LOCUS       sequence-231529-        9559 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual luciferase assay for miRNA-targeted UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9559)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 9559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: pmiRGLO-UBE2I-202
FEATURES             Location/Qualifiers
     source          1..9559
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        51..550
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(86..109)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     483..502
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      591..600
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             597..2249
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    4626..4747
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4637..4656)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4691..4710
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        4992..5349
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4992..5183
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5015..5035)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5200..5335
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      5394..5403
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5400..6332
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             6384..7175
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6435..6454)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7045..7064
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7239..7287
                     /note="synthetic polyadenylation signal"
     promoter        7443..7547
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7548..7616,7617..8408)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7766..7785)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(8566..9154)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8646..8665)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    9270..9553
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 ccgcagtact ccggccacga tgcgtccggc gtagaggatc gagatctacc gggtagggga
       61 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
      121 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
      181 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
      241 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
      301 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
      361 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg
      421 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
      481 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
      541 gggcctttcg acctgcagcc caagcttggc aatccggtac tgttggtaaa gccaccatgg
      601 aagatgccaa aaacattaag aagggcccag cgccattcta cccactcgaa gacgggaccg
      661 ccggcgagca gctgcacaaa gccatgaagc gctacgccct ggtgcccggc accatcgcct
      721 ttaccgacgc acatatcgag gtggacatta cctacgccga gtacttcgag atgagcgttc
      781 ggctggcaga agctatgaag cgctatgggc tgaatacaaa ccatcggatc gtggtgtgca
      841 gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc cctgttcatc ggtgtggctg
      901 tggccccagc taacgacatc tacaacgagc gcgagctgct gaacagcatg ggcatcagcc
      961 agcccaccgt cgtattcgtg agcaagaaag ggctgcaaaa gatcctcaac gtgcaaaaga
     1021 agctaccgat catacaaaag atcatcatca tggatagcaa gaccgactac cagggcttcc
     1081 aaagcatgta caccttcgtg acttcccatt tgccacccgg cttcaacgag tacgacttcg
     1141 tgcccgagag cttcgaccgg gacaaaacca tcgccctgat catgaacagt agtggcagta
     1201 ccggattgcc caagggcgta gccctaccgc accgcaccgc ttgtgtccga ttcagtcatg
     1261 cccgcgaccc catcttcggc aaccagatca tccccgacac cgctatcctc agcgtggtgc
     1321 catttcacca cggcttcggc atgttcacca cgctgggcta cttgatctgc ggctttcggg
     1381 tcgtgctcat gtaccgcttc gaggaggagc tattcttgcg cagcttgcaa gactataaga
     1441 ttcaatctgc cctgctggtg cccacactat ttagcttctt cgctaagagc actctcatcg
     1501 acaagtacga cctaagcaac ttgcacgaga tcgccagcgg cggggcgccg ctcagcaagg
     1561 aggtaggtga ggccgtggcc aaacgcttcc acctaccagg catccgccag ggctacggcc
     1621 tgacagaaac aaccagcgcc attctgatca cccccgaagg ggacgacaag cctggcgcag
     1681 taggcaaggt ggtgcccttc ttcgaggcta aggtggtgga cttggacacc ggtaagacac
     1741 tgggtgtgaa ccagcgcggc gagctgtgcg tccgtggccc catgatcatg agcggctacg
     1801 ttaacaaccc cgaggctaca aacgctctca tcgacaagga cggctggctg cacagcggcg
     1861 acatcgccta ctgggacgag gacgagcact tcttcatcgt ggaccggctg aagagcctga
     1921 tcaaatacaa gggctaccag gtagccccag ccgaactgga gagcatcctg ctgcaacacc
