LOCUS       sequence-231542-       17054 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac transposon for dox-inducible expression of the OK+9MS 
            (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming 
            cassette (with mCherry). KRAB-dCas9 is constitutively expressed..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17054)
  AUTHORS   Kagawa H, Shimamoto R, Kim SI, Oceguera-Yanez F, Yamamoto T, 
            Schroeder T, Woltjen K
  TITLE     OVOL1 Influences the Determination and Expansion of iPSC 
            Reprogramming Intermediates.
  JOURNAL   Stem Cell Reports. 2018 Dec 28. pii: S2213-6711(18)30527-7. doi: 
            10.1016/j.stemcr.2018.12.008.
  PUBMED    30639212
REFERENCE   2  (bases 1 to 17054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2018 Dec 28. pii: S2213-6711(18)30527-7. doi: 
            10.1016/j.stemcr.2018.12.008."
COMMENT     Sequence Label: PB-TAC-OK+9MS-KRAB-dCas9
FEATURES             Location/Qualifiers
     source          1..17054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   711..773
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1145..1163
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1182..1200
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1219..1237
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1256..1274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1293..1311
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1330..1348
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1367..1385
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1404..1422
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1444..1468
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1548..1572
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2725..2790
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             4255..4314
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             5710..5775
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     protein_bind    complement(6815..6839)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(6881..7454,7455..7457,7458..7466)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7048..7065)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7275..7294
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7467..8177
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7612..7630)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7880..7899
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(8189..8208)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8254..8309
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8308..8327)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     enhancer        8744..9029
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        9031..9308
                     /label=chicken beta-actin promoter
     intron          9309..9537
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      9549..9558
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9585..9770
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     CDS             join(9792..9818,9819..9821,9822..12308,12309..12311,
                     12312..13895)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9792..13895
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             13899..13925
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             13905..13931
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             13944..13964
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             13971..13991
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(14055..14072)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    14061..14268
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   14679..14713
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(14863..14881)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(14902..14918)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14902..14918)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14915..14937)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14926..14942
