LOCUS       sequence-231541-       17030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac transposon for dox-inducible expression of the OKMS (Oct4, 
            Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette 
            (with mCherry). KRAB-dCas9 is constitutively expressed..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17030)
  AUTHORS   Kagawa H, Shimamoto R, Kim SI, Oceguera-Yanez F, Yamamoto T, 
            Schroeder T, Woltjen K
  TITLE     OVOL1 Influences the Determination and Expansion of iPSC 
            Reprogramming Intermediates.
  JOURNAL   Stem Cell Reports. 2018 Dec 28. pii: S2213-6711(18)30527-7. doi: 
            10.1016/j.stemcr.2018.12.008.
  PUBMED    30639212
REFERENCE   2  (bases 1 to 17030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2018 Dec 28. pii: S2213-6711(18)30527-7. doi: 
            10.1016/j.stemcr.2018.12.008."
COMMENT     Sequence Label: PB-TAC-OKMS-KRAB-dCas9
FEATURES             Location/Qualifiers
     source          1..17030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   711..773
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1145..1163
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1182..1200
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1219..1237
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1256..1274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1293..1311
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1330..1348
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1367..1385
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1404..1422
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1444..1468
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1548..1572
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2725..2790
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             4231..4290
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             5686..5751
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     protein_bind    complement(6791..6815)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(6857..7430,7431..7433,7434..7442)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7024..7041)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7251..7270
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7443..8153
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7588..7606)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7856..7875
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(8165..8184)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8230..8285
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8284..8303)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     enhancer        8720..9005
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        9007..9284
                     /label=chicken beta-actin promoter
     intron          9285..9513
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      9525..9534
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9561..9746
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     CDS             join(9768..9794,9795..9797,9798..12284,12285..12287,
                     12288..13871)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9768..13871
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             13875..13901
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             13881..13907
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             13920..13940
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             13947..13967
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(14031..14048)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    14037..14244
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   14655..14689
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(14839..14857)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(14878..14894)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14878..14894)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14891..14913)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14902..14918
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14926..14932,14933..14950,14951..14956))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14971..14992
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15109..15126)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15280..15868)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15360..15379)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16039..16830,16831..16899))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16662..16681
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16900..17004)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcgg
      661 tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa ccctagaaag
      721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta
      781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt
      841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa
      901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct
      961 tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt
     1021 aattagctta acctatacat aatagaaaca aattatactt attagtcagt cagaaacaac
     1081 tttggcacat atcaatatta tgctctcgtt aattaactcg agggatccaa gctggccgca
     1141 cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca gtgatagaga
     1201 aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct
     1261 atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac
     1321 gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat
     1381 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtaccc cctatataag
     1441 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     1501 ccatagaaga caccgggacc gatccagcct ccgcggtggc ggccatcaca agtttgtaca
     1561 aaaaagcagg ctggcgccgg aaccaattca gtcgactgga tccggtaccg aattcgccct
     1621 taaaaagcag gctccacctt ccccatggct ggacacctgg cttcagactt cgccttctca
     1681 cccccaccag gtgggggtga tgggtcagca gggctggagc cgggctgggt ggattctcga