     1981 ccaacatctt cgacgccggg gtcgccggcc tgcccgacga cgatgccggc gagctgcccg
     2041 ccgcagtcgt cgtgctggaa cacggtaaaa ccatgaccga gaaggagatc gtggactatg
     2101 tggccagcca ggttacaacc gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg
     2161 tgcctaaagg actgaccggc aagttggacg cccgcaagat ccgcgagatt ctcattaagg
     2221 ccaagaaggg cggcaagatc gccgtgtaat tctagttgtt taaacgagct cggcagcgac
     2281 cttgtggcat cgtcagaagg aagggattgg tttggcaaga acttgtttac aacatttttg
     2341 caaatctaaa gttgctccat acaatgacta gtcacctggg ggggttgggc gggcgccatc
     2401 ttccattgcc gccgcgggtg tgcggtctcg attcgctgaa ttgcccgttt ccatacaggg
     2461 tctcttcctt cggtcttttg tatttttgat tgttatgtaa aactcgcttt tattttaata
     2521 ttgatgtcag tatttcaact gctgtaaaat tataaacttt tatacttggg taagtccccc
     2581 aggggcgagt tcctcgctct gggatgcagg catgcttctc accgtgcaga gctgcacttg
     2641 gcctcagctg gctgtatgga aatgcaccct ccctcctgcc gctcctctct agaaccttct
     2701 agaacctggg ctgtgctgct tttgagcctc agaccccagg gcagcatctc ggttctgcgc
     2761 cacttccttt gtgtttatat ggcgttttgt ctgtgttgct gtttagagta aataaactgt
     2821 ttatataaag gttttggttg cattattatc attgaaagtg agaggaggcg gcctcccagt
     2881 gcccggccct ccccacccac ctgcagcccc accgcgggcc aggaccaggc tctccatctg
     2941 cttcggatgc acgcaggctg tgaggctctg tcttgccctg gatctttgta aacagggctg
     3001 tgtacaaagt gctgctgagg tttctgtgct ccccgcatct tcgggctgta gagcgctggg
     3061 cagctaagat ctgcataggt cgggattggc atcgagaccc tggcaactgc accggtgcca
     3121 gctgtcttgg gggccacaag gccaggtcca gaccagggct gggggctgcc tgaggactcc
     3181 tatccgggca gcctgctggc gggggttccc ctcttcagtg gccaggtcac agggatggag
     3241 ctgcgctgtg catagggtgc cacctcaggt gtctgtccct tgtgtcctca ggaggcagcc
     3301 ttgctaccac ccgtggcaaa cgccaggtgc tttttctggg agagcccaca gccgtggccc
     3361 tccagggctt ccccgaccct tagcgccagg tggagggccc tgggcagcct gtgtatggaa
     3421 ttcttcgtcc tgaggccgcc tgagtgtggt ctgtcctggg gaggctgtgc gcctcagcag
     3481 ccgtcctgac gctgagccct ctgcaaaggt tgggccggcc aggcctcttg gggctgcctg
     3541 agccactgca ggaagtggcc tggctgggaa gttgggtgcc ggtcacctcc cagcaggaag
     3601 gcacagtgga cagagatggg aagccctggg ggacacagcc cggtgctccc agccctccaa
     3661 cctctggctc ccaacccagt ctccccatcc tagcgagctt ggccctcctc agtttcgttt
     3721 caagccttgg ggctggagct ggccctgctg ccctggcacc ccccggtggc tggagctggg
     3781 tccccgtggc ccaagtgcag ggtcccaaga gggcagggcg gggctcccca aaggagcaaa
     3841 gaatgcaggg agggcggtcc aggcccctgg gaaggggagc tcggcaccct ccaggtccgt
     3901 gtgggacccc agccgctgtt ggctgggaat cgaagttaga ggtgacttcc aaaggccccc
     3961 cgagccggca gtgcccccca ccaccccccc agcgactctg cggtgccagt gccttgttgg
     4021 cttttccggc tacgcaccct gcagtcactg agctctcggt ctgacgtctg atgtttgtgg
     4081 tttgtttata acacggggcc ttacctgggg aattcagctg gtttgaatat ttgtagcccg
     4141 ctcccagaat gtcttatttt gtaatgactg aactacattt agtaatagtt acacatgtat
     4201 atggttaata catatggaaa ttcaatatat tttgtagtta acgtattctg aagtaacgga
     4261 tgtttctcgc caatcgtagt gacttcagct aacgaaatgt tcttttgtag taccacggtc
     4321 ctcggcctaa cgaaggacgt gaaccttgta agaggagagc tctgaaacgc ggtcaccttt
     4381 gtttagtgga agggaaagtg tgttcccggc atgaggtgcc tcggaattag taaagaattg
     4441 tgggcaatgg attaaccact gtatctaaga atccaccatt aaagcatttg cacagacagt
     4501 cgacctgcag gcatgcaagc tgatccggct gctaacaaag cccgaaagga agctgagttg
     4561 gctgctgcca ccgctgagca ataactagca taaccccttg gggcggccgc ttcgagcaga
     4621 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     4681 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     4741 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga
     4801 ggttttttta agcaagtaaa acctctacaa atgtggtaaa atcgaatttt aacaaaatat
     4861 taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     4921 ccgcatacgc ggatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg
     4981 ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     5041 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5101 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5161 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     5221 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     5281 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     5341 ttttgcaaaa agcttgattc ttctgacaca acagtctcga accaaaggct ggagccacca
     5401 tggcttccaa ggtgtacgac cccgagcaac gcaaacgcat gatcactggg cctcagtggt
     5461 gggctcgctg caagcaaatg aacgtgctgg actccttcat caactactat gattccgaga
     5521 agcacgccga gaacgccgtg atttttctgc atggtaacgc tgcctccagc tacctgtgga