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14950..14956,14957..14974,14975..14980))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14995..15016
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15133..15150)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15304..15892)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15384..15403)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16063..16854,16855..16923))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16686..16705
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16924..17028)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcgg
      661 tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa ccctagaaag
      721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta
      781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt
      841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa
      901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct
      961 tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt
     1021 aattagctta acctatacat aatagaaaca aattatactt attagtcagt cagaaacaac
     1081 tttggcacat atcaatatta tgctctcgtt aattaactcg agggatccaa gctggccgca
     1141 cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca gtgatagaga
     1201 aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct
     1261 atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac
     1321 gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat
     1381 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtaccc cctatataag
     1441 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     1501 ccatagaaga caccgggacc gatccagcct ccgcggtggc ggccatcaca agtttgtaca
     1561 aaaaagcagg ctggcgccgg aaccaattca gtcgactgga tccggtaccg aattcgccct
     1621 taaaaagcag gctccacctt ccccatggct ggacacctgg cttcagactt cgccttctca
     1681 cccccaccag gtgggggtga tgggtcagca gggctggagc cgggctgggt ggattctcga
     1741 acctggctaa gcttccaagg gcctccaggt gggcctggaa tcggaccagg ctcagaggta
     1801 ttggggatct ccccatgtcc gcccgcatac gagttctgcg gagggatggc atactgtgga
     1861 cctcaggttg gactgggcct agtcccccaa gttggcgtgg agactttgca gcctgagggc
     1921 caggcaggag cacgagtgga aagcaactca gagggaacct cctctgagcc ctgtgccgac
     1981 cgccccaatg ccgtgaagtt ggagaaggtg gaaccaactc ccgaggagtc ccaggacatg
     2041 aaagccctgc agaaggagct agaacagttt gccaagctgc tgaagcagaa gaggatcacc
     2101 ttggggtaca cccaggccga cgtggggctc accctgggcg ttctctttgg aaaggtgttc
     2161 agccagacca ccatctgtcg cttcgaggcc ttgcagctca gccttaagaa catgtgtaag
     2221 ctgcggcccc tgctggagaa gtgggtggag gaagccgaca acaatgagaa ccttcaggag
     2281 atatgcaaat cggagaccct ggtgcaggcc cggaagagaa agcgaactag cattgagaac
     2341 cgtgtgaggt ggagtctgga gaccatgttt ctgaagtgcc cgaagccctc cctacagcag
     2401 atcactcaca tcgccaatca gcttgggcta gagaaggatg tggttcgagt atggttctgt
     2461 aaccggcgcc agaagggcaa aagatcaagt attgagtatt cccaacgaga agagtatgag
     2521 gctacaggga cacctttccc agggggggct gtatcctttc ctctgccccc aggtccccac
     2581 tttggcaccc caggctatgg aagcccccac ttcaccacac tctactcagt cccttttcct
     2641 gagggcgagg cctttccctc tgttcccgtc actgctctgg gctctcccat gcattcaaac
     2701 ggggatctca aaattgtcgc tcctgtcaaa caaactctta actttgattt actcaaactg
     2761 gctggggatg tagaaagcaa tccaggtcca atgaggcagc cacctggcga gtctgacatg
     2821 gctgtcagcg acgctctgct cccgtccttc tccacgttcg cgtccggccc ggcgggaagg
     2881 gagaagacac tgcgtccagc aggtgccccg actaaccgtt ggcgtgagga actctctcac
     2941 atgaagcgac ttcccccact tcccggccgc ccctacgacc tggcggcgac ggtggccaca
     3001 gacctggaga gtggcggagc tggtgcagct tgcagcagta acaacccggc cctcctagcc
     3061 cggagggaga ccgaggagtt caacgacctc ctggacctag actttatcct ttccaactcg
     3121 ctaacccacc aggaatcggt ggccgccacc gtgaccacct cggcgtcagc ttcatcctcg
     3181 tcttccccag cgagcagcgg ccctgccagc gcgccctcca cctgcagctt cagctatccg
     3241 atccgggccg ggggtgaccc gggcgtggct gccagcaaca caggtggagg gctcctctac
     3301 agccgagaat ctgcgccacc tcccacggcc cccttcaacc tggcggacat caatgacgtg
     3361 agcccctcgg gcggcttcgt ggctgagctc ctgcggccgg agttggaccc agtatacatt
     3421 ccgccacagc agcctcagcc gccaggtggc gggctgatgg gcaagtttgt gctgaaggcg
