LOCUS       sequence-231524-        9389 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual luciferase assay for miRNA-targeted UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9389)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 9389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: pmiRGLO-PDK2-203
FEATURES             Location/Qualifiers
     source          1..9389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        38..537
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(73..96)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     470..489
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      578..587
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             584..2236
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    4443..4564
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4454..4473)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4508..4527
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        4809..5166
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4809..5000
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4832..4852)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5017..5152
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      5211..5220
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5217..6149
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             6201..6992
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6252..6271)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6862..6881
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7056..7104
                     /note="synthetic polyadenylation signal"
     promoter        7260..7364
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7365..7433,7434..8225)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7583..7602)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(8383..8971)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8463..8482)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    9087..9370
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 gccacgatgc gtccggcgta gaggatcgag atctaccggg taggggaggc gcttttccca
       61 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
      121 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt
      181 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag
      241 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg
      301 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt
      361 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg
      421 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
      481 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
      541 tgcagcccaa gcttggcaat ccggtactgt tggtaaagcc accatggaag atgccaaaaa
      601 cattaagaag ggcccagcgc cattctaccc actcgaagac gggaccgccg gcgagcagct
      661 gcacaaagcc atgaagcgct acgccctggt gcccggcacc atcgccttta ccgacgcaca
      721 tatcgaggtg gacattacct acgccgagta cttcgagatg agcgttcggc tggcagaagc
      781 tatgaagcgc tatgggctga atacaaacca tcggatcgtg gtgtgcagcg agaatagctt
      841 gcagttcttc atgcccgtgt tgggtgccct gttcatcggt gtggctgtgg ccccagctaa
      901 cgacatctac aacgagcgcg agctgctgaa cagcatgggc atcagccagc ccaccgtcgt
      961 attcgtgagc aagaaagggc tgcaaaagat cctcaacgtg caaaagaagc taccgatcat
     1021 acaaaagatc atcatcatgg atagcaagac cgactaccag ggcttccaaa gcatgtacac
     1081 cttcgtgact tcccatttgc cacccggctt caacgagtac gacttcgtgc ccgagagctt
     1141 cgaccgggac aaaaccatcg ccctgatcat gaacagtagt ggcagtaccg gattgcccaa
     1201 gggcgtagcc ctaccgcacc gcaccgcttg tgtccgattc agtcatgccc gcgaccccat
     1261 cttcggcaac cagatcatcc ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg
     1321 cttcggcatg ttcaccacgc tgggctactt gatctgcggc tttcgggtcg tgctcatgta
     1381 ccgcttcgag gaggagctat tcttgcgcag cttgcaagac tataagattc aatctgccct
     1441 gctggtgccc acactattta gcttcttcgc taagagcact ctcatcgaca agtacgacct
     1501 aagcaacttg cacgagatcg ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc
     1561 cgtggccaaa cgcttccacc taccaggcat ccgccagggc tacggcctga cagaaacaac
     1621 cagcgccatt ctgatcaccc ccgaagggga cgacaagcct ggcgcagtag gcaaggtggt
     1681 gcccttcttc gaggctaagg tggtggactt ggacaccggt aagacactgg gtgtgaacca
     1741 gcgcggcgag ctgtgcgtcc gtggccccat gatcatgagc ggctacgtta acaaccccga
     1801 ggctacaaac gctctcatcg acaaggacgg ctggctgcac agcggcgaca tcgcctactg
     1861 ggacgaggac gagcacttct tcatcgtgga ccggctgaag agcctgatca aatacaaggg