     5581 ggcacgtcgt gcctcacatc gagcccgtgg ctagatgcat catccctgat ctgatcggaa
     5641 tgggtaagtc cggcaagagc gggaatggct catatcgcct cctggatcac tacaagtacc
     5701 tcaccgcttg gttcgagctg ctgaaccttc caaagaaaat catctttgtg ggccacgact
     5761 ggggggcttg tctggccttt cactactcct acgagcacca agacaagatc aaggccatcg
     5821 tccatgctga gagtgtcgtg gacgtgatcg agtcctggga cgagtggcct gacatcgagg
     5881 aggatatcgc cctgatcaag agcgaagagg gcgagaaaat ggtgcttgag aataacttct
     5941 tcgtcgagac catgctccca agcaagatca tgcggaaact ggagcctgag gagttcgctg
     6001 cctacctgga gccattcaag gagaagggcg aggttagacg gcctaccctc tcctggcctc
     6061 gcgagatccc tctcgttaag ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca
     6121 acgcctacct tcgggccagc gacgatctgc ctaagatgtt catcgagtcc gaccctgggt
     6181 tcttttccaa cgctattgtc gagggagcta agaagttccc taacaccgag ttcgtgaagg
     6241 tgaagggcct ccacttcagc caggaggacg ctccagatga aatgggtaag tacatcaaga
     6301 gcttcgtgga gcgcgtgctg aagaacgagc agaccggtgg tgggagcgga ggtggcggat
     6361 caggtggcgg aggctccgga gggattgaac aagatggatt gcacgcaggt tctccggccg
     6421 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     6481 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     6541 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     6601 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     6661 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     6721 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     6781 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     6841 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     6901 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     6961 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     7021 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     7081 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     7141 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     7201 cgaccaagcg acgcccaacc tgccatcacg atggccgcaa taaaatatct ttattttcat
     7261 tacatctgtg tgttggtttt ttgtgtgaat cgatagcgat aaggatcctc tttgcgcttg
     7321 cgttttccct tgtccagata gcccagtagc tgacattcat ccggggtcag caccgtttct
     7381 gcggactggc tttctacgta atggtttctt agacgtcagg tggcactttt cggggaaatg
     7441 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     7501 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     7561 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     7621 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     7681 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     7741 caatgatgag cactttcaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     7801 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     7861 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     7921 taaccatgag tgataacact gcggccaact tacttctgac aactatcgga ggaccgaagg
     7981 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     8041 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     8101 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     8161 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     8221 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     8281 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     8341 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     8401 attggtaatt cgaaatgacc gaccaagcga cgcccaaccg gtatcagctc actcaaaggc
     8461 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8521 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8581 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8641 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8701 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8761 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8821 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8881 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8941 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     9001 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9061 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     9121 gcagcagatt acgcgcagaa aaaaaggatt tcaagaagat cctttgatct tttctacggg
     9181 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     9241 aaggatcttc acctagatcc ttttatagtc cggaaataca ggaacgcacg ctggatggcc
     9301 cttcgctggg atggtgaaac catgaaaaat ggcagcttca gtggattaag tgggggtaat
     9361 gtggcctgta ccctctggtt gcataggtat tcatacggtt aaaatttatc aggcgcgatt
     9421 gcggcagttt ttcgggtggt ttgttgccat ttttacctgt ctgctgccgt gatcgcgctg
     9481 aacgcgtttt agcggtgcgt acaattaagg gattatggta aatccactta ctgtctgccc
     9541 tcgtagccat cgagataaa
//