     3481 tctctgacca cccctggcag cgagtacagc agcccttcgg tcatcagtgt tagcaaagga
     3541 agcccagacg gcagccaccc cgtggtagtg gcgccctaca gcggtggccc gccgcgcatg
     3601 tgccccaaga ttaagcaaga ggcggtcccg tcctgcacgg tcagccggtc cctagaggcc
     3661 catttgagcg ctggacccca gctcagcaac ggccaccggc ccaacacaca cgacttcccc
     3721 ctggggcggc agctccccac caggactacc cctacactga gtcccgagga actgctgaac
     3781 agcagggact gtcaccctgg cctgcctctt cccccaggat tccatcccca tccggggccc
     3841 aactaccctc ctttcctgcc agaccagatg cagtcacaag tcccctctct ccattatcaa
     3901 gagctcatgc caccgggttc ctgcctgcca gaggagccca agccaaagag gggaagaagg
     3961 tcgtggcccc ggaaaagaac agccacccac acttgtgact atgcaggctg tggcaaaacc
     4021 tataccaaga gttctcatct caaggcacac ctgcgaactc acacaggcga gaaaccttac
     4081 cactgtgact gggacggctg tgggtggaaa ttcgcccgct ccgatgaact gaccaggcac
     4141 taccgcaaac acacagggca ccggcccttt cagtgccaga agtgcgacag ggccttttcc
     4201 aggtcggacc accttgcctt acacatgaag aggcactttc tcgagggctc gggccagtgt
     4261 actaattatg ctctcttgaa attggctgga gatgttgaga gcaacccagg tcccggcgcg
     4321 ccactggatt tcctttgggc gttggaaacc ccgcagacag ccacgacgat gcccctcaac
     4381 gtgaacttca ccaacaggaa ctatgacctc gactacgact ccgtacagcc ctatttcatc
     4441 tgcgacgagg aagagaattt ctatcaccag caacagcaga gcgagctgca gccgcccgcg
     4501 cccagtgagg atatctggaa gaaattcgag ctgcttccca ccccgcccct gtccccgagc
     4561 cgccgctccg ggctctgctc tccatcctat gttgcggtcg ctacgtcctt ctccccaagg
     4621 gaagacgatg acggcggcgg tggcaacttc tccaccgccg atcagctgga gatgatgacc
     4681 gagttacttg gaggagacat ggtgaaccag agcttcatct gcgatcctga cgacgagacc
     4741 ttcatcaaga acatcatcat ccaggactgt atgtggagcg gtttctcagc cgctgccaag
     4801 ctggtctcgg agaagctggc ctcctaccag gctgcgcgca aagacagcac cagcctgagc
     4861 cccgcccgcg ggcacagcgt ctgctccacc tccagcctgt acctgcagga cctcaccgcc
     4921 gccgcgtccg agtgcattga cccctcagtg gtctttccct acccgctcaa cgacagcagc
     4981 tcgcccaaat cctgtacctc gtccgattcc acggccttct ctccttcctc ggactcgctg
     5041 ctgtcctccg agtcctcccc acgggccagc cctgagcccc tagtgctgca tgaggagaca
     5101 ccgcccacca ccagcagcga ctctgaagaa gagcaagaag atgaggaaga aattgatgtg
     5161 gtgtctgtgg agaagaggca aacccctgcc aagaggtcgg agtcgggctc atctccatcc
     5221 cgaggccaca gcaaacctcc gcacagccca ctggtcctca agaggtgcca cgtctccact
     5281 caccagcaca actacgccgc acccccctcc acaaggaagg actatccagc tgccaagagg
     5341 gccaagttgg acagtggcag ggtcctgaag cagatcagca acaaccgcaa gtgctccagc
     5401 cccaggtcct cagacacgga ggaaaacgac aagaggcgga cacacaacgt cttggaacgt
     5461 cagaggagga acgagctgaa gcgcagcttt tttgccctgc gtgaccagat ccctgaattg
     5521 gaaaacaacg aaaaggcccc caaggtagtg atcctcaaaa aagccaccgc ctacatcctg
     5581 tccattcaag cagacgagca caagctcacc tctgaaaagg acttattgag gaaacgacga
     5641 gaacagttga aacacaaact cgaacagctt cgaaactctg gtgcagggga tctcaaaatt
     5701 gtcgctcctg tcaaacaaac tcttaacttt gatttactca aactggctgg ggatgtagaa
     5761 agcaatccag gtccactcat gaacaattcg cccttcacca tgtataacat gatggagacg
     5821 gagctgaagc cgccgggccc gcagcaagct tcggggggcg gcggcggagg aggcaacgcc
     5881 acggcggcgg cgaccggcgg caaccagaag aacagcccgg accgcgtcaa gaggcccatg
     5941 aacgccttca tggtatggtc ccgggggcag cggcgtaaga tggcccagga gaaccccaag
     6001 atgcacaact cggagatcag caagcgcctg ggcgcggagt ggaaactttt gtccgagacc
     6061 gagaagcggc cgttcatcga cgaggccaag cggctgcgcg ctctgcacat gaaggagcac
     6121 ccggattata aataccggcc gcggcggaaa accaagacgc tcatgaagaa ggataagtac
     6181 acgcttcccg gaggcttgct ggcccccggc gggaacagca tggcgagcgg ggttggggtg
     6241 ggcgccggcc tgggtgcggg cgtgaaccag cgcatggaca gctacgcgca catgaacggc
     6301 tggagcaacg gcagctacag catgatgcag gagcagctgg gctacccgca gcacccgggc
     6361 ctcaacgctc acggcgcggc acagatgcaa ccgatgcacc gctacgacgt cagcgccctg
     6421 cagtacaact ccatgaccag ctcgcagacc tacatgaacg gctcgcccac ctacagcatg
     6481 tcctactcgc agcagggcac ccccggtatg gcgctgggct ccatgggctc tgtggtcaag
     6541 tccgaggcca gctccagccc ccccgtggtt acctcttcct cccactccag ggcgccctgc
     6601 caggccgggg acctccggga catgatcagc atgtacctcc ccggcgccga ggtgccggag
     6661 cccgctgcgc ccagtagact gcacatggcc cagcactacc agagcggccc ggtgcccggc
