LOCUS       sequence-231522-       10237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual luciferase assay for miRNA-targeted UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10237)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 10237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: pmiRGLO-TMEM134-201
FEATURES             Location/Qualifiers
     source          1..10237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..520
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(56..79)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     453..472
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      561..570
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             567..2219
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    5274..5395
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5285..5304)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5339..5358
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5640..5997
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5640..5831
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5663..5683)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5848..5983
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      6042..6051
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6048..6980
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             7032..7823
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7083..7102)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7693..7712
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7887..7935
                     /note="synthetic polyadenylation signal"
     promoter        8091..8195
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8196..8264,8265..9056)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8414..8433)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(9214..9802)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9294..9313)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    9918..10201
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 gtagaggatc gagatctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
       61 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
      121 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
      181 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
      241 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
      301 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
      361 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
      421 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
      481 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttggc
      541 aatccggtac tgttggtaaa gccaccatgg aagatgccaa aaacattaag aagggcccag
      601 cgccattcta cccactcgaa gacgggaccg ccggcgagca gctgcacaaa gccatgaagc
      661 gctacgccct ggtgcccggc accatcgcct ttaccgacgc acatatcgag gtggacatta
      721 cctacgccga gtacttcgag atgagcgttc ggctggcaga agctatgaag cgctatgggc
      781 tgaatacaaa ccatcggatc gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg
      841 tgttgggtgc cctgttcatc ggtgtggctg tggccccagc taacgacatc tacaacgagc
      901 gcgagctgct gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag
      961 ggctgcaaaa gatcctcaac gtgcaaaaga agctaccgat catacaaaag atcatcatca
     1021 tggatagcaa gaccgactac cagggcttcc aaagcatgta caccttcgtg acttcccatt
     1081 tgccacccgg cttcaacgag tacgacttcg tgcccgagag cttcgaccgg gacaaaacca
     1141 tcgccctgat catgaacagt agtggcagta ccggattgcc caagggcgta gccctaccgc
     1201 accgcaccgc ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc aaccagatca
     1261 tccccgacac cgctatcctc agcgtggtgc catttcacca cggcttcggc atgttcacca
     1321 cgctgggcta cttgatctgc ggctttcggg tcgtgctcat gtaccgcttc gaggaggagc
     1381 tattcttgcg cagcttgcaa gactataaga ttcaatctgc cctgctggtg cccacactat
     1441 ttagcttctt cgctaagagc actctcatcg acaagtacga cctaagcaac ttgcacgaga
     1501 tcgccagcgg cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc
     1561 acctaccagg catccgccag ggctacggcc tgacagaaac aaccagcgcc attctgatca
     1621 cccccgaagg ggacgacaag cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta
     1681 aggtggtgga cttggacacc ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg
     1741 tccgtggccc catgatcatg agcggctacg ttaacaaccc cgaggctaca aacgctctca
     1801 tcgacaagga cggctggctg cacagcggcg acatcgccta ctgggacgag gacgagcact
     1861 tcttcatcgt ggaccggctg aagagcctga tcaaatacaa gggctaccag gtagccccag
     1921 ccgaactgga gagcatcctg ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc
     1981 tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa
     2041 ccatgaccga gaaggagatc gtggactatg tggccagcca ggttacaacc gccaagaagc
     2101 tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg actgaccggc aagttggacg
