcacagg cacgacggct gtatacaatc aaccccgtgc ggatcattcc cgacattaat
     2461 tacagaccag tgcccgtcac acctgacctg aacccagtga ctgggaggcc catcgatctg
     2521 agcttcgcca ttctgcgcag cgtgtcccct gtccacctgg agtttatgag gaatatcgga
     2581 atgcatggca ccatgtctat cagtattctg agaggcgaaa ggctgtgggg gctgatcgtg
     2641 tgccaccatc ggacacctta ctatgtggac ctggacggca ggcaggcttg tgaactggtg
     2701 gctcaggtgc tggcttggca gattggcgtg atgaaggagg ggcatggcac cggcagcacc
     2761 ggcagcggca gccctaagaa gaagcgtaag gtggggagcg gggctaccaa cttctccctc
     2821 ctcaagcagg caggggacgt cgaggaaaat cctggaccca tgagtaaccc taagatccta
     2881 aaacagagca tgaccttgaa cctggccgac ccggtgggca gtctgaagcc gcacctccgc
     2941 gccaagcgct cggccggtct caacgaagac gagtcgccat ctaagaagct ggcgtcgccg
     3001 gaggtgtcgt caaggctgga gcgcctgacc ctccagtcca gcggccgcat ggtgagcaag
     3061 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     3121 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     3181 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     3241 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     3301 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     3361 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     3421 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     3481 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     3541 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     3601 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     3661 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     3721 cgccacttca ctcgcggcat ggacgagctg tacgggtcag gggccaccaa cttctctctt
     3781 ctgaaacaag ccggcgacgt cgaggaaaac cccggaccta tggtgagcgt gatcaagccc
     3841 gagatgaaga tgaggtacta catggacggc tccgtcaatg ggcatgagtt cacaatcgag
     3901 ggtgagggca caggcagacc ttacgaggga catcaggaga tgacactgcg cgtcacaatg
     3961 gccaagggcg ggccaatgcc tttcgccttc gacctggtgt cccacgtgtt ctgttacggc
     4021 cacagacctt ttactaaata tccagaagag atcccagact atttcaagca ggcctttcct
     4081 gagggcctgt cctgggagag gtccctggag ttcgaggacg gcggctccgc ctccgtgagc
     4141 gcccacatca gcctgagggg caacaccttc taccacaagt ccaagttcac cggcgtgaac
     4201 ttccccgccg acggccccat catgcagaac cagagcgtgg actgggagcc ctccaccgag
     4261 aagatcaccg ccagcgacgg cgtgctgaag ggcgacgtga ccatgtacct gaagctggag
     4321 ggcggcggca accacaagtg ccagttcaag accacctaca aggccgccaa gaagatcctg
     4381 aagatgcccg gcagccacta catcagccac aggctggtga ggaagaccga gggcaacatc
     4441 accgagctgg gggaggacgc cgtggcccac tccctcgaga tgctgtccaa ctcccagggc
     4501 cagagcccgc cggtgccgtt ccccgccccg gccccgccgc cgcagccccc cacccctgcc
     4561 ctgccgcacc ccccggcgca gccgccgccg ccgcccccgc agcagttccc gcagttccac
     4621 gtcaagtccg gcctgcagat caagaagaac gccatcatcg atgactacaa ggtcaccagc
     4681 caggtcctgg ggctgggcat caacggcaaa gttttgcaga tcttcaacaa gaggacccag
     4741 gagaaattcg ccctcaaaat gcttcaggac tgccccaagg cccgcaggga ggtggagctg
     4801 cactggcggg cctcccagtg cccgcacatc gtacggatcg tggatgtgta cgagaatctg
     4861 tacgcaggga ggaagtgcct gctgattgtc atggaatgtt tggacggtgg agaactcttt
     4921 agccgaatcc aggatcgagg agaccaggca ttcacagaaa gagaagcatc cgaaatcatg
     4981 aagagcatcg gtgaggccat ccagtatctg cattcaatca acattgccca tcgggatgtc
     5041 aagcctgaga atctcttata cacctccaaa aggcccaacg ccatcctgaa actcactgac
     5101 tttggctttg ccaaggaaac caccagccac aactctttga ccactccttg ttatacaccg
     5161 tactatgtgg ctccagaagt gctgggtcca gagaagtatg acaagtcctg tgacatgtgg
     5221 tccctgggtg tcatcatgta catcctgctg tgtgggtatc cccccttcta ctccaaccac
     5281 ggccttgcca tctctccggg catgaagact cgcatccgaa tgggccagta tgaatttccc
     5341 aacccagaat ggtcagaagt atcagaggaa gtgaagatgc tcattcggaa tctgctgaaa
     5401 acagagccca cccagagaat gaccatcacc gagtttatga accacccttg gatcatgcaa
     5461 tcaacaaagg tccctcaaac cccactgcac accagccggg tcctgaagga ggacaaggag
     5521 cggtgggagg atgtcaagga ggagatgacc agtgccttgg ccacaatgcg cgttgactac
     5581 gagcagatca agataaaaaa gattgaagat gcatccaacc ctctgctgct gaagaggcgg
     5641 aagaaagctc gggccctgga ggctgcggct ctggcccact gaggcggccg ctctagagtc
     5701 gacgggccgc ggtaacaatt gttaactaac ttaagctagc aacggtttcc ctctagcggg
     5761 atcaattccg cccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     5821 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     5881 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     5941 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     6001 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     6061 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     6121 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     6181 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     6241 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     6301 ggacgtggtt ttcctttgaa aaacacgata ataccatggt catgaaaaca tttaacattt
