LOCUS       sequence-231552-       11352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of PFKP-GFP fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11352)
  AUTHORS   Park JS, Burckhardt CJ, Lazcano R, Solis LM, Isogai T, Li L, Chen 
            CS, Gao B, Minna JD, Bachoo R, DeBerardinis RJ, Danuser G
  TITLE     Mechanical regulation of glycolysis via cytoskeleton architecture.
  JOURNAL   Nature. 2020 Feb 12. pii: 10.1038/s41586-020-1998-1. doi: 
            10.1038/s41586-020-1998-1.
  PUBMED    32051585
REFERENCE   2  (bases 1 to 11352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2020 Feb 12. pii: 10.1038/s41586-020-1998-1. doi: 
            10.1038/s41586-020-1998-1."
COMMENT     Sequence Label: pLVX neo PFKP-GFP
FEATURES             Location/Qualifiers
     source          1..11352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             118..751
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    798..923
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1420..1653
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1838..1882
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2145..2260
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2318..2621
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2622..2825
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2776..2796
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2822..2846
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5300..5309
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5306..5308,5309..5311,5312..6025)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5351..5372)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5612..5631)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5959..5980
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(6043..6617,6618..6620,6621..6629)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6210..6227)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6437..6456
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6630..7424
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6684..6703)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7294..7313
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7438..8026
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7491..7511)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7909..7920)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8233..8866
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8994..9010)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8994..9010)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9007..9029)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9018..9034
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9042..9048,9049..9066,9067..9072))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9087..9108
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9225..9242)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9396..9984)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9476..9495)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10155..10946,10947..11015))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10778..10797
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11016..11120)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11168..11302
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11205..11224)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11259..11278
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
       61 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg
      121 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
      181 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
      241 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
      301 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
      361 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
      421 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
      481 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
      541 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
      601 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
      661 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
      721 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
      781 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
      841 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
      901 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
      961 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     1021 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     1081 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     1141 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     1201 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     1261 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1321 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1381 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1441 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1501 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1561 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1621 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1681 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1741 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1801 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1861 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1921 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1981 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     2041 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     2101 agacagatcc attcgattag tgaacggatc tcgacggtat cgcctttaaa agaaaagggg
     2161 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2221 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2281 gcagagatcc agtttatcga taagcttggg agttccgcgt tacataactt acggtaaatg
     2341 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     2401 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     2461 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     2521 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     2581 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     2641 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     2701 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     2761 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     2821 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
     2881 atagaagaca ccgactctac tagaggatct atttccggtg aattctttcg agatggacgc
     2941 ggacgactcc cgggccccca agggctcctt gcggaagttc ctggagcacc tctccggggc
     3001 cggcaaggcc atcggcgtgc tgaccagcgg cggggatgct caaggtatga acgctgccgt
     3061 ccgtgccgtg gtgcgcatgg gtatctacgt gggggccaag gtgtacttca tctacgaggg
     3121 ctaccagggc atggtggacg gaggctcaaa catcgcagag gccgactggg agagtgtctc
     3181 cagcatcctg caagtgggcg ggacgatcat tggcagtgcg cggtgccagg ccttccgcac
     3241 gcgggaaggc cgcctgaagg ctgcttgcaa cctgctgcag cgcggcatca ccaacctgtg
     3301 tgtgatcggc ggggacggga gcctcaccgg ggccaacctc ttccggaagg agtggagtgg
     3361 gctgctggag gagctggcca ggaacggcca gatcgataag gaggccgtgc agaagtacgc
     3421 ctacctcaac gtggtgggca tggtgggctc catcgacaat gatttctgcg gcaccgacat
     3481 gaccatcggc acggactccg ccctgcacag gatcatcgag gtcgtcgacg ccatcatgac
     3541 cacggcccag agccaccaga ggaccttcgt tctggaggtg atgggacgac actgtgggta
     3601 cctggccctg gtgagtgcct tggcctgcgg tgcggactgg gtgttccttc cagaatctcc
     3661 accagaggaa ggctgggagg agcagatgtg tgtcaaactc tcggagaacc gtgcccggaa
     3721 aaaaaggctg aatattatta ttgtggctga aggagcaatt gatacccaaa ataaacccat
     3781 cacctctgag aaaatcaaag agcttgtcgt cacgcagctg ggctatgaca cacgtgtgac
     3841 catcctcggg cacgtgcaga gaggagggac cccttcggca ttcgacagga tcttggccag
     3901 ccgcatggga gtggaggcag tcatcgcctt gctagaggcc accccggaca ccccagcttg
     3961 cgtcgtgtca ctgaacggga accacgccgt gcgcctgccg ctgatggagt gcgtgcagat
     4021 gactcaggat gtgcagaagg cgatggacga gaggagattt caagatgcgg ttcgactccg