     2161 cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc gccgtgtaat
     2221 tctagttgtt taaacgagct cgtcgcggcg cagcgtggac cccttgcgcc catgggggcg
     2281 cccctcttgc cctgttccgt tcccctcatc tcaagggaag aggccctcca ggaccctcga
     2341 aaccccagcc cctagggagt ttgctcagga agttcggggc atgcaggcct ggccctggga
     2401 aagccgcccg tcgcctgctc tgtgccttaa cttattctcg ggccgtgcgg ctgctaggtt
     2461 gctgttattt tgtgctaata aaagagtaat taattccacc agaggcaatt gctctcctcc
     2521 tgtcctgcca ctgctagccc agcccaccag cctgaccaca gcccccctcc caaggactga
     2581 ccctgacgct gaagggcttc gagcaccacc gaacgcgtgg cagccctgat gctgaggctt
     2641 gcccaagggc acggggcatg ttaggggcag agctgggcgt aggactcctg ccttgccagc
     2701 caggagccgg ccggaacagc ctgccccacc cccagccctt ggatgactca accagcccgt
     2761 ctctgtctgg tggccctggc cgagggcacg cagagatgac gcaggcctgg gacctggagg
     2821 ctggtatcct agttccagcc tggctctggg gccctgggct gccacagcca gccctgaatg
     2881 tgggccatgt caaggcaatg gcccagcccc ctccacccac tgccctcctt cccagtccca
     2941 gaggttggga gaacctgggg gcagagggca taggaagccg cgcctgacag acaccctcag
     3001 tggtcaaaga cttagaaatg cagaacgtta gaaacaaacc atctcagaat tttggaacat
     3061 cagcatttca gaatcttacg aaacaagaaa cagaatcttt gagaaacctg aaatctgaga
     3121 tcatagtagc taccagttat caatttgttg aaagacccca ggatggccgg gcacggtggc
     3181 tcaagcctgt aatcctagca ctttgagagg ccaaggtggg aggatcgctt gagcccagga
     3241 gttcgcgacc aacctggaca acatggcgag aacccatctc tatttaaaaa aaaaaaaaga
     3301 aaagaaaata tactaggccc tgcgctaagc actgaacacg ggttatatct tgtaatcttc
     3361 actacctccg tcccatttta cagataagga ctgactcacc caaggacagc cagcaggtgg
     3421 taggtagaat taggaacgta gcccagggct cttcccatga cactgtcggg gagggagaag
     3481 gaaaccggca ctgagctggt ctcccctacc ggccaaccaa gaggctggct gagtgagcaa
     3541 gggttgggag gccctgttgg tgcacctgcc cgtactgtct atctccccca ccaaaacacg
     3601 ggtcccacaa accactgtct gcagcaccca acacagcggg gccttcagga ttctgagtta
     3661 gaggaatgga cacccagact cttgctctcg ctcccacctt tccaggcccc gcagacttcc
     3721 caccctcatc ccccattcaa ggcacttagg gggcacccat tggatgcagg aactcctaaa
     3781 ccagtggtcc cccaactagc tatggtaaag gaccagtttt catttctaat ccatcacggt
     3841 ccagtacttt cataagaaga actagccggg tgccgtgcct catgcctgta atcccagcac
     3901 tttgagaggc cacggtggga ggatcacttg agcccaggag ttcgagacca acctgggaaa
     3961 catgggaaaa ccccatctct acaaaaaata caaaaactta gccaggcatg gtggggcgcc
     4021 tgtagtccca cccacttgcg aggctggagt gggaggatca cggagcctgg gaggctgcag
     4081 tggagtcatg attgcaccac tgcactccag cctggaagac agactgagac ctatttaaaa
     4141 aataaataaa taaataacat aaaaatttta aaaactagaa aaatgaaata aaaaacatac
     4201 agaggctggg cgtggtggct catgcctgca atcccagcac tttgggaggc cgaggcaggt
     4261 gaatcatgag gtcaggagtt cgagaccagc ctggccaaca gggtgaaatc ccatttctat
     4321 taaaaataca aaaaaattac cgggcatggt ggtgcgcacc tgtaatccca gctactcagg
     4381 aggctgaggc aggagaatcg cttgaacccg ggaggcagag gttgcagtga gccgagatca
     4441 cgccactaca ctccagcctg agcgacagag cgagactctg tctccaaaaa aaaaaaaata
     4501 cagaatacaa gtccacagtt tatcagatta attagacata aaattacatt tcaataaata
     4561 taatcagaac aaacatagga aaaaggaaaa atgacaaaaa ccattgaata tgaacaaaca
     4621 ggcaattaac acagaatcgc agctaacaac tttttactcc ttgttttttt tttttttgtt
     4681 ttttttttga gacgagtctc actgtctccc aggctggagt gcagtggccc gatctcggct
     4741 cactgcatcc tctgcctccc ggtttaagcg attctcatgc ctcagccttc tcagtagctg
     4801 cgactacagg cacatgccac catgcccagc taatctttgt atttttagta gagacagggt
     4861 ttcactatgt tggccaggct ggtctccaac ccctgagcca cctcgcccga cttcattcta
     4921 cagtcttaac agaaagcaat ttcagaagtg gtgattccca tcattagaag tctgcgttag
     4981 gaatcagcaa ggtatagtcc acagagtggt ttttatattt tgtaatggtt ggagaaaagt
     5041 caaagaataa taagatttta tgacataaaa ttatataaaa ttcaaatttc catgtcaata
     5101 aatgaagttt tactgttatt tattaaaatt tctgatatag ccacaagtcg acctgcaggc
     5161 atgcaagctg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
     5221 gctgagcaat aactagcata accccttggg gcggccgctt cgagcagaca tgataagata
     5281 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     5341 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     5401 caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg tttttttaag
     5461 caagtaaaac ctctacaaat gtggtaaaat cgaattttaa caaaatatta acgcttacaa
     5521 tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgcgg
     5581 atctgcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt aggtaccttc
     5641 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     5701 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     5761 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5821 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5881 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     5941 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     6001 cttgattctt ctgacacaac agtctcgaac caaaggctgg agccaccatg gcttccaagg