     1741 acctggctaa gcttccaagg gcctccaggt gggcctggaa tcggaccagg ctcagaggta
     1801 ttggggatct ccccatgtcc gcccgcatac gagttctgcg gagggatggc atactgtgga
     1861 cctcaggttg gactgggcct agtcccccaa gttggcgtgg agactttgca gcctgagggc
     1921 caggcaggag cacgagtgga aagcaactca gagggaacct cctctgagcc ctgtgccgac
     1981 cgccccaatg ccgtgaagtt ggagaaggtg gaaccaactc ccgaggagtc ccaggacatg
     2041 aaagccctgc agaaggagct agaacagttt gccaagctgc tgaagcagaa gaggatcacc
     2101 ttggggtaca cccaggccga cgtggggctc accctgggcg ttctctttgg aaaggtgttc
     2161 agccagacca ccatctgtcg cttcgaggcc ttgcagctca gccttaagaa catgtgtaag
     2221 ctgcggcccc tgctggagaa gtgggtggag gaagccgaca acaatgagaa ccttcaggag
     2281 atatgcaaat cggagaccct ggtgcaggcc cggaagagaa agcgaactag cattgagaac
     2341 cgtgtgaggt ggagtctgga gaccatgttt ctgaagtgcc cgaagccctc cctacagcag
     2401 atcactcaca tcgccaatca gcttgggcta gagaaggatg tggttcgagt atggttctgt
     2461 aaccggcgcc agaagggcaa aagatcaagt attgagtatt cccaacgaga agagtatgag
     2521 gctacaggga cacctttccc agggggggct gtatcctttc ctctgccccc aggtccccac
     2581 tttggcaccc caggctatgg aagcccccac ttcaccacac tctactcagt cccttttcct
     2641 gagggcgagg cctttccctc tgttcccgtc actgctctgg gctctcccat gcattcaaac
     2701 ggggatctca aaattgtcgc tcctgtcaaa caaactctta actttgattt actcaaactg
     2761 gctggggatg tagaaagcaa tccaggtcca ctcatggctg tcagcgacgc tctgctcccg
     2821 tccttctcca cgttcgcgtc cggcccggcg ggaagggaga agacactgcg tccagcaggt
     2881 gccccgacta accgttggcg tgaggaactc tctcacatga agcgacttcc cccacttccc
     2941 ggccgcccct acgacctggc ggcgacggtg gccacagacc tggagagtgg cggagctggt
     3001 gcagcttgca gcagtaacaa cccggccctc ctagcccgga gggagaccga ggagttcaac
     3061 gacctcctgg acctagactt tatcctttcc aactcgctaa cccaccagga atcggtggcc
     3121 gccaccgtga ccacctcggc gtcagcttca tcctcgtctt ccccagcgag cagcggccct
     3181 gccagcgcgc cctccacctg cagcttcagc tatccgatcc gggccggggg tgacccgggc
     3241 gtggctgcca gcaacacagg tggagggctc ctctacagcc gagaatctgc gccacctccc
     3301 acggccccct tcaacctggc ggacatcaat gacgtgagcc cctcgggcgg cttcgtggct
     3361 gagctcctgc ggccggagtt ggacccagta tacattccgc cacagcagcc tcagccgcca
     3421 ggtggcgggc tgatgggcaa gtttgtgctg aaggcgtctc tgaccacccc tggcagcgag
     3481 tacagcagcc cttcggtcat cagtgttagc aaaggaagcc cagacggcag ccaccccgtg
     3541 gtagtggcgc cctacagcgg tggcccgccg cgcatgtgcc ccaagattaa gcaagaggcg
     3601 gtcccgtcct gcacggtcag ccggtcccta gaggcccatt tgagcgctgg accccagctc
     3661 agcaacggcc accggcccaa cacacacgac ttccccctgg ggcggcagct ccccaccagg
     3721 actaccccta cactgagtcc cgaggaactg ctgaacagca gggactgtca ccctggcctg
     3781 cctcttcccc caggattcca tccccatccg gggcccaact accctccttt cctgccagac
     3841 cagatgcagt cacaagtccc ctctctccat tatcaagagc tcatgccacc gggttcctgc
     3901 ctgccagagg agcccaagcc aaagagggga agaaggtcgt ggccccggaa aagaacagcc
     3961 acccacactt gtgactatgc aggctgtggc aaaacctata ccaagagttc tcatctcaag
     4021 gcacacctgc gaactcacac aggcgagaaa ccttaccact gtgactggga cggctgtggg
     4081 tggaaattcg cccgctccga tgaactgacc aggcactacc gcaaacacac agggcaccgg
     4141 ccctttcagt gccagaagtg cgacagggcc ttttccaggt cggaccacct tgccttacac
     4201 atgaagaggc actttctcga gggctcgggc cagtgtacta attatgctct cttgaaattg
     4261 gctggagatg ttgagagcaa cccaggtccc ggcgcgccac tggatttcct ttgggcgttg
     4321 gaaaccccgc agacagccac gacgatgccc ctcaacgtga acttcaccaa caggaactat
     4381 gacctcgact acgactccgt acagccctat ttcatctgcg acgaggaaga gaatttctat
     4441 caccagcaac agcagagcga gctgcagccg cccgcgccca gtgaggatat ctggaagaaa
     4501 ttcgagctgc ttcccacccc gcccctgtcc ccgagccgcc gctccgggct ctgctctcca
     4561 tcctatgttg cggtcgctac gtccttctcc ccaagggaag acgatgacgg cggcggtggc
     4621 aacttctcca ccgccgatca gctggagatg atgaccgagt tacttggagg agacatggtg
     4681 aaccagagct tcatctgcga tcctgacgac gagaccttca tcaagaacat catcatccag
     4741 gactgtatgt ggagcggttt ctcagccgct gccaagctgg tctcggagaa gctggcctcc
     4801 taccaggctg cgcgcaaaga cagcaccagc ctgagccccg cccgcgggca cagcgtctgc
     4861 tccacctcca gcctgtacct gcaggacctc accgccgccg cgtccgagtg cattgacccc
     4921 tcagtggtct ttccctaccc gctcaacgac agcagctcgc ccaaatcctg tacctcgtcc
     4981 gattccacgg ccttctctcc ttcctcggac tcgctgctgt cctccgagtc ctccccacgg
     5041 gccagccctg agcccctagt gctgcatgag gagacaccgc ccaccaccag cagcgactct
     5101 gaagaagagc aagaagatga ggaagaaatt gatgtggtgt ctgtggagaa gaggcaaacc
     5161 cctgccaaga ggtcggagtc gggctcatct ccatcccgag gccacagcaa acctccgcac
     5221 agcccactgg tcctcaagag gtgccacgtc tccactcacc agcacaacta cgccgcaccc
     5281 ccctccacaa ggaaggacta tccagctgcc aagagggcca agttggacag tggcagggtc
     5341 ctgaagcaga tcagcaacaa ccgcaagtgc tccagcccca ggtcctcaga cacggaggaa
     5401 aacgacaaga ggcggacaca caacgtcttg gaacgtcaga ggaggaacga gctgaagcgc
     5461 agcttttttg ccctgcgtga ccagatccct gaattggaaa acaacgaaaa ggcccccaag
     5521 gtagtgatcc tcaaaaaagc caccgcctac atcctgtcca ttcaagcaga cgagcacaag
     5581 ctcacctctg aaaaggactt attgaggaaa cgacgagaac agttgaaaca caaactcgaa
     5641 cagcttcgaa actctggtgc aggggatctc aaaattgtcg ctcctgtcaa acaaactctt
     5701 aactttgatt tactcaaact ggctggggat gtagaaagca atccaggtcc actcatgaac
     5761 aattcgccct tcaccatgta taacatgatg gagacggagc tgaagccgcc gggcccgcag
     5821 caagcttcgg ggggcggcgg cggaggaggc aacgccacgg cggcggcgac cggcggcaac
     5881 cagaagaaca gcccggaccg cgtcaagagg cccatgaacg ccttcatggt atggtcccgg
     5941 gggcagcggc gtaagatggc ccaggagaac cccaagatgc acaactcgga gatcagcaag
     6001 cgcctgggcg cggagtggaa acttttgtcc gagaccgaga agcggccgtt catcgacgag
     6061 gccaagcggc tgcgcgctct gcacatgaag gagcacccgg attataaata ccggccgcgg
     6121 cggaaaacca agacgctcat gaagaaggat aagtacacgc ttcccggagg cttgctggcc
     6181 cccggcggga acagcatggc gagcggggtt ggggtgggcg ccggcctggg tgcgggcgtg
     6241 aaccagcgca tggacagcta cgcgcacatg aacggctgga gcaacggcag ctacagcatg
     6301 atgcaggagc agctgggcta cccgcagcac ccgggcctca acgctcacgg cgcggcacag
     6361 atgcaaccga tgcaccgcta cgacgtcagc gccctgcagt acaactccat gaccagctcg
     6421 cagacctaca tgaacggctc gcccacctac agcatgtcct actcgcagca gggcaccccc