     4081 agggaggagc tttgcgggca acctgaacac ctacaagcga cttgccatca agctgccgga
     4141 tgatcagatc ccaaagacca attgcaacgt agctgtcatc aacgtggggg cacccgcggc
     4201 tgggatgaac gcggccgtac gctcagctgt gcgcgtgggc attgccgacg gccacaggat
     4261 gctcgccatc tatgatggct ttgacggctt cgccaagggc cagatcaaag aaatcggctg
     4321 gacagatgtc gggggctgga ccggccaagg aggctccatt cttgggacaa aacgcgttct
     4381 cccggggaag tacttggaag agatcgccac acagatgcgc acgcacagca tcaacgcgct
     4441 gctgatcatc ggtggattcg aggcctacct gggactcctg gagctgtcag ccgcccggga
     4501 gaagcacgag gagttctgtg tccccatggt catggttccc gctactgtgt ccaacaatgt
     4561 gccgggttcc gatttcagca tcggggcaga caccgccctg aacactatca ccgacacctg
     4621 cgaccgcatc aagcagtccg ccagcggaac caagcggcgc gtgttcatca tcgagaccat
     4681 gggcggctac tgtggctacc tggccaacat gggggggctc gcggccggag ctgatgccgc
     4741 atacattttc gaagagccct tcgacatcag ggatctgcag tccaacgtgg agcacctgac
     4801 ggagaaaatg aagaccacca tccagagagg ccttgtgctc agaaatgaga gctgcagtga
     4861 aaactacacc accgacttca tttaccagct gtattcagaa gagggcaaag gcgtgtttga
     4921 ctgcaggaag aacgtgctgg gtcacatgca gcagggtggg gcaccctctc catttgatag
     4981 aaactttgga accaaaatct ctgccagagc tatggagtgg atcactgcaa aactcaagga
     5041 ggcccggggc agaggaaaaa aatttaccac cgatgattcc atttgtgtgc tgggaataag
     5101 caaaagaaac gttatttttc aacctgtggc agagctgaag aagcaaacgg attttgagca
     5161 caggattccc aaagaacagt ggtggctcaa gctacggccc ctcatgaaaa tcctggccaa
     5221 gtacaaggcc agctatgacg tgtcggactc aggccagctg gaacatgtgc agccctggag
     5281 tgtcccggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     5341 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     5401 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     5461 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     5521 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     5581 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     5641 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     5701 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     5761 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     5821 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     5881 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc
     5941 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     6001 cggcatggac gagctgtaca agtaaagcgg ccgcggatcc cgcccctctc cctccccccc
     6061 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     6121 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     6181 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     6241 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     6301 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     6361 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     6421 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     6481 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     6541 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     6601 aaaaacacga tgataagctt gccacaacca tggctgaaca agatggattg cacgcaggtt
     6661 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     6721 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     6781 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     6841 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     6901 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     6961 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     7021 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     7081 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     7141 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     7201 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     7261 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     7321 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     7381 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaacgcgt ctggaacaat
     7441 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     7501 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     7561 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     7621 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     7681 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     7741 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     7801 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     7861 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     7921 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     7981 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct
     8041 gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
     8101 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     8161 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     8221 aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt
     8281 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
     8341 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
     8401 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
     8461 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
     8521 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
     8581 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
     8641 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
     8701 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     8761 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     8821 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     8881 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct
     8941 tgacattgct agcgtttacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     9001 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     9061 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     9121 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     9181 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     9241 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     9301 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     9361 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     9421 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9481 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9541 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9601 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9661 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     9721 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9781 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     9841 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9901 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9961 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    10021 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    10081 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    10141 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    10201 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    10261 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    10321 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    10381 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    10441 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    10501 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    10561 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    10621 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    10681 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    10741 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    10801 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    10861 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    10921 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    10981 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    11041 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    11101 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
    11161 ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    11221 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    11281 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
    11341 tcccacccca ta
//