     6721 acggccatta acggcacact gcccctgtcg cacatgtgag ggctggaagg gcgaattgcc
     6781 tgcaggaatt cgcggccgca ctcgagatat ctagacccag ctttcttgta caaagtggtg
     6841 atctagctaa aacgcggcct cgaatcgaat tccgcccccc cccctctccc tccccccccc
     6901 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     6961 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     7021 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     7081 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     7141 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     7201 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     7261 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     7321 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     7381 agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     7441 aacacgatga taatatggcc acaaccatgg tgagcaaggg cgaggaggat aacatggcca
     7501 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
     7561 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     7621 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
     7681 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
     7741 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     7801 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
     7861 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
     7921 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
     7981 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
     8041 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
     8101 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
     8161 acgagctgta caagtaaagc ggccgcgatc tttttccctc tgccaaaaat tatggggaca
     8221 tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa
     8281 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt
     8341 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgccata tgctggctgc
     8401 catgaacaaa ggtggctata aagaggtcat cagtatatga aacagccccc tgctgtccat
     8461 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg
     8521 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc
     8581 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatgaag atccctcgac
     8641 ctgcaggaat tcgattactt aagcctagga tccagatctg tacattcgaa tcgatgaatt
     8701 cgttaaccat ggaagcttct gcagcggccg cactagaggt acccgttaca taacttacgg
     8761 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca atagtaacgc
     8821 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     8881 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     8941 ggcccgcctg gcattgtgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     9001 tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc
     9061 tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt
     9121 gtgcagcgat gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg
     9181 aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc
     9241 gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc
     9301 ggcgggcggg agtcgctgcg acgctgcctt cgccccgtgc cccgctccgc cgccgcctcg
     9361 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
     9421 ccttctcctc cgggctgtaa ttagctgagc aagaggtaag ggtttaaggg atggttggtt
     9481 ggtggggtat taatgtttaa ttacctggag cacctgcctg aaatcacttt ttttcaggtt
     9541 ggaccggtgc caccatggat gctaagtcac taactgcctg gtcccggaca ctggtgacct
     9601 tcaaggatgt atttgtggac ttcaccaggg aggagtggaa gctgctggac actgctcagc
     9661 agatcgtgta cagaaatgtg atgctggaga actataagaa cctggtttcc ttgggttatc
     9721 agcttactaa gccagatgtg atcctccggt tggagaaggg agaagagccc ggcggttccg
     9781 gcggagggtc gatggacaag aagtacagca tcggcctggc catcggcacc aactctgtgg
     9841 gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca
     9901 acaccgaccg gcacagcatc aagaagaacc tgatcggcgc cctgctgttc gacagcggag
     9961 aaacagccga ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga
    10021 accggatctg ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct
    10081 tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc
    10141 ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc
    10201 acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg
    10261 ccctggccca catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg
    10321 acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg
    10381 aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga
    10441 gcaagagcag acggctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc
    10501 tgttcggcaa cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg
    10561 acctggccga ggatgccaaa ctgcagctga gcaaggacac ctacgacgac gacctggaca
    10621 acctgctggc ccagatcggc gaccagtacg ccgacctgtt tctggccgcc aagaacctgt
    10681 ccgacgccat cctgctgagc gacatcctga gagtgaacac cgagatcacc aaggcccccc
    10741 tgagcgcctc tatgatcaag agatacgacg agcaccacca ggacctgacc ctgctgaaag
    10801 ctctcgtgcg gcagcagctg cctgagaagt acaaagagat tttcttcgac cagagcaaga
    10861 acggctacgc cggctacatc gatggcggag ccagccagga agagttctac aagttcatca
    10921 agcccatcct ggaaaagatg gacggcaccg aggaactgct cgtgaagctg aacagagagg
    10981 acctgctgcg gaagcagcgg accttcgaca acggcagcat cccccaccag atccacctgg
    11041 gagagctgca cgccattctg cggcggcagg aagattttta cccattcctg aaggacaacc
    11101 gggaaaagat cgagaagatc ctgaccttcc gcatccccta ctacgtgggc cctctggcca
    11161 ggggaaacag cagattcgcc tggatgacca gaaagagcga ggaaaccatc accccctgga
    11221 acttcgagga agtggtggac aagggcgcca gcgcccagag cttcatcgag cggatgacca
    11281 acttcgataa gaacctgccc aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt
    11341 acttcaccgt gtacaacgag ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc
    11401 ccgccttcct gagcggcgag cagaaaaaag ccatcgtgga cctgctgttc aagaccaacc
    11461 ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag tgcttcgact
    11521 ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca taccacgatc
    11581 tgctgaaaat tatcaaggac aaggacttcc tggacaatga ggaaaacgag gacattctgg
    11641 aagatatcgt gctgaccctg acactgtttg aggacagaga gatgatcgag gaacggctga
    11701 aaacctatgc ccacctgttc gacgacaaag tgatgaagca gctgaagcgg cggagataca
    11761 ccggctgggg caggctgagc cggaagctga tcaacggcat ccgggacaag cagtccggca
    11821 agacaatcct ggatttcctg aagtccgacg gcttcgccaa cagaaacttc atgcagctga
    11881 tccacgacga cagcctgacc tttaaagagg acatccagaa agcccaggtg tccggccagg
    11941 gcgatagcct gcacgagcac attgccaatc tggccggcag ccccgccatt aagaagggca
    12001 tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg
    12061 agaacatcgt gatcgaaatg gccagagaga accagaccac ccagaaggga cagaagaaca
    12121 gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc cagatcctga
    12181 aagaacaccc cgtggaaaac acccagctgc agaacgagaa gctgtacctg tactacctgc
    12241 agaatgggcg ggatatgtac gtggaccagg aactggacat caaccggctg tccgactacg
    12301 atgtggacgc tatcgtgcct cagagctttc tgaaggacga ctccatcgat aacaaagtgc
    12361 tgactcggag cgacaagaac cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga
    12421 agaagatgaa gaactactgg cgccagctgc tgaatgccaa gctgattacc cagaggaagt
    12481 tcgacaatct gaccaaggcc gagagaggcg gcctgagcga actggataag gccggcttca
    12541 tcaagagaca gctggtggaa acccggcaga tcacaaagca cgtggcacag atcctggact
    12601 cccggatgaa cactaagtac gacgagaacg acaaactgat ccgggaagtg aaagtgatca
    12661 ccctgaagtc caagctggtg tccgatttcc ggaaggattt ccagttttac aaagtgcgcg
    12721 agatcaacaa ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc
    12781 tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta cggcgactac aaggtgtacg
    12841 acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg caaggctacc gccaagtact
    12901 tcttctacag caacatcatg aactttttca agaccgagat taccctggcc aacggcgaga
    12961 tccggaagcg gcctctgatc gagacaaacg gcgaaacagg cgagatcgtg tgggataagg
    13021 gccgggactt tgccaccgtg cggaaagtgc tgtctatgcc ccaagtgaat atcgtgaaaa
    13081 agaccgaggt gcagacaggc ggcttcagca aagagtctat cctgcccaag aggaacagcg
    13141 acaagctgat cgccagaaag aaggactggg accctaagaa gtacggcggc ttcgacagcc
    13201 ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac
    13261 tgaagagtgt gaaagagctg ctggggatca ccatcatgga aagaagcagc ttcgagaaga
    13321 atcccatcga ctttctggaa gccaagggct acaaagaagt gaaaaaggac ctgatcatca
    13381 agctgcctaa gtactccctg ttcgagctgg aaaacggccg gaagagaatg ctggcctctg