     1921 ctaccaggta gccccagccg aactggagag catcctgctg caacacccca acatcttcga
     1981 cgccggggtc gccggcctgc ccgacgacga tgccggcgag ctgcccgccg cagtcgtcgt
     2041 gctggaacac ggtaaaacca tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt
     2101 tacaaccgcc aagaagctgc gcggtggtgt tgtgttcgtg gacgaggtgc ctaaaggact
     2161 gaccggcaag ttggacgccc gcaagatccg cgagattctc attaaggcca agaagggcgg
     2221 caagatcgcc gtgtaattct agttgtttaa acgagctcgg ggccgccgtg catctgcacc
     2281 tgagaggacg gactgccgcc tctgggtccc cccaccgtgg tgcccctcac catcctcctg
     2341 ggggagcagg gggtgggttc tccctgatga ccaggttctg tctctatgga agtcactgcg
     2401 gtgataggtc tgtgatggtc cctaagtgcc agtccatctc tgtggagacc cctcggtggc
     2461 ctccctgtct ctgtgggcga tgcctgaggg ttagggatgt ctccaccctg atggggtgtc
     2521 ccagagacat tttcccatgg cagtcctcct ctctgagacc agggctgtca cttttctgcc
     2581 aggggtactg ggtccccctc agcaccctcc acagcacagg ccttccaagt ggatgtcccg
     2641 ttgccttatt cccccagccc acaaaggcac cctggccttg gcctgctgaa gtgttaggaa
     2701 gagggtgggt gccctccaga cctggggact gagtggggaa aggagttaca cccgtgagtg
     2761 gggaatgagg ctggtcctgc agcctctccc tccgctcagg gcttgaaggt cggtggcgga
     2821 gggggtggct ctcacagggc ccaactctaa agtggaagaa ccttgttaga ccgagagctt
     2881 gccatccagc caagctgctc gaggccctgc agtggccttg gcaatgtctg tgccacctcc
     2941 tgagccctcc cagcatgtcc tcacatgctc atgcccaccc gctcctccac aagcctagtc
     3001 catcctgcct gagctccagc ccccagcccc cactgtgccc agacatgtgt gctcagggtg
     3061 gctttctccc taggaccttc tgtgtatata gttagtttta taaccctgaa tgcccccacc
     3121 cttcccctaa gcacacaggg gttaaagctg tgtgtccctc ccagtggctg tggcagtgac
     3181 agtgacaccc acacccacag taaagaggag actgaatgag actggcctgg ctgcatccct
     3241 gggggagggg acccacacgt gcgcacgtac acacgcacac tgcggtgccc tgtgaccacc
     3301 acatgacgca ccggggacgt ccggcccagc tcaggactgt gagtaaaaca ctgggcaaag
     3361 ccatctgttc agaacgccgg atggcccagc acctggtgac agacctgttg tcccaccata
     3421 ctcagggccc agcagagtcc ggccaaccca acaactggag cagacagaaa ttctggtcac
     3481 tggcacatca taaggattta ttgaaataaa ttgagaactg cctccccttc acatgcaggt
     3541 gggcacccac tatagctact gatggcttta aggatgtggg tgccttccaa cctcccatgg
     3601 ccctgccacc aggcccagat ggtgccagtg tcctcatatc tgtttccctc cgaagcccct
     3661 ctcccagcac agatagcaga ggggggataa gggctgagtc ccaggtacag ggtgctggcc
     3721 gtaagccaca gcccctgggc aggaatgagg gagccagcat gcattggtgc catctgtcgc
     3781 tctgtctggg cagatgggag gagctggagg ggtttccaag cgggagcccc caggcctcat
     3841 cggtaactct gaccagtgtc ctggaagcgg gactagctgc tgaccagcgc cacacatgaa
     3901 cacttcctcc aaggacattt cctaaaaagg acacatgctg tccccaggct gctggtaaac
     3961 tccaccccca tccagggagc tggccccagc cctcagtgtg gcccagggaa gcagagcttg
     4021 caccgcctgc cagttctgag cccactgatt ccctgacaat gcctggggca gatgggggag
     4081 ggctgatgaa cctttccaat ctcgtagctg ccggggaccc ccaggttttg cctctctgcc
     4141 tggctggggt gagggctgac caggtcagca gctcctaggc cttggctggt ggaagtggtg
     4201 tcctctgtcc ctatcagttc ctgtttgctc agctcccaaa gaggacttag gtgccctctg
     4261 aggaagcagc aggaagtgaa gggagtcctg gcttggagaa tgggtcaaag accaagtcga
     4321 cctgcaggca tgcaagctga tccggctgct aacaaagccc gaaaggaagc tgagttggct
     4381 gctgccaccg ctgagcaata actagcataa ccccttgggg cggccgcttc gagcagacat
     4441 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     4501 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     4561 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt
     4621 ttttttaagc aagtaaaacc tctacaaatg tggtaaaatc gaattttaac aaaatattaa
     4681 cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     4741 catacgcgga tctgcgcagc accatggcct gaaataacct ctgaaagagg aacttggtta
     4801 ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
     4861 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4921 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4981 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     5041 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     5101 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     5161 tgcaaaaagc ttgattcttc tgacacaaca gtctcgaacc aaaggctgga gccaccatgg
     5221 cttccaaggt gtacgacccc gagcaacgca aacgcatgat cactgggcct cagtggtggg
     5281 ctcgctgcaa gcaaatgaac gtgctggact ccttcatcaa ctactatgat tccgagaagc
     5341 acgccgagaa cgccgtgatt tttctgcatg gtaacgctgc ctccagctac ctgtggaggc
     5401 acgtcgtgcc tcacatcgag cccgtggcta gatgcatcat ccctgatctg atcggaatgg