     6361 ctcaacaaga tctagaatta gtagaagtag cgacagagaa gattacaatg ctttatgagg
     6421 ataataaaca tcatgtggga gcggcaattc gtacgaaaac aggagaaatc atttcggcag
     6481 tacatattga agcgtatata ggacgagtaa ctgtttgtgc agaagccatt gcgattggta
     6541 gtgcagtttc gaatggacaa aaggattttg acacgattgt agctgttaga cacccttatt
     6601 ctgacgaagt agatagaagt attcgagtgg taagtccttg tggtatgtgt agggagttga
     6661 tttcagacta tgcaccagat tgttttgtgt taatagaaat gaatggcaag ttagtcaaaa
     6721 ctacgattga agaactcatt ccactcaaat atacccgaaa ttaaaagtcg agatgcatcg
     6781 atgatctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     6841 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     6901 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     6961 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     7021 ggtgggctct atggcttctg aggcggaaag aacctgcagc ccaagcttgg cgtaatcatg
     7081 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7141 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7201 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tcttaaaagt
     7261 tttgttactt tatagaagaa attttgagtt tttgtttttt tttaataaat aaataaacat
     7321 aaataaattg tttgttgaat ttattattag tatgtaagtg taaatataat aaaacttaat
     7381 atctattcaa attaataaat aaacctcgat atacagaccg ataaaacaca tgcgtcaatt
     7441 ttacgcatga ttatctttaa cgtacgtcac aatatgatta tctttctagg gttaatctag
     7501 tatacgcgtt taattaatcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg
     7561 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     7621 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     7681 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     7741 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     7801 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7861 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7921 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7981 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8041 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8101 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8161 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8221 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8281 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     8341 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8401 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     8461 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     8521 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     8581 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     8641 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8701 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8761 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8821 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8881 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8941 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9001 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     9061 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9121 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9181 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9241 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9301 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     9361 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9421 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     9481 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     9541 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     9601 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9661 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     9721 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     9781 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     9841 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     9901 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     9961 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
    10021 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
    10081 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
    10141 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
    10201 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
    10261 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
    10321 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
    10381 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
    10441 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
    10501 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
    10561 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
    10621 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
    10681 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc
//