    13441 ccggcgaact gcagaaggga aacgaactgg ccctgccctc caaatatgtg aacttcctgt
    13501 acctggccag ccactatgag aagctgaagg gctcccccga ggataatgag cagaaacagc
    13561 tgtttgtgga acagcacaaa cactacctgg acgagatcat cgagcagatc agcgagttct
    13621 ccaagagagt gatcctggcc gacgctaatc tggacaaggt gctgagcgcc tacaacaagc
    13681 acagagacaa gcctatcaga gagcaggccg agaatatcat ccacctgttt accctgacca
    13741 atctgggagc ccctgccgcc ttcaagtact ttgacaccac catcgaccgg aagaggtaca
    13801 ccagcaccaa agaggtgctg gacgccaccc tgatccacca gagcatcacc ggcctgtacg
    13861 agacacggat cgacctgtct cagctgggag gcgacgccta tccctatgac gtgcccgatt
    13921 atgccagcct gggcagcggc tcccccaaga aaaaacgcaa ggtggaagat cctaagaaaa
    13981 agcggaaagt ggacggcatt ggtagtggga gcaacggcag cagcggatcc gaattcctag
    14041 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
    14101 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
    14161 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
    14221 ggacagcaag ggggaggatt gggaagagaa tagcaggcat gctggggagc ggccgcacta
    14281 gtctagtcta gagcgatcgc ccgggtttaa acgctagcca acaagctcgt catcgctttg
    14341 cagaagagca gagaggatat gctcatcgtc taaagaacta cccattttat tatatattag
    14401 tcacgatatc tataacaaga aaatatatat ataataagtt atcacgtaag tagaacatga
    14461 aataacaata taattatcgt atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc
    14521 gtcattttga ctcacgcggt cgttatagtt caaaatcagt gacacttacc gcattgacaa
    14581 gcacgcctca cgggagctcc aagcggcgac tgagatgtcc taaatgcaca gcgacggatt
    14641 cgcgctattt agaaagagag agcaatattt caagaatgca tgcgtcaatt ttacgcagac
    14701 tatctttcta gggttaaaaa agatttgcgc tttactcgac ctaaacttta aacacgtcat
    14761 agaatcttcg tttgacaaaa accacattgt ggccaagctg tgtgacgcga cgcgcgctaa
    14821 agaatggcaa accaagtcgc gcgaggtacc cagcttttgt tccctttagt gagggttaat
    14881 tccgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
    14941 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
    15001 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
    15061 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
    15121 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
    15181 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
    15241 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
    15301 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    15361 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    15421 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    15481 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    15541 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    15601 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    15661 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    15721 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
    15781 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    15841 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    15901 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    15961 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    16021 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    16081 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    16141 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    16201 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    16261 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    16321 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    16381 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    16441 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    16501 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    16561 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    16621 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    16681 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    16741 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    16801 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    16861 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    16921 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    16981 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    17041 aaaagtgcca cctg
//
