LOCUS       sequence-231523-        9984 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual luciferase assay for miRNA-targeted UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9984)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 9984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: pmiRGLO-SPRED2-201
FEATURES             Location/Qualifiers
     source          1..9984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     276..295
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      384..393
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             390..2042
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    4844..4965
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4855..4874)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4909..4928
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5210..5567
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5210..5401
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5233..5253)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5418..5553
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      5612..5621
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5618..6550
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             6602..7393
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6653..6672)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7263..7282
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7457..7505
                     /note="synthetic polyadenylation signal"
     promoter        7661..7765
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7766..7834,7835..8626)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7984..8003)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(8784..9372)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8864..8883)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    9488..9771
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     promoter        join(9828..9984,1..343)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9863..9886)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
       61 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
      121 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
      181 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
      241 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
      301 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt
      361 ggcaatccgg tactgttggt aaagccacca tggaagatgc caaaaacatt aagaagggcc
      421 cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac aaagccatga
      481 agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc gaggtggaca
      541 ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg aagcgctatg
      601 ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag ttcttcatgc
      661 ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac atctacaacg
      721 agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc gtgagcaaga
      781 aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa aagatcatca
      841 tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc gtgacttccc
      901 atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac cgggacaaaa
      961 ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc gtagccctac
     1021 cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc ggcaaccaga
     1081 tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc ggcatgttca
     1141 ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc ttcgaggagg
     1201 agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg gtgcccacac
     1261 tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc aacttgcacg
     1321 agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg gccaaacgct
     1381 tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc gccattctga
     1441 tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc ttcttcgagg
     1501 ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc ggcgagctgt
     1561 gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct acaaacgctc
     1621 tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac gaggacgagc
     1681 acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac caggtagccc
     1741 cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc ggggtcgccg
     1801 gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg gaacacggta
     1861 aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca accgccaaga
     1921 agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc ggcaagttgg
     1981 acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag atcgccgtgt
     2041 aattctagtt gtttaaacga gctcgctcag tttccctccc ttctccctcc atccgcagcc
     2101 acaggggaac tcgtctctta catactctca tcttctcccc cgctcccttc cactccaagg
     2161 agcgaggagg gcaagcggcc tcccagctcc ctggtacctc gaggcaccat tccagccagg
     2221 gacgctgccg ggtagactct ccactccccc tgccgcccac actgcagcag ccacatccat
     2281 acacacacgc tcgcacagtg ttctgaggaa ggaaccttcg ccacagactc ctgtactatt
     2341 aacaatctgt aaccaagcta actgtctcat ccatgtgttg atttcctgtt tcctcctccc
     2401 ccgcctcttc cagttcaaag gagtctgcaa ttggaactgc tgattttcgg tgggttttgt
     2461 agttgatttt tccaagagcg tcgaagactc tctttctctt ggttcacctt gcctgtcgct
     2521 agcaagcatc tggttcagcg gaaatgggat gtgagaatga tgaaacccga cagaagtatc
     2581 tcagcctgca gtcagttatt atgtatagga ggtgagctag ttaacaaact tgtaccacaa
     2641 aacaatatcg ctttaacttt tctaaagcca aatttcccat gtaagctgca gtttctatct
     2701 ttagcccatc atcattttct gcccccccaa aatctgttga aatgattcac tgatgcaaaa
     2761 cattcacccg taatagactg agaattgagc cttaacttca gattaacttg tgagaatcag
     2821 gaaaattcct tcaactgcat tgcatcctct tggaccaggg ctagaatggg gatttcaggt
     2881 ttctatgagc ctccccatta cccctaaagt agaacatttt ttaaattgtg ttgccaccac
     2941 ttctaaaaat ttagcaatat tagaatagga tactagtgaa gtaagaaatt ttgcttgttg
     3001 ttttgcaaac caaatagttt cctcaaacac aaatcagtgt tcccacgaac aagttccagt
     3061 tgagaaacac taaggttatg gtgaataaaa ccatagggag cttcttcccc ccaacccctg
     3121 gctattttat attaatgggg agaggggatt tttaaatgtc ataaatttga agagtggtgg
     3181 gttgcatttt cttcatgggt ttatgttttc gtttcatttt ggactcaatt tcacatcacc
     3241 aaattcctca tttatacttg gggaaaaaac aaggccatat gtaaaaaccc ttccaatgcc
     3301 taagtgtctt tctcctgcaa ctccaaaccc agactcgcca ctttgggtgc acaggtggtt
     3361 aggtcagcca actggttctg cctgtcgcct tgccacggag gaggcttcta attagctgga
     3421 aaagagtatt tttctaatac gttgcaagga ttagccaaat cttcttattg aagaaagaag
     3481 aaaagtgaag agtggttacc tatacctagc atagtacaat cagaacctcg tggagaccac
     3541 cggggacagg cttgcggacg ccggctgttc ttcccgccac gatctttctg tggtagcggc
     3601 cagcagaaga catggcctgc tccccactcc ctttccccac tccttcttta ttgcacacag
     3661 gacaccagtc ttcaaggaaa gggacttttt tccagtctgc caatcatatt gggaaagtgc
     3721 tagctgtgct caccttcatg gggctgtttc cagctcgtcc acaagctcat cgattttctt
     3781 tagtagatta ccggtgtaaa tacccagtgt gcttatgagt cagttagtag acgtcttcat
     3841 tcattggagt aactggttta ggctttccag tttggaaaag gagcagagag ctgtccatct
     3901 ggattgatgg aagaaaagag aacctcatcc atgcctggag aacatctaga aaaccttcag
