LOCUS       sequence-231551-       10198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive lentiviral expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10198)
  AUTHORS   Li J, Choi PS, Chaffer CL, Labella K, Hwang JH, Giacomelli AO, Kim 
            JW, Ilic N, Doench JG, Ly SH, Dai C, Hagel K, Hong AL, Gjoerup O, 
            Goel S, Ge JY, Root DE, Zhao JJ, Brooks AN, Weinberg RA, Hahn WC
  TITLE     An alternative splicing switch in FLNB promotes the mesenchymal cell
            state in human breast cancer.
  JOURNAL   Elife. 2018 Jul 30;7. pii: 37184. doi: 10.7554/eLife.37184.
  PUBMED    30059005
REFERENCE   2  (bases 1 to 10198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-07-30"; volume: "7. pii: 37184. doi"; pages: " 
            10.7554/eLife.37184"
COMMENT     Sequence Label: pLX317-hcRED
FEATURES             Location/Qualifiers
     source          1..10198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    20..44
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             47..730
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     protein_bind    complement(732..756)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             764..805
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    916..1504
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(969..989)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1387..1398)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1576..1809
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1887..2008
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1924..1943)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1978..1997
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2048..2183
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2203..2222)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2204..2222)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2232..2249)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2232..2248)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2241..2263)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2390..2845
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2477..2496)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2687..2708
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2871..2975
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2976..3044,3045..3836)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3194..3213)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     protein_bind    4853..4874
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4889..4894,4895..4912,4913..4919)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4923..4946
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     rep_origin      5048..5636
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5924..5945
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5960..5965,5966..5983,5984..5990)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5998..6014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6003..6025
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6022..6038
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6022..6038
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6057..6077
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6059..6077
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6105..6331
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6332..6512
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6559..6684
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7177..7410
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7595..7639
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7870..8080
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7892..8088
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7939..8074
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8120..8722
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8120..8139)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8616..8636
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8765..8882
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        9020..10198
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          9251..10189
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     10146..10166
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ggctagcatc gattgatcaa caagtttgta caaaaaagtt ggcaccatgg tgagcggcct
       61 gctgaaggag agtatgcgca tcaagatgta catggagggc accgtgaacg gccactactt
      121 caagtgcgag ggcgagggcg acggcaaccc cttcgccggc acccagagca tgagaatcca
      181 cgtgaccgag ggcgcccccc tgcccttcgc cttcgacatc ctggccccct gctgcgagta
      241 cggcagcagg accttcgtgc accacaccgc cgagatcccc gacttcttca agcagagctt
      301 ccccgagggc ttcacctggg agagaaccac cacctacgag gacggcggca tcctgaccgc
      361 ccaccaggac accagcctgg agggcaactg cctgatctac aaggtgaagg tgcacggcac
      421 caacttcccc gccgacggcc ccgtgatgaa gaacaagagc ggcggctggg agcccagcac
      481 cgaggtggtg taccccgaga acggcgtgct gtgcggccgg aacgtgatgg ccctgaaggt
      541 gggcgaccgg cacctgatct gccaccacta caccagctac cggagcaaga aggccgtgcg
      601 cgccctgacc atgcccggct tccacttcac cgacatccgg ctccagatgc tgcggaagaa
      661 gaaggacgag tacttcgagc tgtacgaggc cagcgtggcc cggtacagcg acctgcccga
      721 gaaggccaac ttgccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa
      781 ccctctcctc ggtctcgatt ctacgtagta atgaactagt ccgtaacttg aaagtatttc
      841 gatttcttgg ctttatatat cttgtggaaa ggacgatgct atcaccaccg tacaggtccc
      901 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
      961 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     1021 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     1081 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     1141 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     1201 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     1261 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     1321 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     1381 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     1441 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     1501 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     1561 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     1621 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     1681 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     1741 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     1801 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     1861 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     1921 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     1981 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     2041 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     2101 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     2161 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     2221 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     2281 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     2341 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     2401 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     2461 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     2521 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     2581 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     2641 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     2701 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     2761 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     2821 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     2881 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     2941 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     3001 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     3061 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     3121 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     3181 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     3241 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     3301 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     3361 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     3421 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     3481 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     3541 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     3601 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     3661 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     3721 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     3781 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     3841 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     3901 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     3961 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     4021 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     4081 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     4141 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     4201 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     4261 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     4321 gctgaacggg gggttcgtgc acacagccca gcttcctaca gcgtgagcta tgagaaagcg
     4381 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4441 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4501 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4561 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4621 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     4681 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     4741 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     4801 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     4861 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     4921 gtggaattgt gagcggataa caattctagg tgaagatcct ttttgataat ctcatgacca
     4981 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5041 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     5101 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5161 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     5221 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     5281 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     5341 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     5401 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     5461 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     5521 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     5581 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     5641 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     5701 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     5761 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     5821 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     5881 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     5941 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     6001 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     6061 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     6121 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     6181 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     6241 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     6301 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     6361 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     6421 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     6481 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     6541 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     6601 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     6661 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     6721 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     6781 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     6841 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     6901 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     6961 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     7021 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     7081 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     7141 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     7201 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     7261 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     7321 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     7381 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     7441 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     7501 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     7561 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     7621 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     7681 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     7741 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     7801 cccgagggga cccatgcatt gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     7861 ggctccccag caggcagaag tatgcaaagc atgcgtctca attagtcagc aaccatagtc
     7921 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     7981 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     8041 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctttctaga
     8101 ggtaccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     8161 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     8221 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     8281 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgccgtggc
     8341 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     8401 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     8461 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     8521 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     8581 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     8641 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     8701 ccgcaagccc ggtgccagtt aacgaattct agatcttgag acaaatggca gtattcatcc
     8761 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     8821 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     8881 ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag gcctgcaggt
     8941 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     9001 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     9061 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     9121 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     9181 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     9241 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     9301 cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct
     9361 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
     9421 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
     9481 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
     9541 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
     9601 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
     9661 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
     9721 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
     9781 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
     9841 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
     9901 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
     9961 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
    10021 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
    10081 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
    10141 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtga
//