     5461 gtaagtccgg caagagcggg aatggctcat atcgcctcct ggatcactac aagtacctca
     5521 ccgcttggtt cgagctgctg aaccttccaa agaaaatcat ctttgtgggc cacgactggg
     5581 gggcttgtct ggcctttcac tactcctacg agcaccaaga caagatcaag gccatcgtcc
     5641 atgctgagag tgtcgtggac gtgatcgagt cctgggacga gtggcctgac atcgaggagg
     5701 atatcgccct gatcaagagc gaagagggcg agaaaatggt gcttgagaat aacttcttcg
     5761 tcgagaccat gctcccaagc aagatcatgc ggaaactgga gcctgaggag ttcgctgcct
     5821 acctggagcc attcaaggag aagggcgagg ttagacggcc taccctctcc tggcctcgcg
     5881 agatccctct cgttaaggga ggcaagcccg acgtcgtcca gattgtccgc aactacaacg
     5941 cctaccttcg ggccagcgac gatctgccta agatgttcat cgagtccgac cctgggttct
     6001 tttccaacgc tattgtcgag ggagctaaga agttccctaa caccgagttc gtgaaggtga
     6061 agggcctcca cttcagccag gaggacgctc cagatgaaat gggtaagtac atcaagagct
     6121 tcgtggagcg cgtgctgaag aacgagcaga ccggtggtgg gagcggaggt ggcggatcag
     6181 gtggcggagg ctccggaggg attgaacaag atggattgca cgcaggttct ccggccgctt
     6241 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     6301 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     6361 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     6421 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     6481 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     6541 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     6601 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     6661 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     6721 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     6781 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     6841 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     6901 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     6961 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     7021 ccaagcgacg cccaacctgc catcacgatg gccgcaataa aatatcttta ttttcattac
     7081 atctgtgtgt tggttttttg tgtgaatcga tagcgataag gatcctcttt gcgcttgcgt
     7141 tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac cgtttctgcg
     7201 gactggcttt ctacgtaatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     7261 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     7321 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     7381 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     7441 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     7501 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     7561 tgatgagcac tttcaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     7621 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     7681 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     7741 ccatgagtga taacactgcg gccaacttac ttctgacaac tatcggagga ccgaaggagc
     7801 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     7861 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     7921 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     7981 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     8041 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     8101 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     8161 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     8221 ggtaattcga aatgaccgac caagcgacgc ccaaccggta tcagctcact caaaggcggt
     8281 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8341 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8401 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8461 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8521 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8581 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8641 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8701 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8761 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8821 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8881 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8941 gcagattacg cgcagaaaaa aaggatttca agaagatcct ttgatctttt ctacggggtc
     9001 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9061 gatcttcacc tagatccttt tatagtccgg aaatacagga acgcacgctg gatggccctt
     9121 cgctgggatg gtgaaaccat gaaaaatggc agcttcagtg gattaagtgg gggtaatgtg
     9181 gcctgtaccc tctggttgca taggtattca tacggttaaa atttatcagg cgcgattgcg
     9241 gcagtttttc gggtggtttg ttgccatttt tacctgtctg ctgccgtgat cgcgctgaac
     9301 gcgttttagc ggtgcgtaca attaagggat tatggtaaat ccacttactg tctgccctcg
     9361 tagccatcga gataaaccgc agtactccg
//