     6481 ggtatggcgc tgggctccat gggctctgtg gtcaagtccg aggccagctc cagccccccc
     6541 gtggttacct cttcctccca ctccagggcg ccctgccagg ccggggacct ccgggacatg
     6601 atcagcatgt acctccccgg cgccgaggtg ccggagcccg ctgcgcccag tagactgcac
     6661 atggcccagc actaccagag cggcccggtg cccggcacgg ccattaacgg cacactgccc
     6721 ctgtcgcaca tgtgagggct ggaagggcga attgcctgca ggaattcgcg gccgcactcg
     6781 agatatctag acccagcttt cttgtacaaa gtggtgatct agctaaaacg cggcctcgaa
     6841 tcgaattccg cccccccccc tctccctccc ccccccctaa cgttactggc cgaagccgct
     6901 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     6961 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     7021 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     7081 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     7141 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     7201 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     7261 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     7321 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aacgtctagg
     7381 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa
     7441 ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     7501 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     7561 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     7621 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     7681 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     7741 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     7801 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     7861 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     7921 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     7981 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     8041 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     8101 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag taaagcggcc
     8161 gcgatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga
     8221 cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc
     8281 tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg
     8341 tttagagttt ggcaacatat gccatatgct ggctgccatg aacaaaggtg gctataaaga
     8401 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
     8461 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
     8521 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
     8581 atagctgtcc ctcttctctt atgaagatcc ctcgacctgc aggaattcga ttacttaagc
     8641 ctaggatcca gatctgtaca ttcgaatcga tgaattcgtt aaccatggaa gcttctgcag
     8701 cggccgcact agaggtaccc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     8761 ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc cattgacgtc
     8821 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     8881 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tgtgcccagt
     8941 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     9001 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
     9061 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
     9121 ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga
     9181 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
     9241 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgacgc
     9301 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg
     9361 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag
     9421 ctgagcaaga ggtaagggtt taagggatgg ttggttggtg gggtattaat gtttaattac
     9481 ctggagcacc tgcctgaaat cacttttttt caggttggac cggtgccacc atggatgcta
     9541 agtcactaac tgcctggtcc cggacactgg tgaccttcaa ggatgtattt gtggacttca
     9601 ccagggagga gtggaagctg ctggacactg ctcagcagat cgtgtacaga aatgtgatgc
     9661 tggagaacta taagaacctg gtttccttgg gttatcagct tactaagcca gatgtgatcc
     9721 tccggttgga gaagggagaa gagcccggcg gttccggcgg agggtcgatg gacaagaagt
     9781 acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt
     9841 acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga
     9901 agaacctgat cggcgccctg ctgttcgaca gcggagaaac agccgaggcc acccggctga
     9961 agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga
    10021 tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct
    10081 tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg
    10141 aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca
    10201 gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc
    10261 ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt
    10321 tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg
    10381 gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc
    10441 tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggcaacctg attgccctga
    10501 gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc
    10561 agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc
    10621 agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca
    10681 tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat
    10741 acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg
    10801 agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacatcgatg
    10861 gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg
    10921 gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct
    10981 tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc
    11041 ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga
    11101 ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga
    11161 tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg
    11221 gcgccagcgc ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg
    11281 agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtac aacgagctga
    11341 ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga
    11401 aaaaagccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga
    11461 aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag
    11521 atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg
    11581 acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac
    11641 tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg
    11701 acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga
    11761 agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt
    11821 ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta
    11881 aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg
    11941 ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg
    12001 acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca
    12061 gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg
    12121 aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc
    12181 agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg
    12241 accaggaact ggacatcaac cggctgtccg actacgatgt ggacgctatc gtgcctcaga
    12301 gctttctgaa ggacgactcc atcgataaca aagtgctgac tcggagcgac aagaaccggg
    12361 gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcgcc
    12421 agctgctgaa tgccaagctg attacccaga ggaagttcga caatctgacc aaggccgaga
    12481 gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc
    12541 ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg
    12601 agaacgacaa actgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg
    12661 atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc
    12721 acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg
    12781 aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga
    12841 gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact
    12901 ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga
    12961 caaacggcga aacaggcgag atcgtgtggg ataagggccg ggactttgcc accgtgcgga
    13021 aagtgctgtc tatgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct
    13081 tcagcaaaga gtctatcctg cccaagagga acagcgacaa gctgatcgcc agaaagaagg
    13141 actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg
    13201 tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg
    13261 ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca
    13321 agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg
    13381 agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag aagggaaacg
    13441 aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc
    13501 tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaaacact
    13561 acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg
    13621 ctaatctgga caaggtgctg agcgcctaca acaagcacag agacaagcct atcagagagc
    13681 aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca
    13741 agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg
    13801 ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc
    13861 tgggaggcga cgcctatccc tatgacgtgc ccgattatgc cagcctgggc agcggctccc
    13921 ccaagaaaaa acgcaaggtg gaagatccta agaaaaagcg gaaagtggac ggcattggta
    13981 gtgggagcaa cggcagcagc ggatccgaat tcctagagct cgctgatcag cctcgactgt
    14041 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
    14101 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
    14161 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
    14221 agagaatagc aggcatgctg gggagcggcc gcactagtct agtctagagc gatcgcccgg
    14281 gtttaaacgc tagccaacaa gctcgtcatc gctttgcaga agagcagaga ggatatgctc
    14341 atcgtctaaa gaactaccca ttttattata tattagtcac gatatctata acaagaaaat
    14401 atatatataa taagttatca cgtaagtaga acatgaaata acaatataat tatcgtatga
    14461 gttaaatctt aaaagtcacg taaaagataa tcatgcgtca ttttgactca cgcggtcgtt
    14521 atagttcaaa atcagtgaca cttaccgcat tgacaagcac gcctcacggg agctccaagc
    14581 ggcgactgag atgtcctaaa tgcacagcga cggattcgcg ctatttagaa agagagagca
    14641 atatttcaag aatgcatgcg tcaattttac gcagactatc tttctagggt taaaaaagat
    14701 ttgcgcttta ctcgacctaa actttaaaca cgtcatagaa tcttcgtttg acaaaaacca
    14761 cattgtggcc aagctgtgtg acgcgacgcg cgctaaagaa tggcaaacca agtcgcgcga
    14821 ggtacccagc ttttgttccc tttagtgagg gttaattccg agcttggcgt aatcatggtc
    14881 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
    14941 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
    15001 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
    15061 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
    15121 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    15181 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    15241 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    15301 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    15361 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    15421 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    15481 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    15541 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    15601 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    15661 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    15721 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    15781 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    15841 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    15901 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    15961 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    16021 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    16081 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    16141 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    16201 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    16261 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    16321 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    16381 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    16441 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    16501 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    16561 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    16621 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    16681 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    16741 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    16801 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    16861 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    16921 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    16981 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
//