     6061 tgtacgaccc cgagcaacgc aaacgcatga tcactgggcc tcagtggtgg gctcgctgca
     6121 agcaaatgaa cgtgctggac tccttcatca actactatga ttccgagaag cacgccgaga
     6181 acgccgtgat ttttctgcat ggtaacgctg cctccagcta cctgtggagg cacgtcgtgc
     6241 ctcacatcga gcccgtggct agatgcatca tccctgatct gatcggaatg ggtaagtccg
     6301 gcaagagcgg gaatggctca tatcgcctcc tggatcacta caagtacctc accgcttggt
     6361 tcgagctgct gaaccttcca aagaaaatca tctttgtggg ccacgactgg ggggcttgtc
     6421 tggcctttca ctactcctac gagcaccaag acaagatcaa ggccatcgtc catgctgaga
     6481 gtgtcgtgga cgtgatcgag tcctgggacg agtggcctga catcgaggag gatatcgccc
     6541 tgatcaagag cgaagagggc gagaaaatgg tgcttgagaa taacttcttc gtcgagacca
     6601 tgctcccaag caagatcatg cggaaactgg agcctgagga gttcgctgcc tacctggagc
     6661 cattcaagga gaagggcgag gttagacggc ctaccctctc ctggcctcgc gagatccctc
     6721 tcgttaaggg aggcaagccc gacgtcgtcc agattgtccg caactacaac gcctaccttc
     6781 gggccagcga cgatctgcct aagatgttca tcgagtccga ccctgggttc ttttccaacg
     6841 ctattgtcga gggagctaag aagttcccta acaccgagtt cgtgaaggtg aagggcctcc
     6901 acttcagcca ggaggacgct ccagatgaaa tgggtaagta catcaagagc ttcgtggagc
     6961 gcgtgctgaa gaacgagcag accggtggtg ggagcggagg tggcggatca ggtggcggag
     7021 gctccggagg gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     7081 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     7141 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     7201 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     7261 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     7321 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     7381 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     7441 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     7501 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     7561 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     7621 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     7681 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     7741 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     7801 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     7861 gcccaacctg ccatcacgat ggccgcaata aaatatcttt attttcatta catctgtgtg
     7921 ttggtttttt gtgtgaatcg atagcgataa ggatcctctt tgcgcttgcg ttttcccttg
     7981 tccagatagc ccagtagctg acattcatcc ggggtcagca ccgtttctgc ggactggctt
     8041 tctacgtaat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     8101 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     8161 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     8221 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     8281 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     8341 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     8401 ctttcaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     8461 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     8521 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     8581 ataacactgc ggccaactta cttctgacaa ctatcggagg accgaaggag ctaaccgctt
     8641 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     8701 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     8761 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     8821 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     8881 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     8941 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     9001 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaattcg
     9061 aaatgaccga ccaagcgacg cccaaccggt atcagctcac tcaaaggcgg taatacggtt
     9121 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     9181 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     9241 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     9301 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     9361 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     9421 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     9481 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     9541 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     9601 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     9661 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     9721 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     9781 gcgcagaaaa aaaggatttc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     9841 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     9901 ctagatcctt ttatagtccg gaaatacagg aacgcacgct ggatggccct tcgctgggat
     9961 ggtgaaacca tgaaaaatgg cagcttcagt ggattaagtg ggggtaatgt ggcctgtacc
    10021 ctctggttgc ataggtattc atacggttaa aatttatcag gcgcgattgc ggcagttttt
    10081 cgggtggttt gttgccattt ttacctgtct gctgccgtga tcgcgctgaa cgcgttttag
    10141 cggtgcgtac aattaaggga ttatggtaaa tccacttact gtctgccctc gtagccatcg
    10201 agataaaccg cagtactccg gccacgatgc gtccggc
//