     3961 ccagcctcca gtgctgtcga gagaccacct tcccccgacc cggaggcact tccttggggt
     4021 ctttctctag ggtctcttct ctacaaagca caacactaat gttcgtttcc ttagacctca
     4081 gttcaagtgc ccctatttat ttcaataaga acgcacatat cccagctgtt ttttgtttgt
     4141 cacctctatt tagttgttac ctgtttctct cttctttcac cccttgtcct tttccaccct
     4201 tttaagagtt acgctagcag atcttactcc acgtatactt tttggtttgt gaaggcatcg
     4261 gttaagggca caaagacagc catggggaca tttatgtaaa tacgtctcta attgccacac
     4321 tgcagctgaa cagtgtgtag tattttccca gtcagctttg ccatactgac gtcaatcatt
     4381 tgagagaaat tattcagatt ttatttttgt atctgtggta acaaaacatt aaccaaaaga
     4441 ttttctgtcc agaagcctcc ccgacccccc aagctatttg ctcacattaa caaattaaag
     4501 tgcctgaagc ataattcatt ctttacctgt atactaaaaa ccctgttgta ttgatttttt
     4561 tataataagc ctttttacct ctgtgtaaaa aatatatata caagtgtatg atgtacattt
     4621 tagttcttaa ctttttttta tggtttctaa tatgtatgac caatgtagcc attgctttaa
     4681 aatgtaccgt gtaaatataa acacatccta tcagaagtcg acctgcaggc atgcaagctg
     4741 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
     4801 aactagcata accccttggg gcggccgctt cgagcagaca tgataagata cattgatgag
     4861 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     4921 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     4981 attcatttta tgtttcaggt tcagggggag atgtgggagg tttttttaag caagtaaaac
     5041 ctctacaaat gtggtaaaat cgaattttaa caaaatatta acgcttacaa tttcctgatg
     5101 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgcgg atctgcgcag
     5161 caccatggcc tgaaataacc tctgaaagag gaacttggtt aggtaccttc tgaggcggaa
     5221 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     5281 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     5341 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     5401 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     5461 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     5521 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttgattctt
     5581 ctgacacaac agtctcgaac caaaggctgg agccaccatg gcttccaagg tgtacgaccc
     5641 cgagcaacgc aaacgcatga tcactgggcc tcagtggtgg gctcgctgca agcaaatgaa
     5701 cgtgctggac tccttcatca actactatga ttccgagaag cacgccgaga acgccgtgat
     5761 ttttctgcat ggtaacgctg cctccagcta cctgtggagg cacgtcgtgc ctcacatcga
     5821 gcccgtggct agatgcatca tccctgatct gatcggaatg ggtaagtccg gcaagagcgg
     5881 gaatggctca tatcgcctcc tggatcacta caagtacctc accgcttggt tcgagctgct
     5941 gaaccttcca aagaaaatca tctttgtggg ccacgactgg ggggcttgtc tggcctttca
     6001 ctactcctac gagcaccaag acaagatcaa ggccatcgtc catgctgaga gtgtcgtgga
     6061 cgtgatcgag tcctgggacg agtggcctga catcgaggag gatatcgccc tgatcaagag
     6121 cgaagagggc gagaaaatgg tgcttgagaa taacttcttc gtcgagacca tgctcccaag
     6181 caagatcatg cggaaactgg agcctgagga gttcgctgcc tacctggagc cattcaagga
     6241 gaagggcgag gttagacggc ctaccctctc ctggcctcgc gagatccctc tcgttaaggg
     6301 aggcaagccc gacgtcgtcc agattgtccg caactacaac gcctaccttc gggccagcga
     6361 cgatctgcct aagatgttca tcgagtccga ccctgggttc ttttccaacg ctattgtcga
     6421 gggagctaag aagttcccta acaccgagtt cgtgaaggtg aagggcctcc acttcagcca
     6481 ggaggacgct ccagatgaaa tgggtaagta catcaagagc ttcgtggagc gcgtgctgaa
     6541 gaacgagcag accggtggtg ggagcggagg tggcggatca ggtggcggag gctccggagg
     6601 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     6661 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     6721 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     6781 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     6841 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     6901 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     6961 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     7021 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     7081 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     7141 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     7201 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     7261 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     7321 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     7381 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     7441 ccatcacgat ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt
     7501 gtgtgaatcg atagcgataa ggatcctctt tgcgcttgcg ttttcccttg tccagatagc
     7561 ccagtagctg acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacgtaat
     7621 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     7681 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     7741 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     7801 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     7861 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     7921 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca ctttcaaagt
     7981 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     8041 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     8101 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     8161 ggccaactta cttctgacaa ctatcggagg accgaaggag ctaaccgctt ttttgcacaa
     8221 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     8281 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     8341 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     8401 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     8461 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     8521 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     8581 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaattcg aaatgaccga
     8641 ccaagcgacg cccaaccggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     8701 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     8761 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     8821 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     8881 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     8941 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     9001 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     9061 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     9121 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     9181 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     9241 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     9301 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     9361 aaaggatttc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     9421 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     9481 ttatagtccg gaaatacagg aacgcacgct ggatggccct tcgctgggat ggtgaaacca
     9541 tgaaaaatgg cagcttcagt ggattaagtg ggggtaatgt ggcctgtacc ctctggttgc
     9601 ataggtattc atacggttaa aatttatcag gcgcgattgc ggcagttttt cgggtggttt
     9661 gttgccattt ttacctgtct gctgccgtga tcgcgctgaa cgcgttttag cggtgcgtac
     9721 aattaaggga ttatggtaaa tccacttact gtctgccctc gtagccatcg agataaaccg
     9781 cagtactccg gccacgatgc gtccggcgta gaggatcgag atctaccggg taggggaggc
     9841 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     9901 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     9961 gttctttggt ggccccttcg cgcc
//
